Do fish perceive anaesthetics as aversive?

This study addresses a fundamental question in fish welfare: are the anaesthetics used for fish aversive? Despite years of routine general use of many agents, within both scientific research and aquaculture, there is a paucity of information regarding their tolerance and associated behavioural respo...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Gareth D Readman, Stewart F Owen, Joanna C Murrell, Toby G Knowles
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/ce4f5e5f74eb4b6db236eb1e5e1894af
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:ce4f5e5f74eb4b6db236eb1e5e1894af
record_format	dspace
spelling	oai:doaj.org-article:ce4f5e5f74eb4b6db236eb1e5e1894af2021-11-18T08:54:02ZDo fish perceive anaesthetics as aversive?1932-620310.1371/journal.pone.0073773https://doaj.org/article/ce4f5e5f74eb4b6db236eb1e5e1894af2013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24086294/?tool=EBIhttps://doaj.org/toc/1932-6203This study addresses a fundamental question in fish welfare: are the anaesthetics used for fish aversive? Despite years of routine general use of many agents, within both scientific research and aquaculture, there is a paucity of information regarding their tolerance and associated behavioural responses by fish. This study examined nine of the most commonly used fish anaesthetic agents, and performed preference tests using adult mixed sex zebrafish (Danio rerio), the most commonly held laboratory fish. Video tracking software quantified swimming behaviour related to aversion for each anaesthetic at 50% of its standard recommended dose compared with clean water in a flow-through chemotaxic choice chamber. Results suggest that several commonly used anaesthetics were aversive, including two of the most commonly recommended and used: MS222 (ethyl 3-aminobenzoate methanesulphate) and benzocaine. For ethical best practice, it is recommended that compounds that are aversive, even at low concentration, should no longer be used routinely for anaesthesia or indeed the first step of humane euthanasia of adult zebrafish. Two agents were found not to induce aversive behavioural responses: etomidate and 2,2,2 tribromoethanol. For the millions of adult zebrafish used in laboratories and breeding worldwide, etomidate appears best suited for future routine humane use.Gareth D ReadmanStewart F OwenJoanna C MurrellToby G KnowlesPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 9, p e73773 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Gareth D Readman Stewart F Owen Joanna C Murrell Toby G Knowles Do fish perceive anaesthetics as aversive?
description	This study addresses a fundamental question in fish welfare: are the anaesthetics used for fish aversive? Despite years of routine general use of many agents, within both scientific research and aquaculture, there is a paucity of information regarding their tolerance and associated behavioural responses by fish. This study examined nine of the most commonly used fish anaesthetic agents, and performed preference tests using adult mixed sex zebrafish (Danio rerio), the most commonly held laboratory fish. Video tracking software quantified swimming behaviour related to aversion for each anaesthetic at 50% of its standard recommended dose compared with clean water in a flow-through chemotaxic choice chamber. Results suggest that several commonly used anaesthetics were aversive, including two of the most commonly recommended and used: MS222 (ethyl 3-aminobenzoate methanesulphate) and benzocaine. For ethical best practice, it is recommended that compounds that are aversive, even at low concentration, should no longer be used routinely for anaesthesia or indeed the first step of humane euthanasia of adult zebrafish. Two agents were found not to induce aversive behavioural responses: etomidate and 2,2,2 tribromoethanol. For the millions of adult zebrafish used in laboratories and breeding worldwide, etomidate appears best suited for future routine humane use.
format	article
author	Gareth D Readman Stewart F Owen Joanna C Murrell Toby G Knowles
author_facet	Gareth D Readman Stewart F Owen Joanna C Murrell Toby G Knowles
author_sort	Gareth D Readman
title	Do fish perceive anaesthetics as aversive?
title_short	Do fish perceive anaesthetics as aversive?
title_full	Do fish perceive anaesthetics as aversive?
title_fullStr	Do fish perceive anaesthetics as aversive?
title_full_unstemmed	Do fish perceive anaesthetics as aversive?
title_sort	do fish perceive anaesthetics as aversive?
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/ce4f5e5f74eb4b6db236eb1e5e1894af
work_keys_str_mv	AT garethdreadman dofishperceiveanaestheticsasaversive AT stewartfowen dofishperceiveanaestheticsasaversive AT joannacmurrell dofishperceiveanaestheticsasaversive AT tobygknowles dofishperceiveanaestheticsasaversive
_version_	1718421229894893568

Do fish perceive anaesthetics as aversive?

Ejemplares similares