Dissecting the Machinery That Introduces Disulfide Bonds in <named-content content-type="genus-species">Pseudomonas aeruginosa</named-content>

ABSTRACT Disulfide bond formation is required for the folding of many bacterial virulence factors. However, whereas the Escherichia coli disulfide bond-forming system is well characterized, not much is known on the pathways that oxidatively fold proteins in pathogenic bacteria. Here, we report the d...

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Autores principales:	Isabelle S. Arts, Geneviève Ball, Pauline Leverrier, Steven Garvis, Valérie Nicolaes, Didier Vertommen, Bérengère Ize, Veronica Tamu Dufe, Joris Messens, Romé Voulhoux, Jean-François Collet
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2013
Materias:	Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/ce7eb50e935a4fe1a8372d8e3636f5f9
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spelling	oai:doaj.org-article:ce7eb50e935a4fe1a8372d8e3636f5f92021-11-15T15:42:31ZDissecting the Machinery That Introduces Disulfide Bonds in <named-content content-type="genus-species">Pseudomonas aeruginosa</named-content>10.1128/mBio.00912-132150-7511https://doaj.org/article/ce7eb50e935a4fe1a8372d8e3636f5f92013-12-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mBio.00912-13https://doaj.org/toc/2150-7511ABSTRACT Disulfide bond formation is required for the folding of many bacterial virulence factors. However, whereas the Escherichia coli disulfide bond-forming system is well characterized, not much is known on the pathways that oxidatively fold proteins in pathogenic bacteria. Here, we report the detailed unraveling of the pathway that introduces disulfide bonds in the periplasm of the human pathogen Pseudomonas aeruginosa. The genome of P. aeruginosa uniquely encodes two DsbA proteins (P. aeruginosa DsbA1 [PaDsbA1] and PaDsbA2) and two DsbB proteins (PaDsbB1 and PaDsbB2). We found that PaDsbA1, the primary donor of disulfide bonds to secreted proteins, is maintained oxidized in vivo by both PaDsbB1 and PaDsbB2. In vitro reconstitution of the pathway confirms that both PaDsbB1 and PaDsbB2 shuttle electrons from PaDsbA1 to membrane-bound quinones. Accordingly, deletion of both P. aeruginosa dsbB1 (PadsbB1) and PadsbB2 is required to prevent the folding of several P. aeruginosa virulence factors and to lead to a significant decrease in pathogenicity. Using a high-throughput proteomic approach, we also analyzed the impact of PadsbA1 deletion on the global periplasmic proteome of P. aeruginosa, which allowed us to identify more than 20 new potential substrates of this major oxidoreductase. Finally, we report the biochemical and structural characterization of PaDsbA2, a highly oxidizing oxidoreductase, which seems to be expressed under specific conditions. By fully dissecting the machinery that introduces disulfide bonds in P. aeruginosa, our work opens the way to the design of novel antibacterial molecules able to disarm this pathogen by preventing the proper assembly of its arsenal of virulence factors. IMPORTANCE The human pathogen Pseudomonas aeruginosa causes life-threatening infections in immunodepressed and cystic fibrosis patients. The emergence of P. aeruginosa strains resistant to all of the available antibacterial agents calls for the urgent development of new antibiotics active against this bacterium. The pathogenic power of P. aeruginosa is mediated by an arsenal of extracellular virulence factors, most of which are stabilized by disulfide bonds. Thus, targeting the machinery that introduces disulfide bonds appears to be a promising strategy to combat P. aeruginosa. Here, we unraveled the oxidative protein folding system of P. aeruginosa in full detail. The system uniquely consists of two membrane proteins that generate disulfide bonds de novo to deliver them to P. aeruginosa DsbA1 (PaDsbA1), a soluble oxidoreductase. PaDsbA1 in turn donates disulfide bonds to secreted proteins, including virulence factors. Disruption of the disulfide bond formation machinery dramatically decreases P. aeruginosa virulence, confirming that disulfide formation systems are valid targets for the design of antimicrobial drugs.Isabelle S. ArtsGeneviève BallPauline LeverrierSteven GarvisValérie NicolaesDidier VertommenBérengère IzeVeronica Tamu DufeJoris MessensRomé VoulhouxJean-François ColletAmerican Society for MicrobiologyarticleMicrobiologyQR1-502ENmBio, Vol 4, Iss 6 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Microbiology QR1-502
spellingShingle	Microbiology QR1-502 Isabelle S. Arts Geneviève Ball Pauline Leverrier Steven Garvis Valérie Nicolaes Didier Vertommen Bérengère Ize Veronica Tamu Dufe Joris Messens Romé Voulhoux Jean-François Collet Dissecting the Machinery That Introduces Disulfide Bonds in <named-content content-type="genus-species">Pseudomonas aeruginosa</named-content>
