The Abi-domain protein Abx1 interacts with the CovS histidine kinase to control virulence gene expression in group B Streptococcus.

Group B Streptococcus (GBS), a common commensal of the female genital tract, is the leading cause of invasive infections in neonates. Expression of major GBS virulence factors, such as the hemolysin operon cyl, is regulated directly at the transcriptional level by the CovSR two-component system. Usi...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Arnaud Firon, Asmaa Tazi, Violette Da Cunha, Sophie Brinster, Elisabeth Sauvage, Shaynoor Dramsi, Douglas T Golenbock, Philippe Glaser, Claire Poyart, Patrick Trieu-Cuot
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/ce80e8fb70d14066acbdafaf34deea62
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:ce80e8fb70d14066acbdafaf34deea62
record_format	dspace
spelling	oai:doaj.org-article:ce80e8fb70d14066acbdafaf34deea622021-11-18T06:06:01ZThe Abi-domain protein Abx1 interacts with the CovS histidine kinase to control virulence gene expression in group B Streptococcus.1553-73661553-737410.1371/journal.ppat.1003179https://doaj.org/article/ce80e8fb70d14066acbdafaf34deea622013-02-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23436996/pdf/?tool=EBIhttps://doaj.org/toc/1553-7366https://doaj.org/toc/1553-7374Group B Streptococcus (GBS), a common commensal of the female genital tract, is the leading cause of invasive infections in neonates. Expression of major GBS virulence factors, such as the hemolysin operon cyl, is regulated directly at the transcriptional level by the CovSR two-component system. Using a random genetic approach, we identified a multi-spanning transmembrane protein, Abx1, essential for the production of the GBS hemolysin. Despite its similarity to eukaryotic CaaX proteases, the Abx1 function is not involved in a post-translational modification of the GBS hemolysin. Instead, we demonstrate that Abx1 regulates transcription of several virulence genes, including those comprising the hemolysin operon, by a CovSR-dependent mechanism. By combining genetic analyses, transcriptome profiling, and site-directed mutagenesis, we showed that Abx1 is a regulator of the histidine kinase CovS. Overexpression of Abx1 is sufficient to activate virulence gene expression through CovS, overcoming the need for an additional signal. Conversely, the absence of Abx1 has the opposite effect on virulence gene expression consistent with CovS locked in a kinase-competent state. Using a bacterial two-hybrid system, direct interaction between Abx1 and CovS was mapped specifically to CovS domains involved in signal processing. We demonstrate that the CovSR two-component system is the core of a signaling pathway integrating the regulation of CovS by Abx1 in addition to the regulation of CovR by the serine/threonine kinase Stk1. In conclusion, our study reports a regulatory function for Abx1, a member of a large protein family with a characteristic Abi-domain, which forms a signaling complex with the histidine kinase CovS in GBS.Arnaud FironAsmaa TaziViolette Da CunhaSophie BrinsterElisabeth SauvageShaynoor DramsiDouglas T GolenbockPhilippe GlaserClaire PoyartPatrick Trieu-CuotPublic Library of Science (PLoS)articleImmunologic diseases. AllergyRC581-607Biology (General)QH301-705.5ENPLoS Pathogens, Vol 9, Iss 2, p e1003179 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5
spellingShingle	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5 Arnaud Firon Asmaa Tazi Violette Da Cunha Sophie Brinster Elisabeth Sauvage Shaynoor Dramsi Douglas T Golenbock Philippe Glaser Claire Poyart Patrick Trieu-Cuot The Abi-domain protein Abx1 interacts with the CovS histidine kinase to control virulence gene expression in group B Streptococcus.
description	Group B Streptococcus (GBS), a common commensal of the female genital tract, is the leading cause of invasive infections in neonates. Expression of major GBS virulence factors, such as the hemolysin operon cyl, is regulated directly at the transcriptional level by the CovSR two-component system. Using a random genetic approach, we identified a multi-spanning transmembrane protein, Abx1, essential for the production of the GBS hemolysin. Despite its similarity to eukaryotic CaaX proteases, the Abx1 function is not involved in a post-translational modification of the GBS hemolysin. Instead, we demonstrate that Abx1 regulates transcription of several virulence genes, including those comprising the hemolysin operon, by a CovSR-dependent mechanism. By combining genetic analyses, transcriptome profiling, and site-directed mutagenesis, we showed that Abx1 is a regulator of the histidine kinase CovS. Overexpression of Abx1 is sufficient to activate virulence gene expression through CovS, overcoming the need for an additional signal. Conversely, the absence of Abx1 has the opposite effect on virulence gene expression consistent with CovS locked in a kinase-competent state. Using a bacterial two-hybrid system, direct interaction between Abx1 and CovS was mapped specifically to CovS domains involved in signal processing. We demonstrate that the CovSR two-component system is the core of a signaling pathway integrating the regulation of CovS by Abx1 in addition to the regulation of CovR by the serine/threonine kinase Stk1. In conclusion, our study reports a regulatory function for Abx1, a member of a large protein family with a characteristic Abi-domain, which forms a signaling complex with the histidine kinase CovS in GBS.
