Radachlorin-Containing Microparticles for Photodynamic Therapy

Purpose: Reducing the undesirable systemic effect of photodynamic therapy (PDT) can be achieved by incorporating a photosensitizer in microparticles (MPs). This study is devoted to the preparation of biocompatible biodegradable MPs with the inclusion of the natural photosensitizer Radachlorin (RС) a...

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Autores principales:	Sergey Petrovich Krechetov, Anastasia Maksimovna Miroshkina, Maria Nikolaevna Yakovtseva, Elizaveta Nikitichna Mochalova, Andrey Vadimovich Babenyshev, Ivan Vladimirovich Maslov, Alexander Alexandrovich Loshkarev, Ivan Ivanovich Krasnyuk
Formato:	article
Lenguaje:	EN
Publicado:	Tabriz University of Medical Sciences 2020
Materias:	drug delivery systems microparticles radachlorin photodynamic therapy photosensitizing agents tumor cell line Therapeutics. Pharmacology RM1-950
Acceso en línea:	https://doaj.org/article/ce45c53d0adc4b42b6d581c27d471646
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spelling	oai:doaj.org-article:ce45c53d0adc4b42b6d581c27d4716462021-12-05T09:40:24ZRadachlorin-Containing Microparticles for Photodynamic Therapy2228-58812251-730810.34172/apb.2021.053https://doaj.org/article/ce45c53d0adc4b42b6d581c27d4716462020-08-01T00:00:00Zhttps://apb.tbzmed.ac.ir/PDF/apb-11-458.pdfhttps://doaj.org/toc/2228-5881https://doaj.org/toc/2251-7308Purpose: Reducing the undesirable systemic effect of photodynamic therapy (PDT) can be achieved by incorporating a photosensitizer in microparticles (MPs). This study is devoted to the preparation of biocompatible biodegradable MPs with the inclusion of the natural photosensitizer Radachlorin (RС) and an assessment of the possibility of their use for PDT. Methods: RC-containing MPs (RС MPs) with poly(lactic-co-glycolic acid) copolymer (PLGA) matrix were prepared by a double emulsion solvent evaporation methods. The size and morphology of RC MPs were surveyed using scanning electron microscopy, confocal laser scanning microscopy, and dynamic light scattering. The content of RC, its release from RC MPs, and singlet oxygen generation were evaluated by the optical spectroscopy. Cellular uptake and cytotoxic photodynamic effect of RC MPs were investigated with in vitro assays. Results: The average diameter of the prepared RC MPs was about 2-3 μm. The RC MPs prepared by the water/oil/oil method had a significantly higher inclusion of RC (1.74 μg/mg) then RC MPs prepared by the water/oil/water method (0.089 μg/mg). Exposure of the prepared RC MPs to PDT light radiation was accompanied by the singlet oxygen generation and a cytotoxic effect for tumor cells. The release of the RC from the RC MPs was prolonged and lasted at least two weeks. Conclusion: PLGA RC MPs were found to cause a photoactivated cytotoxic effect for tumor cells and can be used for local application in PDT of tumors.Sergey Petrovich KrechetovAnastasia Maksimovna MiroshkinaMaria Nikolaevna YakovtsevaElizaveta Nikitichna MochalovaAndrey Vadimovich BabenyshevIvan Vladimirovich MaslovAlexander Alexandrovich LoshkarevIvan Ivanovich KrasnyukTabriz University of Medical Sciences articledrug delivery systemsmicroparticlesradachlorinphotodynamic therapyphotosensitizing agentstumor cell lineTherapeutics. PharmacologyRM1-950ENAdvanced Pharmaceutical Bulletin, Vol 11, Iss 3, Pp 458-468 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	drug delivery systems microparticles radachlorin photodynamic therapy photosensitizing agents tumor cell line Therapeutics. Pharmacology RM1-950
