Drought adaptive prediction in potato (Solanum tuberosum) using in vitro and in vivo approaches

Abstract. Laisina JKJ, Maharijaya A, Sobir, Purwito A. 2021. Drought adaptive prediction in potato (Solanum tuberosum) using in vitro and in vivo approaches. Biodiversitas 22: 537-545. Prediction of drought adaptive potatoes requires selection indicators. This study aimed to determine in vitro selec...

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Autores principales:	JANE KATHRYNE JOLANDA LAISINA, AWANG MAHARIJAYA, SOBIR SOBIR, AGUS PURWITO
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2021
Materias:	drought adaptive in vitro in vivo selection indicator solanum tuberosum Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/ce7700e0a0774f4591e846c0c2393123
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spelling	oai:doaj.org-article:ce7700e0a0774f4591e846c0c23931232021-11-22T00:54:56ZDrought adaptive prediction in potato (Solanum tuberosum) using in vitro and in vivo approaches1412-033X2085-472210.13057/biodiv/d220204https://doaj.org/article/ce7700e0a0774f4591e846c0c23931232021-01-01T00:00:00Zhttps://smujo.id/biodiv/article/view/7139https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Abstract. Laisina JKJ, Maharijaya A, Sobir, Purwito A. 2021. Drought adaptive prediction in potato (Solanum tuberosum) using in vitro and in vivo approaches. Biodiversitas 22: 537-545. Prediction of drought adaptive potatoes requires selection indicators. This study aimed to determine in vitro selection indicators for obtaining drought adaptive potato genotypes in vitro and in vivo. The in vitro study was performed using single-node explants from seven genotypes cultured for six weeks on MS medium added with 0.2 M sorbitol to achieve the research aims. The cultures were partially observed and followed by watering the in vitro tubing medium. For the in vivo study, drought stress was applied for 21 days when the plants reached 25 days after planting (DAP) and for 14 days when the plants reached 50 DAP, then plants were irrigated again until harvesting. The results showed that plant height and root length can be used as selection indicators because they had a relative decrease below 50%, a close relationship and had the same variance with the in vitro number of tubers, in vitro tuber fresh weight and in vivo tuber fresh weight in drought stress at 25 DAP. These characters were determinant characters for the clustering pattern in the heat map cluster analysis. Based on these characters, SSI analysis was also performed and it was found that the PKHT4 was more adaptive than the PKHT6 in vitro, but the two genotypes were classified in the medium adaptive in vivo. In conclusion, plant height and root length were determined as the in vitro selection indicators in predicting drought adaptive genotypes in vivo at the plant age of 25 DAP.JANE KATHRYNE JOLANDA LAISINAAWANG MAHARIJAYASOBIR SOBIRAGUS PURWITOMBI & UNS Soloarticledrought adaptivein vitroin vivoselection indicatorsolanum tuberosumBiology (General)QH301-705.5ENBiodiversitas, Vol 22, Iss 2 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	drought adaptive in vitro in vivo selection indicator solanum tuberosum Biology (General) QH301-705.5
spellingShingle	drought adaptive in vitro in vivo selection indicator solanum tuberosum Biology (General) QH301-705.5 JANE KATHRYNE JOLANDA LAISINA AWANG MAHARIJAYA SOBIR SOBIR AGUS PURWITO Drought adaptive prediction in potato (Solanum tuberosum) using in vitro and in vivo approaches
description	Abstract. Laisina JKJ, Maharijaya A, Sobir, Purwito A. 2021. Drought adaptive prediction in potato (Solanum tuberosum) using in vitro and in vivo approaches. Biodiversitas 22: 537-545. Prediction of drought adaptive potatoes requires selection indicators. This study aimed to determine in vitro selection indicators for obtaining drought adaptive potato genotypes in vitro and in vivo. The in vitro study was performed using single-node explants from seven genotypes cultured for six weeks on MS medium added with 0.2 M sorbitol to achieve the research aims. The cultures were partially observed and followed by watering the in vitro tubing medium. For the in vivo study, drought stress was applied for 21 days when the plants reached 25 days after planting (DAP) and for 14 days when the plants reached 50 DAP, then plants were irrigated again until harvesting. The results showed that plant height and root length can be used as selection indicators because they had a relative decrease below 50%, a close relationship and had the same variance with the in vitro number of tubers, in vitro tuber fresh weight and in vivo tuber fresh weight in drought stress at 25 DAP. These characters were determinant characters for the clustering pattern in the heat map cluster analysis. Based on these characters, SSI analysis was also performed and it was found that the PKHT4 was more adaptive than the PKHT6 in vitro, but the two genotypes were classified in the medium adaptive in vivo. In conclusion, plant height and root length were determined as the in vitro selection indicators in predicting drought adaptive genotypes in vivo at the plant age of 25 DAP.
format	article
author	JANE KATHRYNE JOLANDA LAISINA AWANG MAHARIJAYA SOBIR SOBIR AGUS PURWITO
author_facet	JANE KATHRYNE JOLANDA LAISINA AWANG MAHARIJAYA SOBIR SOBIR AGUS PURWITO
author_sort	JANE KATHRYNE JOLANDA LAISINA
title	Drought adaptive prediction in potato (Solanum tuberosum) using in vitro and in vivo approaches
title_short	Drought adaptive prediction in potato (Solanum tuberosum) using in vitro and in vivo approaches
title_full	Drought adaptive prediction in potato (Solanum tuberosum) using in vitro and in vivo approaches
title_fullStr	Drought adaptive prediction in potato (Solanum tuberosum) using in vitro and in vivo approaches
title_full_unstemmed	Drought adaptive prediction in potato (Solanum tuberosum) using in vitro and in vivo approaches
title_sort	drought adaptive prediction in potato (solanum tuberosum) using in vitro and in vivo approaches
publisher	MBI & UNS Solo
publishDate	2021
url	https://doaj.org/article/ce7700e0a0774f4591e846c0c2393123
work_keys_str_mv	AT janekathrynejolandalaisina droughtadaptivepredictioninpotatosolanumtuberosumusinginvitroandinvivoapproaches AT awangmaharijaya droughtadaptivepredictioninpotatosolanumtuberosumusinginvitroandinvivoapproaches AT sobirsobir droughtadaptivepredictioninpotatosolanumtuberosumusinginvitroandinvivoapproaches AT aguspurwito droughtadaptivepredictioninpotatosolanumtuberosumusinginvitroandinvivoapproaches
_version_	1718418488650891264

Drought adaptive prediction in potato (Solanum tuberosum) using in vitro and in vivo approaches

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