format	article
author	Arnaud Firon Asmaa Tazi Violette Da Cunha Sophie Brinster Elisabeth Sauvage Shaynoor Dramsi Douglas T Golenbock Philippe Glaser Claire Poyart Patrick Trieu-Cuot
author_facet	Arnaud Firon Asmaa Tazi Violette Da Cunha Sophie Brinster Elisabeth Sauvage Shaynoor Dramsi Douglas T Golenbock Philippe Glaser Claire Poyart Patrick Trieu-Cuot
author_sort	Arnaud Firon
title	The Abi-domain protein Abx1 interacts with the CovS histidine kinase to control virulence gene expression in group B Streptococcus.
title_short	The Abi-domain protein Abx1 interacts with the CovS histidine kinase to control virulence gene expression in group B Streptococcus.
title_full	The Abi-domain protein Abx1 interacts with the CovS histidine kinase to control virulence gene expression in group B Streptococcus.
title_fullStr	The Abi-domain protein Abx1 interacts with the CovS histidine kinase to control virulence gene expression in group B Streptococcus.
title_full_unstemmed	The Abi-domain protein Abx1 interacts with the CovS histidine kinase to control virulence gene expression in group B Streptococcus.
title_sort	abi-domain protein abx1 interacts with the covs histidine kinase to control virulence gene expression in group b streptococcus.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/ce80e8fb70d14066acbdafaf34deea62
work_keys_str_mv	AT arnaudfiron theabidomainproteinabx1interactswiththecovshistidinekinasetocontrolvirulencegeneexpressioningroupbstreptococcus AT asmaatazi theabidomainproteinabx1interactswiththecovshistidinekinasetocontrolvirulencegeneexpressioningroupbstreptococcus AT violettedacunha theabidomainproteinabx1interactswiththecovshistidinekinasetocontrolvirulencegeneexpressioningroupbstreptococcus AT sophiebrinster theabidomainproteinabx1interactswiththecovshistidinekinasetocontrolvirulencegeneexpressioningroupbstreptococcus AT elisabethsauvage theabidomainproteinabx1interactswiththecovshistidinekinasetocontrolvirulencegeneexpressioningroupbstreptococcus AT shaynoordramsi theabidomainproteinabx1interactswiththecovshistidinekinasetocontrolvirulencegeneexpressioningroupbstreptococcus AT douglastgolenbock theabidomainproteinabx1interactswiththecovshistidinekinasetocontrolvirulencegeneexpressioningroupbstreptococcus AT philippeglaser theabidomainproteinabx1interactswiththecovshistidinekinasetocontrolvirulencegeneexpressioningroupbstreptococcus AT clairepoyart theabidomainproteinabx1interactswiththecovshistidinekinasetocontrolvirulencegeneexpressioningroupbstreptococcus AT patricktrieucuot theabidomainproteinabx1interactswiththecovshistidinekinasetocontrolvirulencegeneexpressioningroupbstreptococcus AT arnaudfiron abidomainproteinabx1interactswiththecovshistidinekinasetocontrolvirulencegeneexpressioningroupbstreptococcus AT asmaatazi abidomainproteinabx1interactswiththecovshistidinekinasetocontrolvirulencegeneexpressioningroupbstreptococcus AT violettedacunha abidomainproteinabx1interactswiththecovshistidinekinasetocontrolvirulencegeneexpressioningroupbstreptococcus AT sophiebrinster abidomainproteinabx1interactswiththecovshistidinekinasetocontrolvirulencegeneexpressioningroupbstreptococcus AT elisabethsauvage abidomainproteinabx1interactswiththecovshistidinekinasetocontrolvirulencegeneexpressioningroupbstreptococcus AT shaynoordramsi abidomainproteinabx1interactswiththecovshistidinekinasetocontrolvirulencegeneexpressioningroupbstreptococcus AT douglastgolenbock abidomainproteinabx1interactswiththecovshistidinekinasetocontrolvirulencegeneexpressioningroupbstreptococcus AT philippeglaser abidomainproteinabx1interactswiththecovshistidinekinasetocontrolvirulencegeneexpressioningroupbstreptococcus AT clairepoyart abidomainproteinabx1interactswiththecovshistidinekinasetocontrolvirulencegeneexpressioningroupbstreptococcus AT patricktrieucuot abidomainproteinabx1interactswiththecovshistidinekinasetocontrolvirulencegeneexpressioningroupbstreptococcus
_version_	1718424568018763776

The Abi-domain protein Abx1 interacts with the CovS histidine kinase to control virulence gene expression in group B Streptococcus.

Ejemplares similares