Application of culture, PCR, and PacBio sequencing for determination of microbial composition of milk from subclinical mastitis dairy cows of smallholder farms

Mastitis is a cow disease usually signalized by irritation, swelling, and soreness of the udder. It is characterized by physical, chemical, and biological changes in the udder and milk. The aim of this study was to detect and characterize pathogens causing subclinical mastitis (SCM) from the milk of...

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Autores principales:	Khasapane Ntelekwane G., Nkhebenyane Jane S., Kwenda Stanford, Khumalo Zamantungwa T. H., Mtshali Phillip S., Taioe Moeti O., Thekisoe Oriel M. M.
Formato:	article
Lenguaje:	EN
Publicado:	De Gruyter 2021
Materias:	microbiota mastitis 16s ribosomal rna dna sequencing microbial milk composition Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/ce62630c607247ddad7863fe44cd73e2
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spelling	oai:doaj.org-article:ce62630c607247ddad7863fe44cd73e22021-12-05T14:10:41ZApplication of culture, PCR, and PacBio sequencing for determination of microbial composition of milk from subclinical mastitis dairy cows of smallholder farms2391-541210.1515/biol-2021-0080https://doaj.org/article/ce62630c607247ddad7863fe44cd73e22021-08-01T00:00:00Zhttps://doi.org/10.1515/biol-2021-0080https://doaj.org/toc/2391-5412Mastitis is a cow disease usually signalized by irritation, swelling, and soreness of the udder. It is characterized by physical, chemical, and biological changes in the udder and milk. The aim of this study was to detect and characterize pathogens causing subclinical mastitis (SCM) from the milk of dairy cows of small-scale farmers through culture and molecular techniques. Milk was collected from 32 cows belonging to 8 small-scale farmers around Harrismith District, South Africa. The results showed that screening of SCM by California mastitis test and somatic cell counts (SCC) was 21.87 and 25%, respectively. Culture methods revealed the presence of Staphylococcus aureus at 93% followed by Streptococci spp. and Escherichia coli at 36.4 and 13.3%, respectively. The PCR could only detect E. coli, while single-molecule real-time sequencing showed a total of 2 phyla, 5 families, 7 genera, and 131 species. Clostridiaceae was the most abundant family, while Romboutsia was the most abundant genus followed by Turicibacter spp. The present study has documented the occurrence of SCM causing pathogens in milk collected from cows of small-scale farmers in Harrismith, indicating that SCM may be present at higher levels than expected.Khasapane Ntelekwane G.Nkhebenyane Jane S.Kwenda StanfordKhumalo Zamantungwa T. H.Mtshali Phillip S.Taioe Moeti O.Thekisoe Oriel M. M.De Gruyterarticlemicrobiotamastitis16s ribosomal rnadna sequencingmicrobial milk compositionBiology (General)QH301-705.5ENOpen Life Sciences, Vol 16, Iss 1, Pp 800-808 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	microbiota mastitis 16s ribosomal rna dna sequencing microbial milk composition Biology (General) QH301-705.5
spellingShingle	microbiota mastitis 16s ribosomal rna dna sequencing microbial milk composition Biology (General) QH301-705.5 Khasapane Ntelekwane G. Nkhebenyane Jane S. Kwenda Stanford Khumalo Zamantungwa T. H. Mtshali Phillip S. Taioe Moeti O. Thekisoe Oriel M. M. Application of culture, PCR, and PacBio sequencing for determination of microbial composition of milk from subclinical mastitis dairy cows of smallholder farms
description	Mastitis is a cow disease usually signalized by irritation, swelling, and soreness of the udder. It is characterized by physical, chemical, and biological changes in the udder and milk. The aim of this study was to detect and characterize pathogens causing subclinical mastitis (SCM) from the milk of dairy cows of small-scale farmers through culture and molecular techniques. Milk was collected from 32 cows belonging to 8 small-scale farmers around Harrismith District, South Africa. The results showed that screening of SCM by California mastitis test and somatic cell counts (SCC) was 21.87 and 25%, respectively. Culture methods revealed the presence of Staphylococcus aureus at 93% followed by Streptococci spp. and Escherichia coli at 36.4 and 13.3%, respectively. The PCR could only detect E. coli, while single-molecule real-time sequencing showed a total of 2 phyla, 5 families, 7 genera, and 131 species. Clostridiaceae was the most abundant family, while Romboutsia was the most abundant genus followed by Turicibacter spp. The present study has documented the occurrence of SCM causing pathogens in milk collected from cows of small-scale farmers in Harrismith, indicating that SCM may be present at higher levels than expected.
format	article
author	Khasapane Ntelekwane G. Nkhebenyane Jane S. Kwenda Stanford Khumalo Zamantungwa T. H. Mtshali Phillip S. Taioe Moeti O. Thekisoe Oriel M. M.
author_facet	Khasapane Ntelekwane G. Nkhebenyane Jane S. Kwenda Stanford Khumalo Zamantungwa T. H. Mtshali Phillip S. Taioe Moeti O. Thekisoe Oriel M. M.
author_sort	Khasapane Ntelekwane G.
title	Application of culture, PCR, and PacBio sequencing for determination of microbial composition of milk from subclinical mastitis dairy cows of smallholder farms
title_short	Application of culture, PCR, and PacBio sequencing for determination of microbial composition of milk from subclinical mastitis dairy cows of smallholder farms
title_full	Application of culture, PCR, and PacBio sequencing for determination of microbial composition of milk from subclinical mastitis dairy cows of smallholder farms
title_fullStr	Application of culture, PCR, and PacBio sequencing for determination of microbial composition of milk from subclinical mastitis dairy cows of smallholder farms
title_full_unstemmed	Application of culture, PCR, and PacBio sequencing for determination of microbial composition of milk from subclinical mastitis dairy cows of smallholder farms
title_sort	application of culture, pcr, and pacbio sequencing for determination of microbial composition of milk from subclinical mastitis dairy cows of smallholder farms
publisher	De Gruyter
publishDate	2021
url	https://doaj.org/article/ce62630c607247ddad7863fe44cd73e2
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Application of culture, PCR, and PacBio sequencing for determination of microbial composition of milk from subclinical mastitis dairy cows of smallholder farms

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