RECOMBINANT POLYPEPTIDES BASED ON C5A PEPTIDASE AS POTENTIAL VACCINE COMPONENTS AGAINST GROUP B STREPTOCOCCI

Аbstract. Gene fragments encoding N-terminal and central parts of group B streptococci (GBS) C5a peptidase were cloned in E. coli M15. The appropriate recombinant SCPB1a and SCPB3a polypeptides with molecular masses of, resp., 12.0±0.5 kDa and 11.0±0.5 kDa, were subject to expression and affinity pu...

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Main Authors:	N. V. Duplik, I. V. Koroleva, K. B. Grabovskaya, A. N. Suvorov
Format:	article
Language:	RU
Published:	SPb RAACI 2014
Subjects:	group b streptococci c5a peptidase recombinant polypeptides immunogenicity opsonophagocytosis Immunologic diseases. Allergy RC581-607
Online Access:	https://doaj.org/article/ce7d1775d31a4a5b87e916777d8ff4d1
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spelling	oai:doaj.org-article:ce7d1775d31a4a5b87e916777d8ff4d12021-11-18T08:03:39ZRECOMBINANT POLYPEPTIDES BASED ON C5A PEPTIDASE AS POTENTIAL VACCINE COMPONENTS AGAINST GROUP B STREPTOCOCCI1563-06252313-741X10.15789/1563-0625-2011-1-41-48https://doaj.org/article/ce7d1775d31a4a5b87e916777d8ff4d12014-07-01T00:00:00Zhttps://www.mimmun.ru/mimmun/article/view/281https://doaj.org/toc/1563-0625https://doaj.org/toc/2313-741XАbstract. Gene fragments encoding N-terminal and central parts of group B streptococci (GBS) C5a peptidase were cloned in E. coli M15. The appropriate recombinant SCPB1a and SCPB3a polypeptides with molecular masses of, resp., 12.0±0.5 kDa and 11.0±0.5 kDa, were subject to expression and affinity purification. Both polypeptides induced specific antibodies production upon subcutaneous immunization of mice. A more pronounced immune response was detected with SCPB3a injection. Antimicrobial properties of anti-SCPB1a and anti-SCPB3a antibodies have been investigated in vitro (by opsonophagocytosis test) and in vivo (a model of generalized GBS infection in mice). N-terminal and central segments of C5a peptidase molecule have been shown to contain epitopes inducing humoral immune response with production of class G antibodies that efficiently opsonize GBS, whereas recombinant SCPB1a and SCPB3a polypeptides can be recommended for future implications in development of a polycomponent vaccine against GBS. (Med. Immunol., 2011, vol. 13, N 1, pp 41-48)N. V. DuplikI. V. KorolevaK. B. GrabovskayaA. N. SuvorovSPb RAACIarticlegroup b streptococcic5a peptidaserecombinant polypeptidesimmunogenicityopsonophagocytosisImmunologic diseases. AllergyRC581-607RUMedicinskaâ Immunologiâ, Vol 13, Iss 1, Pp 41-48 (2014)
institution	DOAJ
collection	DOAJ
language	RU
topic	group b streptococci c5a peptidase recombinant polypeptides immunogenicity opsonophagocytosis Immunologic diseases. Allergy RC581-607
spellingShingle	group b streptococci c5a peptidase recombinant polypeptides immunogenicity opsonophagocytosis Immunologic diseases. Allergy RC581-607 N. V. Duplik I. V. Koroleva K. B. Grabovskaya A. N. Suvorov RECOMBINANT POLYPEPTIDES BASED ON C5A PEPTIDASE AS POTENTIAL VACCINE COMPONENTS AGAINST GROUP B STREPTOCOCCI
description	Аbstract. Gene fragments encoding N-terminal and central parts of group B streptococci (GBS) C5a peptidase were cloned in E. coli M15. The appropriate recombinant SCPB1a and SCPB3a polypeptides with molecular masses of, resp., 12.0±0.5 kDa and 11.0±0.5 kDa, were subject to expression and affinity purification. Both polypeptides induced specific antibodies production upon subcutaneous immunization of mice. A more pronounced immune response was detected with SCPB3a injection. Antimicrobial properties of anti-SCPB1a and anti-SCPB3a antibodies have been investigated in vitro (by opsonophagocytosis test) and in vivo (a model of generalized GBS infection in mice). N-terminal and central segments of C5a peptidase molecule have been shown to contain epitopes inducing humoral immune response with production of class G antibodies that efficiently opsonize GBS, whereas recombinant SCPB1a and SCPB3a polypeptides can be recommended for future implications in development of a polycomponent vaccine against GBS. (Med. Immunol., 2011, vol. 13, N 1, pp 41-48)
format	article
author	N. V. Duplik I. V. Koroleva K. B. Grabovskaya A. N. Suvorov
author_facet	N. V. Duplik I. V. Koroleva K. B. Grabovskaya A. N. Suvorov
author_sort	N. V. Duplik
title	RECOMBINANT POLYPEPTIDES BASED ON C5A PEPTIDASE AS POTENTIAL VACCINE COMPONENTS AGAINST GROUP B STREPTOCOCCI
title_short	RECOMBINANT POLYPEPTIDES BASED ON C5A PEPTIDASE AS POTENTIAL VACCINE COMPONENTS AGAINST GROUP B STREPTOCOCCI
title_full	RECOMBINANT POLYPEPTIDES BASED ON C5A PEPTIDASE AS POTENTIAL VACCINE COMPONENTS AGAINST GROUP B STREPTOCOCCI
title_fullStr	RECOMBINANT POLYPEPTIDES BASED ON C5A PEPTIDASE AS POTENTIAL VACCINE COMPONENTS AGAINST GROUP B STREPTOCOCCI
title_full_unstemmed	RECOMBINANT POLYPEPTIDES BASED ON C5A PEPTIDASE AS POTENTIAL VACCINE COMPONENTS AGAINST GROUP B STREPTOCOCCI
title_sort	recombinant polypeptides based on c5a peptidase as potential vaccine components against group b streptococci
publisher	SPb RAACI
publishDate	2014
url	https://doaj.org/article/ce7d1775d31a4a5b87e916777d8ff4d1
work_keys_str_mv	AT nvduplik recombinantpolypeptidesbasedonc5apeptidaseaspotentialvaccinecomponentsagainstgroupbstreptococci AT ivkoroleva recombinantpolypeptidesbasedonc5apeptidaseaspotentialvaccinecomponentsagainstgroupbstreptococci AT kbgrabovskaya recombinantpolypeptidesbasedonc5apeptidaseaspotentialvaccinecomponentsagainstgroupbstreptococci AT ansuvorov recombinantpolypeptidesbasedonc5apeptidaseaspotentialvaccinecomponentsagainstgroupbstreptococci
_version_	1718422569457025024

RECOMBINANT POLYPEPTIDES BASED ON C5A PEPTIDASE AS POTENTIAL VACCINE COMPONENTS AGAINST GROUP B STREPTOCOCCI

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