Dietary Fiber Ameliorates Lipopolysaccharide-Induced Intestinal Barrier Function Damage in Piglets by Modulation of Intestinal Microbiome

ABSTRACT Weaning of piglets is accompanied by intestinal inflammation, impaired intestinal barrier function, and intestinal microflora disorder. Regulating intestinal microflora structure can directly or indirectly affect intestinal health and host growth and development. However, whether dietary fi...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Xiao Sun, Yalei Cui, Yingying Su, Zimin Gao, Xinying Diao, Ju Li, Xiaoyan Zhu, Defeng Li, Zhentian Li, Chengzhang Wang, Yinghua Shi
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2021
Materias:	dietary fiber lipopolysaccharide piglets inflammatory reaction intestinal barrier function intestinal microflora Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/ce5573c4077b442a88e4effda07010b8
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:ce5573c4077b442a88e4effda07010b8
record_format	dspace
spelling	oai:doaj.org-article:ce5573c4077b442a88e4effda07010b82021-12-02T19:36:40ZDietary Fiber Ameliorates Lipopolysaccharide-Induced Intestinal Barrier Function Damage in Piglets by Modulation of Intestinal Microbiome10.1128/mSystems.01374-202379-5077https://doaj.org/article/ce5573c4077b442a88e4effda07010b82021-04-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mSystems.01374-20https://doaj.org/toc/2379-5077ABSTRACT Weaning of piglets is accompanied by intestinal inflammation, impaired intestinal barrier function, and intestinal microflora disorder. Regulating intestinal microflora structure can directly or indirectly affect intestinal health and host growth and development. However, whether dietary fiber (DF) affects the inflammatory response and barrier function by affecting the intestinal microflora and its metabolites is unclear. In this study, we investigated the role of intestinal microflora in relieving immune stress and maintaining homeostasis using piglets with lipopolysaccharide (LPS)-induced intestinal injury as a model. DF improved intestinal morphology and barrier function, inhibited the expression of inflammatory signal pathways (Toll-like receptor 2 [TLR2], TLR4, and NF-κB) and proinflammatory cytokines (interleukin 1β [IL-1β], IL-6, and tumor necrosis factor alpha [TNF-α]), and upregulated the expression of barrier-related genes (encoding claudin-1, occludin, and ZO-1). The contents of proinflammatory cytokines (IL-1β, IL-6, and TNF-α) and the activity of diamine oxidase in plasma were decreased. Meanwhile, DF had a strong effect on the composition and function of intestinal microflora at different taxonomic levels, the relative abundances of cellulolytic bacteria and anti-inflammatory bacteria were increased, and the concentrations of propionate, butyrate, and total short-chain fatty acids (SCFAs) in intestinal contents were increased. In addition, the correlation analysis also revealed the potential relationship between metabolites and certain intestinal microflora, as well as the relationship between metabolites and intestinal morphology, intestinal gene expression, and plasma cytokine levels. These results indicate that DF improves intestinal barrier function, in part, by altering intestinal microbiota composition and increasing the synthesis of SCFAs, which subsequently alleviate local and systemic inflammation. IMPORTANCE Adding DF to the diet of LPS-challenged piglets alleviated intestinal and systemic inflammation, improved intestinal barrier function, and ultimately alleviated the growth retardation of piglets. In addition, the addition of DF significantly increased the relative abundance of SCFA-producing bacteria and the production of SCFAs. We believe that the improvement of growth performance of piglets with LPS-induced injury can be attributed to the beneficial effects of DF on intestinal microflora and SCFAs, which reduced the inflammatory response in piglets, improving intestinal barrier function and enhancing body health. These research results provide a theoretical basis and guidance for the use of specific fiber sources in the diet to improve intestinal health and growth performance of piglets and thus alleviate weaning stress. Our data also provide insights for studying the role of DF in regulating gastrointestinal function in human infants.Xiao SunYalei CuiYingying SuZimin GaoXinying DiaoJu LiXiaoyan ZhuDefeng LiZhentian LiChengzhang WangYinghua ShiAmerican Society for Microbiologyarticledietary fiberlipopolysaccharidepigletsinflammatory reactionintestinal barrier functionintestinal microfloraMicrobiologyQR1-502ENmSystems, Vol 6, Iss 2 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	dietary fiber lipopolysaccharide piglets inflammatory reaction intestinal barrier function intestinal microflora Microbiology QR1-502
spellingShingle	dietary fiber lipopolysaccharide piglets inflammatory reaction intestinal barrier function intestinal microflora Microbiology QR1-502 Xiao Sun Yalei Cui Yingying Su Zimin Gao Xinying Diao Ju Li Xiaoyan Zhu Defeng Li Zhentian Li Chengzhang Wang Yinghua Shi Dietary Fiber Ameliorates Lipopolysaccharide-Induced Intestinal Barrier Function Damage in Piglets by Modulation of Intestinal Microbiome
