Insight Into the Beneficial Role of Lactiplantibacillus plantarum Supernatant Against Bacterial Infections, Oxidative Stress, and Wound Healing in A549 Cells and BALB/c Mice

Lactiplantibacillus plantarum MTCC 2621 is a well-characterized probiotic strain and is reported to possess many health benefits. However, the wound healing potential of this probiotic is yet to be explored. Here, we have assessed the antibacterial, antioxidant, and wound healing activities of cell-...

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Autores principales:	Ashish Kumar Dubey, Mansi Podia, Priyanka, Sachin Raut, Sanpreet Singh, Anil Kumar Pinnaka, Neeraj Khatri
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	oxidative stress wound healing inflammation cell-free supernatant A549 cells BALB/c mice Therapeutics. Pharmacology RM1-950
Acceso en línea:	https://doaj.org/article/ce6cfb48fb014ca8b758044cec2a7ef3
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spelling	oai:doaj.org-article:ce6cfb48fb014ca8b758044cec2a7ef32021-11-04T07:30:34ZInsight Into the Beneficial Role of Lactiplantibacillus plantarum Supernatant Against Bacterial Infections, Oxidative Stress, and Wound Healing in A549 Cells and BALB/c Mice1663-981210.3389/fphar.2021.728614https://doaj.org/article/ce6cfb48fb014ca8b758044cec2a7ef32021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fphar.2021.728614/fullhttps://doaj.org/toc/1663-9812Lactiplantibacillus plantarum MTCC 2621 is a well-characterized probiotic strain and is reported to possess many health benefits. However, the wound healing potential of this probiotic is yet to be explored. Here, we have assessed the antibacterial, antioxidant, and wound healing activities of cell-free supernatant of Lactiplantibacillus plantarum MTCC 2621 (Lp2621). Lp2621 exhibited excellent antibacterial activity against the indicator bacteria in the agar well diffusion assay. Lp2621 did not show any hemolytic activity. The safety of Lp2621 gel was established using the skin irritation assay in BALB/c mice, and no dermal reactions were observed. The supernatant showed 60–100% protection of A549 cells against H2O2-induced stress. In the scratch assay, Lp2621 accelerated wound healing after 24 h of treatment. The percent wound healing was significantly higher in cells treated with Lp2621 at 18–24 h posttreatment. In an excision wound healing in mice, topical application of Lp2621 gel showed faster healing than the vehicle- and betadine-treated groups. Similar wound healing activity was observed in wounds infected with Staphylococcus aureus. Histological examination revealed better wound healing in Lp2621-treated mice. Topical treatment of the wounds with Lp2621 gel resulted in the upregulation of pro-inflammatory cytokine IL-6 in the early phase of wound healing and enhanced IL-10 expression in the later phase. These findings unveil a protective role of Lp2621 against bacterial infection, oxidative stress, and wound healing.Ashish Kumar DubeyAshish Kumar DubeyMansi Podia Priyanka PriyankaSachin RautSachin RautSanpreet SinghAnil Kumar PinnakaAnil Kumar PinnakaNeeraj KhatriNeeraj KhatriFrontiers Media S.A.articleoxidative stresswound healinginflammationcell-free supernatantA549 cellsBALB/c miceTherapeutics. PharmacologyRM1-950ENFrontiers in Pharmacology, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	oxidative stress wound healing inflammation cell-free supernatant A549 cells BALB/c mice Therapeutics. Pharmacology RM1-950
spellingShingle	oxidative stress wound healing inflammation cell-free supernatant A549 cells BALB/c mice Therapeutics. Pharmacology RM1-950 Ashish Kumar Dubey Ashish Kumar Dubey Mansi Podia Priyanka Priyanka Sachin Raut Sachin Raut Sanpreet Singh Anil Kumar Pinnaka Anil Kumar Pinnaka Neeraj Khatri Neeraj Khatri Insight Into the Beneficial Role of Lactiplantibacillus plantarum Supernatant Against Bacterial Infections, Oxidative Stress, and Wound Healing in A549 Cells and BALB/c Mice
description	Lactiplantibacillus plantarum MTCC 2621 is a well-characterized probiotic strain and is reported to possess many health benefits. However, the wound healing potential of this probiotic is yet to be explored. Here, we have assessed the antibacterial, antioxidant, and wound healing activities of cell-free supernatant of Lactiplantibacillus plantarum MTCC 2621 (Lp2621). Lp2621 exhibited excellent antibacterial activity against the indicator bacteria in the agar well diffusion assay. Lp2621 did not show any hemolytic activity. The safety of Lp2621 gel was established using the skin irritation assay in BALB/c mice, and no dermal reactions were observed. The supernatant showed 60–100% protection of A549 cells against H2O2-induced stress. In the scratch assay, Lp2621 accelerated wound healing after 24 h of treatment. The percent wound healing was significantly higher in cells treated with Lp2621 at 18–24 h posttreatment. In an excision wound healing in mice, topical application of Lp2621 gel showed faster healing than the vehicle- and betadine-treated groups. Similar wound healing activity was observed in wounds infected with Staphylococcus aureus. Histological examination revealed better wound healing in Lp2621-treated mice. Topical treatment of the wounds with Lp2621 gel resulted in the upregulation of pro-inflammatory cytokine IL-6 in the early phase of wound healing and enhanced IL-10 expression in the later phase. These findings unveil a protective role of Lp2621 against bacterial infection, oxidative stress, and wound healing.
format	article
author	Ashish Kumar Dubey Ashish Kumar Dubey Mansi Podia Priyanka Priyanka Sachin Raut Sachin Raut Sanpreet Singh Anil Kumar Pinnaka Anil Kumar Pinnaka Neeraj Khatri Neeraj Khatri
author_facet	Ashish Kumar Dubey Ashish Kumar Dubey Mansi Podia Priyanka Priyanka Sachin Raut Sachin Raut Sanpreet Singh Anil Kumar Pinnaka Anil Kumar Pinnaka Neeraj Khatri Neeraj Khatri
author_sort	Ashish Kumar Dubey
title	Insight Into the Beneficial Role of Lactiplantibacillus plantarum Supernatant Against Bacterial Infections, Oxidative Stress, and Wound Healing in A549 Cells and BALB/c Mice
title_short	Insight Into the Beneficial Role of Lactiplantibacillus plantarum Supernatant Against Bacterial Infections, Oxidative Stress, and Wound Healing in A549 Cells and BALB/c Mice
title_full	Insight Into the Beneficial Role of Lactiplantibacillus plantarum Supernatant Against Bacterial Infections, Oxidative Stress, and Wound Healing in A549 Cells and BALB/c Mice
title_fullStr	Insight Into the Beneficial Role of Lactiplantibacillus plantarum Supernatant Against Bacterial Infections, Oxidative Stress, and Wound Healing in A549 Cells and BALB/c Mice
title_full_unstemmed	Insight Into the Beneficial Role of Lactiplantibacillus plantarum Supernatant Against Bacterial Infections, Oxidative Stress, and Wound Healing in A549 Cells and BALB/c Mice
title_sort	insight into the beneficial role of lactiplantibacillus plantarum supernatant against bacterial infections, oxidative stress, and wound healing in a549 cells and balb/c mice
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/ce6cfb48fb014ca8b758044cec2a7ef3
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Insight Into the Beneficial Role of Lactiplantibacillus plantarum Supernatant Against Bacterial Infections, Oxidative Stress, and Wound Healing in A549 Cells and BALB/c Mice

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