miR-513b-5p inhibits the proliferation and promotes apoptosis of retinoblastoma cells by targeting TRIB1

MicroRNAs are involved in the pathogenesis of various human malignant tumors. This study aims to explore the role of miR-513b-5p in the malignant proliferation of retinoblastoma (RB) cells and its potential molecular mechanisms. The function-gain and function-loss experiments were performed in Weri-...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Zhang Li-Juan, Wang Fang, Qi Pei-Yan, Zhou Wei-Yan, Wang Bing
Formato:	article
Lenguaje:	EN
Publicado:	De Gruyter 2021
Materias:	mir-513b-5p trib1 weri-rb1 cells proliferation apoptosis retinoblastoma Medicine R
Acceso en línea:	https://doaj.org/article/ce55f42121fc4843afc2e1d6db67141a
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:ce55f42121fc4843afc2e1d6db67141a
record_format	dspace
spelling	oai:doaj.org-article:ce55f42121fc4843afc2e1d6db67141a2021-12-05T14:10:55ZmiR-513b-5p inhibits the proliferation and promotes apoptosis of retinoblastoma cells by targeting TRIB12391-546310.1515/med-2021-0343https://doaj.org/article/ce55f42121fc4843afc2e1d6db67141a2021-09-01T00:00:00Zhttps://doi.org/10.1515/med-2021-0343https://doaj.org/toc/2391-5463MicroRNAs are involved in the pathogenesis of various human malignant tumors. This study aims to explore the role of miR-513b-5p in the malignant proliferation of retinoblastoma (RB) cells and its potential molecular mechanisms. The function-gain and function-loss experiments were performed in Weri-RB1 cells using miR-513b-5 mimics and inhibitors. miR-513b-5p mimics inhibited the proliferation and clone formation and promoted apoptosis of Weri-RB1 cells. In contrast, the miR-513b-5p inhibitor promoted the proliferation and clone formation of Weri-RB1 cells and inhibited cell apoptosis. miR-513b-5p can directly bind to the 3′UTR region of TRIB1 mRNA, and inhibit its protein expression. Overexpression of TRIB1 promoted the proliferation and cloning of Weri-RB1 cells but inhibited their apoptosis. The knockdown of TRIB1 inhibited the proliferation and clone formation of Weri-RB1 cells and promoted cell apoptosis. In addition, miR-513b-5p mimics neutralized the effects of TRIB1 overexpression on the proliferation and apoptosis of Weri-RB1 cells. Finally, miR-513b-5p can inhibit the phosphorylation level of AKT, mTOR, and p70, while TRIB1 played the opposite role. miR-513b-5p inhibits the malignant proliferation of Weri-RB1 cells by repressing the expression of TRIB1. miR-513b-5p and TRIB1 may be the biomarkers and/or key targets for clinical diagnosis and treatment of RB.Zhang Li-JuanWang FangQi Pei-YanZhou Wei-YanWang BingDe Gruyterarticlemir-513b-5ptrib1weri-rb1 cellsproliferationapoptosisretinoblastomaMedicineRENOpen Medicine, Vol 16, Iss 1, Pp 1364-1371 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	mir-513b-5p trib1 weri-rb1 cells proliferation apoptosis retinoblastoma Medicine R
spellingShingle	mir-513b-5p trib1 weri-rb1 cells proliferation apoptosis retinoblastoma Medicine R Zhang Li-Juan Wang Fang Qi Pei-Yan Zhou Wei-Yan Wang Bing miR-513b-5p inhibits the proliferation and promotes apoptosis of retinoblastoma cells by targeting TRIB1
description	MicroRNAs are involved in the pathogenesis of various human malignant tumors. This study aims to explore the role of miR-513b-5p in the malignant proliferation of retinoblastoma (RB) cells and its potential molecular mechanisms. The function-gain and function-loss experiments were performed in Weri-RB1 cells using miR-513b-5 mimics and inhibitors. miR-513b-5p mimics inhibited the proliferation and clone formation and promoted apoptosis of Weri-RB1 cells. In contrast, the miR-513b-5p inhibitor promoted the proliferation and clone formation of Weri-RB1 cells and inhibited cell apoptosis. miR-513b-5p can directly bind to the 3′UTR region of TRIB1 mRNA, and inhibit its protein expression. Overexpression of TRIB1 promoted the proliferation and cloning of Weri-RB1 cells but inhibited their apoptosis. The knockdown of TRIB1 inhibited the proliferation and clone formation of Weri-RB1 cells and promoted cell apoptosis. In addition, miR-513b-5p mimics neutralized the effects of TRIB1 overexpression on the proliferation and apoptosis of Weri-RB1 cells. Finally, miR-513b-5p can inhibit the phosphorylation level of AKT, mTOR, and p70, while TRIB1 played the opposite role. miR-513b-5p inhibits the malignant proliferation of Weri-RB1 cells by repressing the expression of TRIB1. miR-513b-5p and TRIB1 may be the biomarkers and/or key targets for clinical diagnosis and treatment of RB.
format	article
author	Zhang Li-Juan Wang Fang Qi Pei-Yan Zhou Wei-Yan Wang Bing
author_facet	Zhang Li-Juan Wang Fang Qi Pei-Yan Zhou Wei-Yan Wang Bing
author_sort	Zhang Li-Juan
title	miR-513b-5p inhibits the proliferation and promotes apoptosis of retinoblastoma cells by targeting TRIB1
title_short	miR-513b-5p inhibits the proliferation and promotes apoptosis of retinoblastoma cells by targeting TRIB1
title_full	miR-513b-5p inhibits the proliferation and promotes apoptosis of retinoblastoma cells by targeting TRIB1
title_fullStr	miR-513b-5p inhibits the proliferation and promotes apoptosis of retinoblastoma cells by targeting TRIB1
title_full_unstemmed	miR-513b-5p inhibits the proliferation and promotes apoptosis of retinoblastoma cells by targeting TRIB1
title_sort	mir-513b-5p inhibits the proliferation and promotes apoptosis of retinoblastoma cells by targeting trib1
publisher	De Gruyter
publishDate	2021
url	https://doaj.org/article/ce55f42121fc4843afc2e1d6db67141a
work_keys_str_mv	AT zhanglijuan mir513b5pinhibitstheproliferationandpromotesapoptosisofretinoblastomacellsbytargetingtrib1 AT wangfang mir513b5pinhibitstheproliferationandpromotesapoptosisofretinoblastomacellsbytargetingtrib1 AT qipeiyan mir513b5pinhibitstheproliferationandpromotesapoptosisofretinoblastomacellsbytargetingtrib1 AT zhouweiyan mir513b5pinhibitstheproliferationandpromotesapoptosisofretinoblastomacellsbytargetingtrib1 AT wangbing mir513b5pinhibitstheproliferationandpromotesapoptosisofretinoblastomacellsbytargetingtrib1
_version_	1718371628579028992

miR-513b-5p inhibits the proliferation and promotes apoptosis of retinoblastoma cells by targeting TRIB1

Ejemplares similares