description	ABSTRACT Weaning of piglets is accompanied by intestinal inflammation, impaired intestinal barrier function, and intestinal microflora disorder. Regulating intestinal microflora structure can directly or indirectly affect intestinal health and host growth and development. However, whether dietary fiber (DF) affects the inflammatory response and barrier function by affecting the intestinal microflora and its metabolites is unclear. In this study, we investigated the role of intestinal microflora in relieving immune stress and maintaining homeostasis using piglets with lipopolysaccharide (LPS)-induced intestinal injury as a model. DF improved intestinal morphology and barrier function, inhibited the expression of inflammatory signal pathways (Toll-like receptor 2 [TLR2], TLR4, and NF-κB) and proinflammatory cytokines (interleukin 1β [IL-1β], IL-6, and tumor necrosis factor alpha [TNF-α]), and upregulated the expression of barrier-related genes (encoding claudin-1, occludin, and ZO-1). The contents of proinflammatory cytokines (IL-1β, IL-6, and TNF-α) and the activity of diamine oxidase in plasma were decreased. Meanwhile, DF had a strong effect on the composition and function of intestinal microflora at different taxonomic levels, the relative abundances of cellulolytic bacteria and anti-inflammatory bacteria were increased, and the concentrations of propionate, butyrate, and total short-chain fatty acids (SCFAs) in intestinal contents were increased. In addition, the correlation analysis also revealed the potential relationship between metabolites and certain intestinal microflora, as well as the relationship between metabolites and intestinal morphology, intestinal gene expression, and plasma cytokine levels. These results indicate that DF improves intestinal barrier function, in part, by altering intestinal microbiota composition and increasing the synthesis of SCFAs, which subsequently alleviate local and systemic inflammation. IMPORTANCE Adding DF to the diet of LPS-challenged piglets alleviated intestinal and systemic inflammation, improved intestinal barrier function, and ultimately alleviated the growth retardation of piglets. In addition, the addition of DF significantly increased the relative abundance of SCFA-producing bacteria and the production of SCFAs. We believe that the improvement of growth performance of piglets with LPS-induced injury can be attributed to the beneficial effects of DF on intestinal microflora and SCFAs, which reduced the inflammatory response in piglets, improving intestinal barrier function and enhancing body health. These research results provide a theoretical basis and guidance for the use of specific fiber sources in the diet to improve intestinal health and growth performance of piglets and thus alleviate weaning stress. Our data also provide insights for studying the role of DF in regulating gastrointestinal function in human infants.
format	article
author	Xiao Sun Yalei Cui Yingying Su Zimin Gao Xinying Diao Ju Li Xiaoyan Zhu Defeng Li Zhentian Li Chengzhang Wang Yinghua Shi
author_facet	Xiao Sun Yalei Cui Yingying Su Zimin Gao Xinying Diao Ju Li Xiaoyan Zhu Defeng Li Zhentian Li Chengzhang Wang Yinghua Shi
author_sort	Xiao Sun
title	Dietary Fiber Ameliorates Lipopolysaccharide-Induced Intestinal Barrier Function Damage in Piglets by Modulation of Intestinal Microbiome
title_short	Dietary Fiber Ameliorates Lipopolysaccharide-Induced Intestinal Barrier Function Damage in Piglets by Modulation of Intestinal Microbiome
title_full	Dietary Fiber Ameliorates Lipopolysaccharide-Induced Intestinal Barrier Function Damage in Piglets by Modulation of Intestinal Microbiome
title_fullStr	Dietary Fiber Ameliorates Lipopolysaccharide-Induced Intestinal Barrier Function Damage in Piglets by Modulation of Intestinal Microbiome
title_full_unstemmed	Dietary Fiber Ameliorates Lipopolysaccharide-Induced Intestinal Barrier Function Damage in Piglets by Modulation of Intestinal Microbiome
title_sort	dietary fiber ameliorates lipopolysaccharide-induced intestinal barrier function damage in piglets by modulation of intestinal microbiome
publisher	American Society for Microbiology
publishDate	2021
url	https://doaj.org/article/ce5573c4077b442a88e4effda07010b8
work_keys_str_mv	AT xiaosun dietaryfiberameliorateslipopolysaccharideinducedintestinalbarrierfunctiondamageinpigletsbymodulationofintestinalmicrobiome AT yaleicui dietaryfiberameliorateslipopolysaccharideinducedintestinalbarrierfunctiondamageinpigletsbymodulationofintestinalmicrobiome AT yingyingsu dietaryfiberameliorateslipopolysaccharideinducedintestinalbarrierfunctiondamageinpigletsbymodulationofintestinalmicrobiome AT zimingao dietaryfiberameliorateslipopolysaccharideinducedintestinalbarrierfunctiondamageinpigletsbymodulationofintestinalmicrobiome AT xinyingdiao dietaryfiberameliorateslipopolysaccharideinducedintestinalbarrierfunctiondamageinpigletsbymodulationofintestinalmicrobiome AT juli dietaryfiberameliorateslipopolysaccharideinducedintestinalbarrierfunctiondamageinpigletsbymodulationofintestinalmicrobiome AT xiaoyanzhu dietaryfiberameliorateslipopolysaccharideinducedintestinalbarrierfunctiondamageinpigletsbymodulationofintestinalmicrobiome AT defengli dietaryfiberameliorateslipopolysaccharideinducedintestinalbarrierfunctiondamageinpigletsbymodulationofintestinalmicrobiome AT zhentianli dietaryfiberameliorateslipopolysaccharideinducedintestinalbarrierfunctiondamageinpigletsbymodulationofintestinalmicrobiome AT chengzhangwang dietaryfiberameliorateslipopolysaccharideinducedintestinalbarrierfunctiondamageinpigletsbymodulationofintestinalmicrobiome AT yinghuashi dietaryfiberameliorateslipopolysaccharideinducedintestinalbarrierfunctiondamageinpigletsbymodulationofintestinalmicrobiome
_version_	1718376350951145472

Dietary Fiber Ameliorates Lipopolysaccharide-Induced Intestinal Barrier Function Damage in Piglets by Modulation of Intestinal Microbiome

Ejemplares similares