EXPRESSION OF A NEW A3 ANTIGEN IN THE CELLS OF PATIENTS WITH VARIOUS LYMPHOPROLIFERATIVE DISEASES

We have conducted a study of a novel monoclonal A3 antibody raised by means of hybridoma biotechnology. The study was performed with malignant cells of the patients with various lymphoproliferative disorders, and persons with nonmalignant diseases, as compared with intact lymphocytes from healthy pe...

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Autores principales:	N. L. Deineko, T. I. Bulycheva, A. M. Kovrigina, A. A. Grigoriyv
Formato:	article
Lenguaje:	RU
Publicado:	SPb RAACI 2014
Materias:	antigen a3 lymphoproliferative diseases nucleoli immunocytochemistry Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/ce932ee077a94ad58176c23dd09c4f50
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spelling	oai:doaj.org-article:ce932ee077a94ad58176c23dd09c4f502021-11-18T08:03:44ZEXPRESSION OF A NEW A3 ANTIGEN IN THE CELLS OF PATIENTS WITH VARIOUS LYMPHOPROLIFERATIVE DISEASES1563-06252313-741X10.15789/1563-0625-2014-5-437-442https://doaj.org/article/ce932ee077a94ad58176c23dd09c4f502014-11-01T00:00:00Zhttps://www.mimmun.ru/mimmun/article/view/730https://doaj.org/toc/1563-0625https://doaj.org/toc/2313-741XWe have conducted a study of a novel monoclonal A3 antibody raised by means of hybridoma biotechnology. The study was performed with malignant cells of the patients with various lymphoproliferative disorders, and persons with nonmalignant diseases, as compared with intact lymphocytes from healthy people,using a method of immunocytochemical staining and indirect immunofluorescence technique. It was found that in cases of lymphoproliferative diseases with low proliferation rates, as based on the numbers of Ki-67 positive cells, as well as in non-malignant blood diseases, the A3 antigen was localized in nucleoli, and it was visualized as focal fluorescence. In malignant lymphoproliferative diseases with high proliferation indexes, the number of brightly fluorescent foci is observed, with formation of necklace-like structures within the nucleolar structures. The obtained data point to a diagnostic significance of A3 Mab in assessment of cellular proliferative rates in patients with various lymphoproliferative diseases. It was established that, in contrast to Ki-67, the proliferation stage could be determined for each cell, according to the number of fuorescent foci in nucleoli. This specific property of the A3 antigen points to its significance for diagnostics and malignancy staging of lymphoproliferative disorders.N. L. DeinekoT. I. BulychevaA. M. KovriginaA. A. GrigoriyvSPb RAACIarticleantigen a3lymphoproliferative diseasesnucleoliimmunocytochemistryImmunologic diseases. AllergyRC581-607RUMedicinskaâ Immunologiâ, Vol 16, Iss 5, Pp 437-442 (2014)
institution	DOAJ
collection	DOAJ
language	RU
topic	antigen a3 lymphoproliferative diseases nucleoli immunocytochemistry Immunologic diseases. Allergy RC581-607
spellingShingle	antigen a3 lymphoproliferative diseases nucleoli immunocytochemistry Immunologic diseases. Allergy RC581-607 N. L. Deineko T. I. Bulycheva A. M. Kovrigina A. A. Grigoriyv EXPRESSION OF A NEW A3 ANTIGEN IN THE CELLS OF PATIENTS WITH VARIOUS LYMPHOPROLIFERATIVE DISEASES
description	We have conducted a study of a novel monoclonal A3 antibody raised by means of hybridoma biotechnology. The study was performed with malignant cells of the patients with various lymphoproliferative disorders, and persons with nonmalignant diseases, as compared with intact lymphocytes from healthy people,using a method of immunocytochemical staining and indirect immunofluorescence technique. It was found that in cases of lymphoproliferative diseases with low proliferation rates, as based on the numbers of Ki-67 positive cells, as well as in non-malignant blood diseases, the A3 antigen was localized in nucleoli, and it was visualized as focal fluorescence. In malignant lymphoproliferative diseases with high proliferation indexes, the number of brightly fluorescent foci is observed, with formation of necklace-like structures within the nucleolar structures. The obtained data point to a diagnostic significance of A3 Mab in assessment of cellular proliferative rates in patients with various lymphoproliferative diseases. It was established that, in contrast to Ki-67, the proliferation stage could be determined for each cell, according to the number of fuorescent foci in nucleoli. This specific property of the A3 antigen points to its significance for diagnostics and malignancy staging of lymphoproliferative disorders.
format	article
author	N. L. Deineko T. I. Bulycheva A. M. Kovrigina A. A. Grigoriyv
author_facet	N. L. Deineko T. I. Bulycheva A. M. Kovrigina A. A. Grigoriyv
author_sort	N. L. Deineko
title	EXPRESSION OF A NEW A3 ANTIGEN IN THE CELLS OF PATIENTS WITH VARIOUS LYMPHOPROLIFERATIVE DISEASES
title_short	EXPRESSION OF A NEW A3 ANTIGEN IN THE CELLS OF PATIENTS WITH VARIOUS LYMPHOPROLIFERATIVE DISEASES
title_full	EXPRESSION OF A NEW A3 ANTIGEN IN THE CELLS OF PATIENTS WITH VARIOUS LYMPHOPROLIFERATIVE DISEASES
title_fullStr	EXPRESSION OF A NEW A3 ANTIGEN IN THE CELLS OF PATIENTS WITH VARIOUS LYMPHOPROLIFERATIVE DISEASES
title_full_unstemmed	EXPRESSION OF A NEW A3 ANTIGEN IN THE CELLS OF PATIENTS WITH VARIOUS LYMPHOPROLIFERATIVE DISEASES
title_sort	expression of a new a3 antigen in the cells of patients with various lymphoproliferative diseases
publisher	SPb RAACI
publishDate	2014
url	https://doaj.org/article/ce932ee077a94ad58176c23dd09c4f50
work_keys_str_mv	AT nldeineko expressionofanewa3antigeninthecellsofpatientswithvariouslymphoproliferativediseases AT tibulycheva expressionofanewa3antigeninthecellsofpatientswithvariouslymphoproliferativediseases AT amkovrigina expressionofanewa3antigeninthecellsofpatientswithvariouslymphoproliferativediseases AT aagrigoriyv expressionofanewa3antigeninthecellsofpatientswithvariouslymphoproliferativediseases
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EXPRESSION OF A NEW A3 ANTIGEN IN THE CELLS OF PATIENTS WITH VARIOUS LYMPHOPROLIFERATIVE DISEASES

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