Green synthesis of silver nanoparticles using cranberry powder aqueous extract: characterization and antimicrobial properties

Asmaa A Ashour,1 Dina Raafat,2 Hanan M El-Gowelli,3 Amal H El-Kamel1 1Department of Pharmaceutics, 2Department of Pharmaceutical Microbiology, 3Department of Pharmacology and Toxicology, Faculty of Pharmacy, Alexandria University, Alexandria, Egypt Background: The growing threat of microbial resista...

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Autores principales:	Ashour AA, Raafat D, El-Gowelli HM, El-Kamel AH
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2015
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/ce5bd74740d3435aa89fc801b8568f83
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spelling	oai:doaj.org-article:ce5bd74740d3435aa89fc801b8568f832021-12-02T03:10:57ZGreen synthesis of silver nanoparticles using cranberry powder aqueous extract: characterization and antimicrobial properties1178-2013https://doaj.org/article/ce5bd74740d3435aa89fc801b8568f832015-12-01T00:00:00Zhttps://www.dovepress.com/green-synthesis-of-silver-nanoparticles-using-cranberry-powder-aqueous-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Asmaa A Ashour,1 Dina Raafat,2 Hanan M El-Gowelli,3 Amal H El-Kamel1 1Department of Pharmaceutics, 2Department of Pharmaceutical Microbiology, 3Department of Pharmacology and Toxicology, Faculty of Pharmacy, Alexandria University, Alexandria, Egypt Background: The growing threat of microbial resistance against traditional antibiotics has prompted the development of several antimicrobial nanoparticles (NPs), including silver NPs (AgNPs). In this article, a simple and eco-friendly method for the synthesis of AgNPs using the cranberry powder aqueous extract is reported.Materials and methods: Cranberry powder aqueous extracts (0.2%, 0.5%, and 0.8% w/v) were allowed to interact for 24 hours with a silver nitrate solution (10 mM) at 30°C at a ratio of 1:10. The formation of AgNPs was confirmed by ultraviolet-visible spectroscopy and their concentrations were determined using atomic absorption spectroscopy. The prepared NPs were evaluated by transmission electron microscopy, measurement of ζ-potential, and Fourier-transform infrared spectroscopy. The in vitro antimicrobial properties of AgNPs were then investigated against several microbial strains. Finally, in vivo appraisal of both wound-healing and antimicrobial properties of either plain AgNPs (prepared using 0.2% extract) or AgNP-Pluronic F-127 gel was conducted in a rat model after induction of a Staphylococcus aureus ATCC 6538P wound infection.Results: The formation of AgNPs was confirmed by ultraviolet-visible spectroscopy, where a surface-plasmon resonance absorption peak was observed between 432 and 438 nm. Both size and concentration of the formed AgNPs increased with increasing concentration of the extracts. The developed NPs were stable, almost spherical, and polydisperse, with a size range of 1.4–8.6 nm. The negative ζ-potential values, as well as Fourier-transform infrared spectroscopy analysis, indicated the presence of a capping agent adsorbed onto the surface of the particles. In vitro antimicrobial evaluation revealed a size-dependent activity of the AgNPs against the tested organisms. Finally, AgNPs prepared using 0.2% extract exhibited a substantial in vivo healing potential for full-thickness excision wounds in rats.Conclusion: AgNPs were successfully synthesized from a silver nitrate solution through a simple green route, using cranberry powder aqueous extract as a reducing as well as capping agent. Keywords: cranberry, silver nanoparticles, antimicrobial properties, wound healingAshour AARaafat DEl-Gowelli HMEl-Kamel AHDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2015, Iss default, Pp 7207-7221 (2015)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Ashour AA Raafat D El-Gowelli HM El-Kamel AH Green synthesis of silver nanoparticles using cranberry powder aqueous extract: characterization and antimicrobial properties
description	Asmaa A Ashour,1 Dina Raafat,2 Hanan M El-Gowelli,3 Amal H El-Kamel1 1Department of Pharmaceutics, 2Department of Pharmaceutical Microbiology, 3Department of Pharmacology and Toxicology, Faculty of Pharmacy, Alexandria University, Alexandria, Egypt Background: The growing threat of microbial resistance against traditional antibiotics has prompted the development of several antimicrobial nanoparticles (NPs), including silver NPs (AgNPs). In this article, a simple and eco-friendly method for the synthesis of AgNPs using the cranberry powder aqueous extract is reported.Materials and methods: Cranberry powder aqueous extracts (0.2%, 0.5%, and 0.8% w/v) were allowed to interact for 24 hours with a silver nitrate solution (10 mM) at 30°C at a ratio of 1:10. The formation of AgNPs was confirmed by ultraviolet-visible spectroscopy and their concentrations were determined using atomic absorption spectroscopy. The prepared NPs were evaluated by transmission electron microscopy, measurement of ζ-potential, and Fourier-transform infrared spectroscopy. The in vitro antimicrobial properties of AgNPs were then investigated against several microbial strains. Finally, in vivo appraisal of both wound-healing and antimicrobial properties of either plain AgNPs (prepared using 0.2% extract) or AgNP-Pluronic F-127 gel was conducted in a rat model after induction of a Staphylococcus aureus ATCC 6538P wound infection.Results: The formation of AgNPs was confirmed by ultraviolet-visible spectroscopy, where a surface-plasmon resonance absorption peak was observed between 432 and 438 nm. Both size and concentration of the formed AgNPs increased with increasing concentration of the extracts. The developed NPs were stable, almost spherical, and polydisperse, with a size range of 1.4–8.6 nm. The negative ζ-potential values, as well as Fourier-transform infrared spectroscopy analysis, indicated the presence of a capping agent adsorbed onto the surface of the particles. In vitro antimicrobial evaluation revealed a size-dependent activity of the AgNPs against the tested organisms. Finally, AgNPs prepared using 0.2% extract exhibited a substantial in vivo healing potential for full-thickness excision wounds in rats.Conclusion: AgNPs were successfully synthesized from a silver nitrate solution through a simple green route, using cranberry powder aqueous extract as a reducing as well as capping agent. Keywords: cranberry, silver nanoparticles, antimicrobial properties, wound healing
format	article
author	Ashour AA Raafat D El-Gowelli HM El-Kamel AH
author_facet	Ashour AA Raafat D El-Gowelli HM El-Kamel AH
author_sort	Ashour AA
title	Green synthesis of silver nanoparticles using cranberry powder aqueous extract: characterization and antimicrobial properties
title_short	Green synthesis of silver nanoparticles using cranberry powder aqueous extract: characterization and antimicrobial properties
title_full	Green synthesis of silver nanoparticles using cranberry powder aqueous extract: characterization and antimicrobial properties
title_fullStr	Green synthesis of silver nanoparticles using cranberry powder aqueous extract: characterization and antimicrobial properties
title_full_unstemmed	Green synthesis of silver nanoparticles using cranberry powder aqueous extract: characterization and antimicrobial properties
title_sort	green synthesis of silver nanoparticles using cranberry powder aqueous extract: characterization and antimicrobial properties
publisher	Dove Medical Press
publishDate	2015
url	https://doaj.org/article/ce5bd74740d3435aa89fc801b8568f83
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Green synthesis of silver nanoparticles using cranberry powder aqueous extract: characterization and antimicrobial properties

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