Allele intersection analysis: a novel tool for multi locus sequence assignment in multiply infected hosts.

Wolbachia are wide-spread, endogenous α-Proteobacteria of arthropods and filarial nematodes. 15-75% of all insect species are infected with these endosymbionts that alter their host's reproduction to facilitate their spread. In recent years, many insect species infected with multiple Wolbachia...

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Autores principales:	Wolfgang Arthofer, Markus Riegler, Hannes Schuler, Daniela Schneider, Karl Moder, Wolfgang J Miller, Christian Stauffer
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/ce7a587f861f4c0fb15e862df88beee3
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spelling	oai:doaj.org-article:ce7a587f861f4c0fb15e862df88beee32021-11-18T06:50:10ZAllele intersection analysis: a novel tool for multi locus sequence assignment in multiply infected hosts.1932-620310.1371/journal.pone.0022198https://doaj.org/article/ce7a587f861f4c0fb15e862df88beee32011-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21789233/?tool=EBIhttps://doaj.org/toc/1932-6203Wolbachia are wide-spread, endogenous α-Proteobacteria of arthropods and filarial nematodes. 15-75% of all insect species are infected with these endosymbionts that alter their host's reproduction to facilitate their spread. In recent years, many insect species infected with multiple Wolbachia strains have been identified. As the endosymbionts are not cultivable outside living cells, strain typing relies on molecular methods. A Multi Locus Sequence Typing (MLST) system was established for standardizing Wolbachia strain identification. However, MLST requires hosts to harbour individual and not multiple strains of supergroups without recombination. This study revisits the applicability of the current MLST protocols and introduces Allele Intersection Analysis (AIA) as a novel approach. AIA utilizes natural variations in infection patterns and allows correct strain assignment of MLST alleles in multiply infected host species without the need of artificial strain segregation. AIA identifies pairs of multiply infected individuals that share Wolbachia and differ in only one strain. In such pairs, the shared MLST sequences can be used to assign alleles to distinct strains. Furthermore, AIA is a powerful tool to detect recombination events. The underlying principle of AIA may easily be adopted for MLST approaches in other uncultivable bacterial genera that occur as multiple strain infections and the concept may find application in metagenomic high-throughput parallel sequencing projects.Wolfgang ArthoferMarkus RieglerHannes SchulerDaniela SchneiderKarl ModerWolfgang J MillerChristian StaufferPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 7, p e22198 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Wolfgang Arthofer Markus Riegler Hannes Schuler Daniela Schneider Karl Moder Wolfgang J Miller Christian Stauffer Allele intersection analysis: a novel tool for multi locus sequence assignment in multiply infected hosts.
description	Wolbachia are wide-spread, endogenous α-Proteobacteria of arthropods and filarial nematodes. 15-75% of all insect species are infected with these endosymbionts that alter their host's reproduction to facilitate their spread. In recent years, many insect species infected with multiple Wolbachia strains have been identified. As the endosymbionts are not cultivable outside living cells, strain typing relies on molecular methods. A Multi Locus Sequence Typing (MLST) system was established for standardizing Wolbachia strain identification. However, MLST requires hosts to harbour individual and not multiple strains of supergroups without recombination. This study revisits the applicability of the current MLST protocols and introduces Allele Intersection Analysis (AIA) as a novel approach. AIA utilizes natural variations in infection patterns and allows correct strain assignment of MLST alleles in multiply infected host species without the need of artificial strain segregation. AIA identifies pairs of multiply infected individuals that share Wolbachia and differ in only one strain. In such pairs, the shared MLST sequences can be used to assign alleles to distinct strains. Furthermore, AIA is a powerful tool to detect recombination events. The underlying principle of AIA may easily be adopted for MLST approaches in other uncultivable bacterial genera that occur as multiple strain infections and the concept may find application in metagenomic high-throughput parallel sequencing projects.
format	article
author	Wolfgang Arthofer Markus Riegler Hannes Schuler Daniela Schneider Karl Moder Wolfgang J Miller Christian Stauffer
author_facet	Wolfgang Arthofer Markus Riegler Hannes Schuler Daniela Schneider Karl Moder Wolfgang J Miller Christian Stauffer
author_sort	Wolfgang Arthofer
title	Allele intersection analysis: a novel tool for multi locus sequence assignment in multiply infected hosts.
title_short	Allele intersection analysis: a novel tool for multi locus sequence assignment in multiply infected hosts.
title_full	Allele intersection analysis: a novel tool for multi locus sequence assignment in multiply infected hosts.
title_fullStr	Allele intersection analysis: a novel tool for multi locus sequence assignment in multiply infected hosts.
title_full_unstemmed	Allele intersection analysis: a novel tool for multi locus sequence assignment in multiply infected hosts.
title_sort	allele intersection analysis: a novel tool for multi locus sequence assignment in multiply infected hosts.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/ce7a587f861f4c0fb15e862df88beee3
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Allele intersection analysis: a novel tool for multi locus sequence assignment in multiply infected hosts.

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