Transcriptome sequencing of Salvia miltiorrhiza after infection by its endophytic fungi and identification of genes related to tanshinone biosynthesis

Context: Salvia miltiorrhiza Bunge (Labiatae) is a traditional Chinese herb. Endophytic fungi, which are biotic elicitors, can induce accumulation of secondary metabolites in their host plants. Objective: To analyze the interaction mechanism between S. miltiorrhiza and endophytic fungi. Materials an...

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Autores principales:	Yan Jiang, Lei Wang, Shaorong Lu, Yizhe Xue, Xiying Wei, Juan Lu, Yanyan Zhang
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2019
Materias:	interaction metabolic production plant stress mva pathway mep pathway degs Therapeutics. Pharmacology RM1-950
Acceso en línea:	https://doaj.org/article/ce5a38b1dd3143139dc8d6511c3e440e
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spelling	oai:doaj.org-article:ce5a38b1dd3143139dc8d6511c3e440e2021-11-17T14:21:56ZTranscriptome sequencing of Salvia miltiorrhiza after infection by its endophytic fungi and identification of genes related to tanshinone biosynthesis1388-02091744-511610.1080/13880209.2019.1680706https://doaj.org/article/ce5a38b1dd3143139dc8d6511c3e440e2019-01-01T00:00:00Zhttp://dx.doi.org/10.1080/13880209.2019.1680706https://doaj.org/toc/1388-0209https://doaj.org/toc/1744-5116Context: Salvia miltiorrhiza Bunge (Labiatae) is a traditional Chinese herb. Endophytic fungi, which are biotic elicitors, can induce accumulation of secondary metabolites in their host plants. Objective: To analyze the interaction mechanism between S. miltiorrhiza and endophytic fungi. Materials and methods: Endophytic fungi U104 producing tanshinone IIA were isolated from the healthy disease-free tissue of root of S. miltiorrhiza by conventional methods. The endophytic fungus U104 of S. miltiorrhiza was co-cultured with the sterile seedlings of S. miltiorrhiza for 20 d (temp:day/night = 26 °C/18 °C, photoperiod:12/12 h, illuminance:2000 Lx). Transcriptome sequencing of S. miltiorrhiza seedlings after 20 d of co-cultivation was performed using the Illumina platform. Results: A total of 3713 differentially expressed genes (DEGs) were obtained. These different expression genes, such as STPII, LTP2, MYB transcription factors, CNGC, CDPK, Rboh, CaM, MAP2K1/MEK1, WRKY33, SGT1/SGT and Hsp90/htpG, showed that host S. miltiorrhiza had biological defence response in the initial stage of interaction. Under the induction of endophytic fungi, 14 key enzyme genes were up-regulated in the tanshinone biosynthesis pathway: DXS, DXS2, DXR, HMGR3, AACT, MK, PMK, GGPPS2, GPPS, KSL, IDI, IPII, FDPS and CPS. Discussion and conclusions: A total of 14 key genes were obtained from the tanshinone component synthesis and metabolic pathways, providing a reasonable explanation for the accumulation of tanshinone components, an accumulation induced by endophytic fungi, in the host plants. The large amounts of data generated in this study provide a strong and powerful platform for future functional and molecular studies of interactions between host plants and their endophytic fungi.Yan JiangLei WangShaorong LuYizhe XueXiying WeiJuan LuYanyan ZhangTaylor & Francis Grouparticleinteractionmetabolic productionplant stressmva pathwaymep pathwaydegsTherapeutics. PharmacologyRM1-950ENPharmaceutical Biology, Vol 57, Iss 1, Pp 760-769 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	interaction metabolic production plant stress mva pathway mep pathway degs Therapeutics. Pharmacology RM1-950
spellingShingle	interaction metabolic production plant stress mva pathway mep pathway degs Therapeutics. Pharmacology RM1-950 Yan Jiang Lei Wang Shaorong Lu Yizhe Xue Xiying Wei Juan Lu Yanyan Zhang Transcriptome sequencing of Salvia miltiorrhiza after infection by its endophytic fungi and identification of genes related to tanshinone biosynthesis
description	Context: Salvia miltiorrhiza Bunge (Labiatae) is a traditional Chinese herb. Endophytic fungi, which are biotic elicitors, can induce accumulation of secondary metabolites in their host plants. Objective: To analyze the interaction mechanism between S. miltiorrhiza and endophytic fungi. Materials and methods: Endophytic fungi U104 producing tanshinone IIA were isolated from the healthy disease-free tissue of root of S. miltiorrhiza by conventional methods. The endophytic fungus U104 of S. miltiorrhiza was co-cultured with the sterile seedlings of S. miltiorrhiza for 20 d (temp:day/night = 26 °C/18 °C, photoperiod:12/12 h, illuminance:2000 Lx). Transcriptome sequencing of S. miltiorrhiza seedlings after 20 d of co-cultivation was performed using the Illumina platform. Results: A total of 3713 differentially expressed genes (DEGs) were obtained. These different expression genes, such as STPII, LTP2, MYB transcription factors, CNGC, CDPK, Rboh, CaM, MAP2K1/MEK1, WRKY33, SGT1/SGT and Hsp90/htpG, showed that host S. miltiorrhiza had biological defence response in the initial stage of interaction. Under the induction of endophytic fungi, 14 key enzyme genes were up-regulated in the tanshinone biosynthesis pathway: DXS, DXS2, DXR, HMGR3, AACT, MK, PMK, GGPPS2, GPPS, KSL, IDI, IPII, FDPS and CPS. Discussion and conclusions: A total of 14 key genes were obtained from the tanshinone component synthesis and metabolic pathways, providing a reasonable explanation for the accumulation of tanshinone components, an accumulation induced by endophytic fungi, in the host plants. The large amounts of data generated in this study provide a strong and powerful platform for future functional and molecular studies of interactions between host plants and their endophytic fungi.
format	article
author	Yan Jiang Lei Wang Shaorong Lu Yizhe Xue Xiying Wei Juan Lu Yanyan Zhang
author_facet	Yan Jiang Lei Wang Shaorong Lu Yizhe Xue Xiying Wei Juan Lu Yanyan Zhang
author_sort	Yan Jiang
title	Transcriptome sequencing of Salvia miltiorrhiza after infection by its endophytic fungi and identification of genes related to tanshinone biosynthesis
title_short	Transcriptome sequencing of Salvia miltiorrhiza after infection by its endophytic fungi and identification of genes related to tanshinone biosynthesis
title_full	Transcriptome sequencing of Salvia miltiorrhiza after infection by its endophytic fungi and identification of genes related to tanshinone biosynthesis
title_fullStr	Transcriptome sequencing of Salvia miltiorrhiza after infection by its endophytic fungi and identification of genes related to tanshinone biosynthesis
title_full_unstemmed	Transcriptome sequencing of Salvia miltiorrhiza after infection by its endophytic fungi and identification of genes related to tanshinone biosynthesis
title_sort	transcriptome sequencing of salvia miltiorrhiza after infection by its endophytic fungi and identification of genes related to tanshinone biosynthesis
publisher	Taylor & Francis Group
publishDate	2019
url	https://doaj.org/article/ce5a38b1dd3143139dc8d6511c3e440e
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Transcriptome sequencing of Salvia miltiorrhiza after infection by its endophytic fungi and identification of genes related to tanshinone biosynthesis

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