spellingShingle	drug delivery systems microparticles radachlorin photodynamic therapy photosensitizing agents tumor cell line Therapeutics. Pharmacology RM1-950 Sergey Petrovich Krechetov Anastasia Maksimovna Miroshkina Maria Nikolaevna Yakovtseva Elizaveta Nikitichna Mochalova Andrey Vadimovich Babenyshev Ivan Vladimirovich Maslov Alexander Alexandrovich Loshkarev Ivan Ivanovich Krasnyuk Radachlorin-Containing Microparticles for Photodynamic Therapy
description	Purpose: Reducing the undesirable systemic effect of photodynamic therapy (PDT) can be achieved by incorporating a photosensitizer in microparticles (MPs). This study is devoted to the preparation of biocompatible biodegradable MPs with the inclusion of the natural photosensitizer Radachlorin (RС) and an assessment of the possibility of their use for PDT. Methods: RC-containing MPs (RС MPs) with poly(lactic-co-glycolic acid) copolymer (PLGA) matrix were prepared by a double emulsion solvent evaporation methods. The size and morphology of RC MPs were surveyed using scanning electron microscopy, confocal laser scanning microscopy, and dynamic light scattering. The content of RC, its release from RC MPs, and singlet oxygen generation were evaluated by the optical spectroscopy. Cellular uptake and cytotoxic photodynamic effect of RC MPs were investigated with in vitro assays. Results: The average diameter of the prepared RC MPs was about 2-3 μm. The RC MPs prepared by the water/oil/oil method had a significantly higher inclusion of RC (1.74 μg/mg) then RC MPs prepared by the water/oil/water method (0.089 μg/mg). Exposure of the prepared RC MPs to PDT light radiation was accompanied by the singlet oxygen generation and a cytotoxic effect for tumor cells. The release of the RC from the RC MPs was prolonged and lasted at least two weeks. Conclusion: PLGA RC MPs were found to cause a photoactivated cytotoxic effect for tumor cells and can be used for local application in PDT of tumors.
format	article
author	Sergey Petrovich Krechetov Anastasia Maksimovna Miroshkina Maria Nikolaevna Yakovtseva Elizaveta Nikitichna Mochalova Andrey Vadimovich Babenyshev Ivan Vladimirovich Maslov Alexander Alexandrovich Loshkarev Ivan Ivanovich Krasnyuk
author_facet	Sergey Petrovich Krechetov Anastasia Maksimovna Miroshkina Maria Nikolaevna Yakovtseva Elizaveta Nikitichna Mochalova Andrey Vadimovich Babenyshev Ivan Vladimirovich Maslov Alexander Alexandrovich Loshkarev Ivan Ivanovich Krasnyuk
author_sort	Sergey Petrovich Krechetov
title	Radachlorin-Containing Microparticles for Photodynamic Therapy
title_short	Radachlorin-Containing Microparticles for Photodynamic Therapy
title_full	Radachlorin-Containing Microparticles for Photodynamic Therapy
title_fullStr	Radachlorin-Containing Microparticles for Photodynamic Therapy
title_full_unstemmed	Radachlorin-Containing Microparticles for Photodynamic Therapy
title_sort	radachlorin-containing microparticles for photodynamic therapy
publisher	Tabriz University of Medical Sciences
publishDate	2020
url	https://doaj.org/article/ce45c53d0adc4b42b6d581c27d471646
work_keys_str_mv	AT sergeypetrovichkrechetov radachlorincontainingmicroparticlesforphotodynamictherapy AT anastasiamaksimovnamiroshkina radachlorincontainingmicroparticlesforphotodynamictherapy AT marianikolaevnayakovtseva radachlorincontainingmicroparticlesforphotodynamictherapy AT elizavetanikitichnamochalova radachlorincontainingmicroparticlesforphotodynamictherapy AT andreyvadimovichbabenyshev radachlorincontainingmicroparticlesforphotodynamictherapy AT ivanvladimirovichmaslov radachlorincontainingmicroparticlesforphotodynamictherapy AT alexanderalexandrovichloshkarev radachlorincontainingmicroparticlesforphotodynamictherapy AT ivanivanovichkrasnyuk radachlorincontainingmicroparticlesforphotodynamictherapy
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Radachlorin-Containing Microparticles for Photodynamic Therapy

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