Antiviral Effect of Lithium Chloride on Replication of Marek’s Disease Virus in Chicken Embryonic Fibroblasts

To investigate the antiviral effect of lithium chloride (LiCl) on the replication of Marek’s disease virus (MDV) in chicken embryonic fibroblast (CEF) cells, real-time PCR, Western blotting, plaque counting, and indirect immunofluorescence experiments were performed at different time points of LiCl...

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Autores principales:	Huifeng He, Dandan Qiao, Lu Zhang, Yongxiu Yao, Hongxia Shao, Aijian Qin, Kun Qian
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	Marek’s disease virus lithium chloride virus replication inhibition Biology (General) QH301-705.5 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/ce8f95f3c3e5406c96f4a8cd4053102f
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spelling	oai:doaj.org-article:ce8f95f3c3e5406c96f4a8cd4053102f2021-11-25T17:55:54ZAntiviral Effect of Lithium Chloride on Replication of Marek’s Disease Virus in Chicken Embryonic Fibroblasts10.3390/ijms2222123751422-00671661-6596https://doaj.org/article/ce8f95f3c3e5406c96f4a8cd4053102f2021-11-01T00:00:00Zhttps://www.mdpi.com/1422-0067/22/22/12375https://doaj.org/toc/1661-6596https://doaj.org/toc/1422-0067To investigate the antiviral effect of lithium chloride (LiCl) on the replication of Marek’s disease virus (MDV) in chicken embryonic fibroblast (CEF) cells, real-time PCR, Western blotting, plaque counting, and indirect immunofluorescence experiments were performed at different time points of LiCl treated CEF cells with virus infection. The results demonstrated that LiCl could affect multiple steps of virus replication and inhibit viral gene expression and protein synthesis in a dose- and time-dependent manner. Moreover, LiCl could directly affect viral infectivity as well. In addition, LiCl significantly affected the gene expression of IFN-β related genes in virus-infected cells. These results indicate that LiCl significantly inhibits MDV replication and proliferation in CEF cells and it has the potential to be used as an antiviral agent against MDV.Huifeng HeDandan QiaoLu ZhangYongxiu YaoHongxia ShaoAijian QinKun QianMDPI AGarticleMarek’s disease viruslithium chloridevirus replicationinhibitionBiology (General)QH301-705.5ChemistryQD1-999ENInternational Journal of Molecular Sciences, Vol 22, Iss 12375, p 12375 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Marek’s disease virus lithium chloride virus replication inhibition Biology (General) QH301-705.5 Chemistry QD1-999
spellingShingle	Marek’s disease virus lithium chloride virus replication inhibition Biology (General) QH301-705.5 Chemistry QD1-999 Huifeng He Dandan Qiao Lu Zhang Yongxiu Yao Hongxia Shao Aijian Qin Kun Qian Antiviral Effect of Lithium Chloride on Replication of Marek’s Disease Virus in Chicken Embryonic Fibroblasts
description	To investigate the antiviral effect of lithium chloride (LiCl) on the replication of Marek’s disease virus (MDV) in chicken embryonic fibroblast (CEF) cells, real-time PCR, Western blotting, plaque counting, and indirect immunofluorescence experiments were performed at different time points of LiCl treated CEF cells with virus infection. The results demonstrated that LiCl could affect multiple steps of virus replication and inhibit viral gene expression and protein synthesis in a dose- and time-dependent manner. Moreover, LiCl could directly affect viral infectivity as well. In addition, LiCl significantly affected the gene expression of IFN-β related genes in virus-infected cells. These results indicate that LiCl significantly inhibits MDV replication and proliferation in CEF cells and it has the potential to be used as an antiviral agent against MDV.
format	article
author	Huifeng He Dandan Qiao Lu Zhang Yongxiu Yao Hongxia Shao Aijian Qin Kun Qian
author_facet	Huifeng He Dandan Qiao Lu Zhang Yongxiu Yao Hongxia Shao Aijian Qin Kun Qian
author_sort	Huifeng He
title	Antiviral Effect of Lithium Chloride on Replication of Marek’s Disease Virus in Chicken Embryonic Fibroblasts
title_short	Antiviral Effect of Lithium Chloride on Replication of Marek’s Disease Virus in Chicken Embryonic Fibroblasts
title_full	Antiviral Effect of Lithium Chloride on Replication of Marek’s Disease Virus in Chicken Embryonic Fibroblasts
title_fullStr	Antiviral Effect of Lithium Chloride on Replication of Marek’s Disease Virus in Chicken Embryonic Fibroblasts
title_full_unstemmed	Antiviral Effect of Lithium Chloride on Replication of Marek’s Disease Virus in Chicken Embryonic Fibroblasts
title_sort	antiviral effect of lithium chloride on replication of marek’s disease virus in chicken embryonic fibroblasts
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/ce8f95f3c3e5406c96f4a8cd4053102f
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_version_	1718411831068852224

Antiviral Effect of Lithium Chloride on Replication of Marek’s Disease Virus in Chicken Embryonic Fibroblasts

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