Effect of membrane fusion protein AdeT1 on the antimicrobial resistance of Escherichia coli

Abstract Acinetobacter baumannii is a prevalent pathogen that can rapidly acquire resistance to antibiotics. Indeed, multidrug-resistant A. baumannii is a major cause of hospital-acquired infections and has been recognised by the World Health Organization as one of the most threatening bacteria to o...

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Autores principales:	Victoria L. Barlow, Shu-Jung Lai, Chia-Yu Chen, Cheng-Han Tsai, Shih-Hsiung Wu, Yu-Hsuan Tsai
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/ce57268804764112802f4b22925631a1
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spelling	oai:doaj.org-article:ce57268804764112802f4b22925631a12021-12-02T15:09:48ZEffect of membrane fusion protein AdeT1 on the antimicrobial resistance of Escherichia coli10.1038/s41598-020-77339-w2045-2322https://doaj.org/article/ce57268804764112802f4b22925631a12020-11-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-77339-whttps://doaj.org/toc/2045-2322Abstract Acinetobacter baumannii is a prevalent pathogen that can rapidly acquire resistance to antibiotics. Indeed, multidrug-resistant A. baumannii is a major cause of hospital-acquired infections and has been recognised by the World Health Organization as one of the most threatening bacteria to our society. Resistance-nodulation-division (RND) type multidrug efflux pumps have been demonstrated to convey antibiotic resistance to a wide range of pathogens and are the primary resistance mechanism employed by A. baumannii. A component of an RND pump in A. baumannii, AdeT1, was previously demonstrated to enhance the antimicrobial resistance of Escherichia coli. Here, we report the results of experiments which demonstrate that wild-type AdeT1 does not confer antimicrobial resistance in E. coli, highlighting the importance of verifying protein production when determining minimum inhibitory concentrations (MICs) especially by broth dilution. Nevertheless, using an agar-based MIC assay, we found that propionylation of Lys280 on AdeT1 renders E. coli cells more resistant to erythromycin.Victoria L. BarlowShu-Jung LaiChia-Yu ChenCheng-Han TsaiShih-Hsiung WuYu-Hsuan TsaiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-13 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Victoria L. Barlow Shu-Jung Lai Chia-Yu Chen Cheng-Han Tsai Shih-Hsiung Wu Yu-Hsuan Tsai Effect of membrane fusion protein AdeT1 on the antimicrobial resistance of Escherichia coli
description	Abstract Acinetobacter baumannii is a prevalent pathogen that can rapidly acquire resistance to antibiotics. Indeed, multidrug-resistant A. baumannii is a major cause of hospital-acquired infections and has been recognised by the World Health Organization as one of the most threatening bacteria to our society. Resistance-nodulation-division (RND) type multidrug efflux pumps have been demonstrated to convey antibiotic resistance to a wide range of pathogens and are the primary resistance mechanism employed by A. baumannii. A component of an RND pump in A. baumannii, AdeT1, was previously demonstrated to enhance the antimicrobial resistance of Escherichia coli. Here, we report the results of experiments which demonstrate that wild-type AdeT1 does not confer antimicrobial resistance in E. coli, highlighting the importance of verifying protein production when determining minimum inhibitory concentrations (MICs) especially by broth dilution. Nevertheless, using an agar-based MIC assay, we found that propionylation of Lys280 on AdeT1 renders E. coli cells more resistant to erythromycin.
format	article
author	Victoria L. Barlow Shu-Jung Lai Chia-Yu Chen Cheng-Han Tsai Shih-Hsiung Wu Yu-Hsuan Tsai
author_facet	Victoria L. Barlow Shu-Jung Lai Chia-Yu Chen Cheng-Han Tsai Shih-Hsiung Wu Yu-Hsuan Tsai
author_sort	Victoria L. Barlow
title	Effect of membrane fusion protein AdeT1 on the antimicrobial resistance of Escherichia coli
title_short	Effect of membrane fusion protein AdeT1 on the antimicrobial resistance of Escherichia coli
title_full	Effect of membrane fusion protein AdeT1 on the antimicrobial resistance of Escherichia coli
title_fullStr	Effect of membrane fusion protein AdeT1 on the antimicrobial resistance of Escherichia coli
title_full_unstemmed	Effect of membrane fusion protein AdeT1 on the antimicrobial resistance of Escherichia coli
title_sort	effect of membrane fusion protein adet1 on the antimicrobial resistance of escherichia coli
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/ce57268804764112802f4b22925631a1
work_keys_str_mv	AT victorialbarlow effectofmembranefusionproteinadet1ontheantimicrobialresistanceofescherichiacoli AT shujunglai effectofmembranefusionproteinadet1ontheantimicrobialresistanceofescherichiacoli AT chiayuchen effectofmembranefusionproteinadet1ontheantimicrobialresistanceofescherichiacoli AT chenghantsai effectofmembranefusionproteinadet1ontheantimicrobialresistanceofescherichiacoli AT shihhsiungwu effectofmembranefusionproteinadet1ontheantimicrobialresistanceofescherichiacoli AT yuhsuantsai effectofmembranefusionproteinadet1ontheantimicrobialresistanceofescherichiacoli
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Effect of membrane fusion protein AdeT1 on the antimicrobial resistance of Escherichia coli

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