miR-877-3p inhibits tumor growth and angiogenesis of osteosarcoma through Fibroblast Growth Factor 2 signaling

Osteosarcoma (OS) is the most common high-grade malignant bone tumor in teenagers. MicroRNAs can function as post-transcriptional regulators of gene expression, playing critical roles in cancer development and metastasis. Our study aims to investigate the role and underlying molecular mechanism of m...

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Autores principales:	Hailin Zhou, Lei Cao, Cheng Wang, Chi Fang, HaiHui Wu, Chao Liu
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2021
Materias:	mir-877-3p osteosarcoma proliferation angiogenesis fgf2 Biotechnology TP248.13-248.65
Acceso en línea:	https://doaj.org/article/ce8dd4746e864a98a24cad4a153d214b
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spelling	oai:doaj.org-article:ce8dd4746e864a98a24cad4a153d214b2021-11-11T14:23:43ZmiR-877-3p inhibits tumor growth and angiogenesis of osteosarcoma through Fibroblast Growth Factor 2 signaling2165-59792165-598710.1080/21655979.2021.1982305https://doaj.org/article/ce8dd4746e864a98a24cad4a153d214b2021-09-01T00:00:00Zhttp://dx.doi.org/10.1080/21655979.2021.1982305https://doaj.org/toc/2165-5979https://doaj.org/toc/2165-5987Osteosarcoma (OS) is the most common high-grade malignant bone tumor in teenagers. MicroRNAs can function as post-transcriptional regulators of gene expression, playing critical roles in cancer development and metastasis. Our study aims to investigate the role and underlying molecular mechanism of miR-877-3p in OS. Quantitative real-time RT-PCR was carried out for detecting miR-877-3p expression in OS. The effects of miR-877-3p on proliferation was analyzed via MTT, colony formation and flow cytometry assays. Angiogenesis of endothelial cells were investigated by wound healing and tube formation assay. Gene profiling based on PCR array and luciferase reporter assay were conducted to determine target genes of miR-877-3p. In-vivo study was used to determine the effects of miR-877-3p on the tumor growth. The expression of miR-877-3p was markedly down-regulated in OS tissues and cell lines. Low expression of miR-877-3p predicts poor prognosis of OS patients. miR877-3p overexpression was found to inhibit the proliferation of OS cell lines. The angiogenesis assays showed that miR-877-3p attenuated the angiogenesis. Further mechanism studies showed that miR-877-3p can reduce (Fibroblast Growth Factor 2) FGF2 expression in OS cells by binding to the 3ʹUTR end of FGF2. Moreover, increased expression of miR-877-3p was responsible for the inhibition of tumor growth and angiogenesis. Taken together, our findings indicated that miR-877-3p might exhibit tumor suppressive role by targeting FGF2 signaling, which may serve as potential target for OS.Hailin ZhouLei CaoCheng WangChi FangHaiHui WuChao LiuTaylor & Francis Grouparticlemir-877-3posteosarcomaproliferationangiogenesisfgf2BiotechnologyTP248.13-248.65ENBioengineered, Vol 0, Iss 0 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	mir-877-3p osteosarcoma proliferation angiogenesis fgf2 Biotechnology TP248.13-248.65
spellingShingle	mir-877-3p osteosarcoma proliferation angiogenesis fgf2 Biotechnology TP248.13-248.65 Hailin Zhou Lei Cao Cheng Wang Chi Fang HaiHui Wu Chao Liu miR-877-3p inhibits tumor growth and angiogenesis of osteosarcoma through Fibroblast Growth Factor 2 signaling
description	Osteosarcoma (OS) is the most common high-grade malignant bone tumor in teenagers. MicroRNAs can function as post-transcriptional regulators of gene expression, playing critical roles in cancer development and metastasis. Our study aims to investigate the role and underlying molecular mechanism of miR-877-3p in OS. Quantitative real-time RT-PCR was carried out for detecting miR-877-3p expression in OS. The effects of miR-877-3p on proliferation was analyzed via MTT, colony formation and flow cytometry assays. Angiogenesis of endothelial cells were investigated by wound healing and tube formation assay. Gene profiling based on PCR array and luciferase reporter assay were conducted to determine target genes of miR-877-3p. In-vivo study was used to determine the effects of miR-877-3p on the tumor growth. The expression of miR-877-3p was markedly down-regulated in OS tissues and cell lines. Low expression of miR-877-3p predicts poor prognosis of OS patients. miR877-3p overexpression was found to inhibit the proliferation of OS cell lines. The angiogenesis assays showed that miR-877-3p attenuated the angiogenesis. Further mechanism studies showed that miR-877-3p can reduce (Fibroblast Growth Factor 2) FGF2 expression in OS cells by binding to the 3ʹUTR end of FGF2. Moreover, increased expression of miR-877-3p was responsible for the inhibition of tumor growth and angiogenesis. Taken together, our findings indicated that miR-877-3p might exhibit tumor suppressive role by targeting FGF2 signaling, which may serve as potential target for OS.
format	article
author	Hailin Zhou Lei Cao Cheng Wang Chi Fang HaiHui Wu Chao Liu
author_facet	Hailin Zhou Lei Cao Cheng Wang Chi Fang HaiHui Wu Chao Liu
author_sort	Hailin Zhou
title	miR-877-3p inhibits tumor growth and angiogenesis of osteosarcoma through Fibroblast Growth Factor 2 signaling
title_short	miR-877-3p inhibits tumor growth and angiogenesis of osteosarcoma through Fibroblast Growth Factor 2 signaling
title_full	miR-877-3p inhibits tumor growth and angiogenesis of osteosarcoma through Fibroblast Growth Factor 2 signaling
title_fullStr	miR-877-3p inhibits tumor growth and angiogenesis of osteosarcoma through Fibroblast Growth Factor 2 signaling
title_full_unstemmed	miR-877-3p inhibits tumor growth and angiogenesis of osteosarcoma through Fibroblast Growth Factor 2 signaling
title_sort	mir-877-3p inhibits tumor growth and angiogenesis of osteosarcoma through fibroblast growth factor 2 signaling
publisher	Taylor & Francis Group
publishDate	2021
url	https://doaj.org/article/ce8dd4746e864a98a24cad4a153d214b
work_keys_str_mv	AT hailinzhou mir8773pinhibitstumorgrowthandangiogenesisofosteosarcomathroughfibroblastgrowthfactor2signaling AT leicao mir8773pinhibitstumorgrowthandangiogenesisofosteosarcomathroughfibroblastgrowthfactor2signaling AT chengwang mir8773pinhibitstumorgrowthandangiogenesisofosteosarcomathroughfibroblastgrowthfactor2signaling AT chifang mir8773pinhibitstumorgrowthandangiogenesisofosteosarcomathroughfibroblastgrowthfactor2signaling AT haihuiwu mir8773pinhibitstumorgrowthandangiogenesisofosteosarcomathroughfibroblastgrowthfactor2signaling AT chaoliu mir8773pinhibitstumorgrowthandangiogenesisofosteosarcomathroughfibroblastgrowthfactor2signaling
_version_	1718438987110023168

miR-877-3p inhibits tumor growth and angiogenesis of osteosarcoma through Fibroblast Growth Factor 2 signaling

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