m6A Regulator-Mediated Methylation Modification Patterns and Characteristics of Immunity in Blood Leukocytes of COVID-19 Patients

Both RNA N6-methyladenosine (m6A) modification of SARS-CoV-2 and immune characteristics of the human body have been reported to play an important role in COVID-19, but how the m6A methylation modification of leukocytes responds to the virus infection remains unknown. Based on the RNA-seq of 126 samp...

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Autores principales:	Xiangmin Qiu, Xiaoliang Hua, Qianyin Li, Qin Zhou, Juan Chen
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	COVID-19 immune characteristics m6A methylation modification protective model leukocytes Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/ce45e99c651d4028931b946d0d829a69
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spelling	oai:doaj.org-article:ce45e99c651d4028931b946d0d829a692021-12-01T18:15:58Zm6A Regulator-Mediated Methylation Modification Patterns and Characteristics of Immunity in Blood Leukocytes of COVID-19 Patients1664-322410.3389/fimmu.2021.774776https://doaj.org/article/ce45e99c651d4028931b946d0d829a692021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fimmu.2021.774776/fullhttps://doaj.org/toc/1664-3224Both RNA N6-methyladenosine (m6A) modification of SARS-CoV-2 and immune characteristics of the human body have been reported to play an important role in COVID-19, but how the m6A methylation modification of leukocytes responds to the virus infection remains unknown. Based on the RNA-seq of 126 samples from the GEO database, we disclosed that there is a remarkably higher m6A modification level of blood leukocytes in patients with COVID-19 compared to patients without COVID-19, and this difference was related to CD4+ T cells. Two clusters were identified by unsupervised clustering, m6A cluster A characterized by T cell activation had a higher prognosis than m6A cluster B. Elevated metabolism level, blockage of the immune checkpoint, and lower level of m6A score were observed in m6A cluster B. A protective model was constructed based on nine selected genes and it exhibited an excellent predictive value in COVID-19. Further analysis revealed that the protective score was positively correlated to HFD45 and ventilator-free days, while negatively correlated to SOFA score, APACHE-II score, and crp. Our works systematically depicted a complicated correlation between m6A methylation modification and host lymphocytes in patients infected with SARS-CoV-2 and provided a well-performing model to predict the patients’ outcomes.Xiangmin QiuXiaoliang HuaXiaoliang HuaQianyin LiQin ZhouJuan ChenFrontiers Media S.A.articleCOVID-19immune characteristicsm6A methylation modificationprotective modelleukocytesImmunologic diseases. AllergyRC581-607ENFrontiers in Immunology, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	COVID-19 immune characteristics m6A methylation modification protective model leukocytes Immunologic diseases. Allergy RC581-607
spellingShingle	COVID-19 immune characteristics m6A methylation modification protective model leukocytes Immunologic diseases. Allergy RC581-607 Xiangmin Qiu Xiaoliang Hua Xiaoliang Hua Qianyin Li Qin Zhou Juan Chen m6A Regulator-Mediated Methylation Modification Patterns and Characteristics of Immunity in Blood Leukocytes of COVID-19 Patients
description	Both RNA N6-methyladenosine (m6A) modification of SARS-CoV-2 and immune characteristics of the human body have been reported to play an important role in COVID-19, but how the m6A methylation modification of leukocytes responds to the virus infection remains unknown. Based on the RNA-seq of 126 samples from the GEO database, we disclosed that there is a remarkably higher m6A modification level of blood leukocytes in patients with COVID-19 compared to patients without COVID-19, and this difference was related to CD4+ T cells. Two clusters were identified by unsupervised clustering, m6A cluster A characterized by T cell activation had a higher prognosis than m6A cluster B. Elevated metabolism level, blockage of the immune checkpoint, and lower level of m6A score were observed in m6A cluster B. A protective model was constructed based on nine selected genes and it exhibited an excellent predictive value in COVID-19. Further analysis revealed that the protective score was positively correlated to HFD45 and ventilator-free days, while negatively correlated to SOFA score, APACHE-II score, and crp. Our works systematically depicted a complicated correlation between m6A methylation modification and host lymphocytes in patients infected with SARS-CoV-2 and provided a well-performing model to predict the patients’ outcomes.
format	article
author	Xiangmin Qiu Xiaoliang Hua Xiaoliang Hua Qianyin Li Qin Zhou Juan Chen
author_facet	Xiangmin Qiu Xiaoliang Hua Xiaoliang Hua Qianyin Li Qin Zhou Juan Chen
author_sort	Xiangmin Qiu
title	m6A Regulator-Mediated Methylation Modification Patterns and Characteristics of Immunity in Blood Leukocytes of COVID-19 Patients
title_short	m6A Regulator-Mediated Methylation Modification Patterns and Characteristics of Immunity in Blood Leukocytes of COVID-19 Patients
title_full	m6A Regulator-Mediated Methylation Modification Patterns and Characteristics of Immunity in Blood Leukocytes of COVID-19 Patients
title_fullStr	m6A Regulator-Mediated Methylation Modification Patterns and Characteristics of Immunity in Blood Leukocytes of COVID-19 Patients
title_full_unstemmed	m6A Regulator-Mediated Methylation Modification Patterns and Characteristics of Immunity in Blood Leukocytes of COVID-19 Patients
title_sort	m6a regulator-mediated methylation modification patterns and characteristics of immunity in blood leukocytes of covid-19 patients
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/ce45e99c651d4028931b946d0d829a69
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m6A Regulator-Mediated Methylation Modification Patterns and Characteristics of Immunity in Blood Leukocytes of COVID-19 Patients

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