description	ABSTRACT Disulfide bond formation is required for the folding of many bacterial virulence factors. However, whereas the Escherichia coli disulfide bond-forming system is well characterized, not much is known on the pathways that oxidatively fold proteins in pathogenic bacteria. Here, we report the detailed unraveling of the pathway that introduces disulfide bonds in the periplasm of the human pathogen Pseudomonas aeruginosa. The genome of P. aeruginosa uniquely encodes two DsbA proteins (P. aeruginosa DsbA1 [PaDsbA1] and PaDsbA2) and two DsbB proteins (PaDsbB1 and PaDsbB2). We found that PaDsbA1, the primary donor of disulfide bonds to secreted proteins, is maintained oxidized in vivo by both PaDsbB1 and PaDsbB2. In vitro reconstitution of the pathway confirms that both PaDsbB1 and PaDsbB2 shuttle electrons from PaDsbA1 to membrane-bound quinones. Accordingly, deletion of both P. aeruginosa dsbB1 (PadsbB1) and PadsbB2 is required to prevent the folding of several P. aeruginosa virulence factors and to lead to a significant decrease in pathogenicity. Using a high-throughput proteomic approach, we also analyzed the impact of PadsbA1 deletion on the global periplasmic proteome of P. aeruginosa, which allowed us to identify more than 20 new potential substrates of this major oxidoreductase. Finally, we report the biochemical and structural characterization of PaDsbA2, a highly oxidizing oxidoreductase, which seems to be expressed under specific conditions. By fully dissecting the machinery that introduces disulfide bonds in P. aeruginosa, our work opens the way to the design of novel antibacterial molecules able to disarm this pathogen by preventing the proper assembly of its arsenal of virulence factors. IMPORTANCE The human pathogen Pseudomonas aeruginosa causes life-threatening infections in immunodepressed and cystic fibrosis patients. The emergence of P. aeruginosa strains resistant to all of the available antibacterial agents calls for the urgent development of new antibiotics active against this bacterium. The pathogenic power of P. aeruginosa is mediated by an arsenal of extracellular virulence factors, most of which are stabilized by disulfide bonds. Thus, targeting the machinery that introduces disulfide bonds appears to be a promising strategy to combat P. aeruginosa. Here, we unraveled the oxidative protein folding system of P. aeruginosa in full detail. The system uniquely consists of two membrane proteins that generate disulfide bonds de novo to deliver them to P. aeruginosa DsbA1 (PaDsbA1), a soluble oxidoreductase. PaDsbA1 in turn donates disulfide bonds to secreted proteins, including virulence factors. Disruption of the disulfide bond formation machinery dramatically decreases P. aeruginosa virulence, confirming that disulfide formation systems are valid targets for the design of antimicrobial drugs.
format	article
author	Isabelle S. Arts Geneviève Ball Pauline Leverrier Steven Garvis Valérie Nicolaes Didier Vertommen Bérengère Ize Veronica Tamu Dufe Joris Messens Romé Voulhoux Jean-François Collet
author_facet	Isabelle S. Arts Geneviève Ball Pauline Leverrier Steven Garvis Valérie Nicolaes Didier Vertommen Bérengère Ize Veronica Tamu Dufe Joris Messens Romé Voulhoux Jean-François Collet
author_sort	Isabelle S. Arts
title	Dissecting the Machinery That Introduces Disulfide Bonds in <named-content content-type="genus-species">Pseudomonas aeruginosa</named-content>
title_short	Dissecting the Machinery That Introduces Disulfide Bonds in <named-content content-type="genus-species">Pseudomonas aeruginosa</named-content>
title_full	Dissecting the Machinery That Introduces Disulfide Bonds in <named-content content-type="genus-species">Pseudomonas aeruginosa</named-content>
title_fullStr	Dissecting the Machinery That Introduces Disulfide Bonds in <named-content content-type="genus-species">Pseudomonas aeruginosa</named-content>
title_full_unstemmed	Dissecting the Machinery That Introduces Disulfide Bonds in <named-content content-type="genus-species">Pseudomonas aeruginosa</named-content>
title_sort	dissecting the machinery that introduces disulfide bonds in <named-content content-type="genus-species">pseudomonas aeruginosa</named-content>
publisher	American Society for Microbiology
publishDate	2013
url	https://doaj.org/article/ce7eb50e935a4fe1a8372d8e3636f5f9
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Dissecting the Machinery That Introduces Disulfide Bonds in <named-content content-type="genus-species">Pseudomonas aeruginosa</named-content>

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