Ex-Vivo Force Spectroscopy of Intestinal Mucosa Reveals the Mechanical Properties of Mucus Blankets

Abstract Mucus is the viscous gel that protects mucosal surfaces. It also plays a crucial role in several diseases as well as in mucosal drug delivery. Because of technical limitations, mucus properties have mainly been addressed by in-vitro studies. However, this approach can lead to artifacts as m...

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Autores principales:	Javier Sotres, Skaidre Jankovskaja, Kristin Wannerberger, Thomas Arnebrant
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/ce97437174cd41519b344ea528126bcf
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spelling	oai:doaj.org-article:ce97437174cd41519b344ea528126bcf2021-12-02T15:06:26ZEx-Vivo Force Spectroscopy of Intestinal Mucosa Reveals the Mechanical Properties of Mucus Blankets10.1038/s41598-017-07552-72045-2322https://doaj.org/article/ce97437174cd41519b344ea528126bcf2017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-07552-7https://doaj.org/toc/2045-2322Abstract Mucus is the viscous gel that protects mucosal surfaces. It also plays a crucial role in several diseases as well as in mucosal drug delivery. Because of technical limitations, mucus properties have mainly been addressed by in-vitro studies. However, this approach can lead to artifacts as mucus collection can alter its structure. Here we show that by using an implemented atomic force microscope it is possible to measure the interactions between micro-particles and mucus blankets ex-vivo i.e., on fresh excised mucus-covered tissues. By applying this method to study the small intestine, we were able to quantify the stiffness and adhesiveness of its mucus blanket at different pH values. We also demonstrate the ability of mucus blankets to bind and attract particles hundreds of µm away from their surface, and to trap and bury them even if their size is as big as 15 µm.Javier SotresSkaidre JankovskajaKristin WannerbergerThomas ArnebrantNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-14 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Javier Sotres Skaidre Jankovskaja Kristin Wannerberger Thomas Arnebrant Ex-Vivo Force Spectroscopy of Intestinal Mucosa Reveals the Mechanical Properties of Mucus Blankets
description	Abstract Mucus is the viscous gel that protects mucosal surfaces. It also plays a crucial role in several diseases as well as in mucosal drug delivery. Because of technical limitations, mucus properties have mainly been addressed by in-vitro studies. However, this approach can lead to artifacts as mucus collection can alter its structure. Here we show that by using an implemented atomic force microscope it is possible to measure the interactions between micro-particles and mucus blankets ex-vivo i.e., on fresh excised mucus-covered tissues. By applying this method to study the small intestine, we were able to quantify the stiffness and adhesiveness of its mucus blanket at different pH values. We also demonstrate the ability of mucus blankets to bind and attract particles hundreds of µm away from their surface, and to trap and bury them even if their size is as big as 15 µm.
format	article
author	Javier Sotres Skaidre Jankovskaja Kristin Wannerberger Thomas Arnebrant
author_facet	Javier Sotres Skaidre Jankovskaja Kristin Wannerberger Thomas Arnebrant
author_sort	Javier Sotres
title	Ex-Vivo Force Spectroscopy of Intestinal Mucosa Reveals the Mechanical Properties of Mucus Blankets
title_short	Ex-Vivo Force Spectroscopy of Intestinal Mucosa Reveals the Mechanical Properties of Mucus Blankets
title_full	Ex-Vivo Force Spectroscopy of Intestinal Mucosa Reveals the Mechanical Properties of Mucus Blankets
title_fullStr	Ex-Vivo Force Spectroscopy of Intestinal Mucosa Reveals the Mechanical Properties of Mucus Blankets
title_full_unstemmed	Ex-Vivo Force Spectroscopy of Intestinal Mucosa Reveals the Mechanical Properties of Mucus Blankets
title_sort	ex-vivo force spectroscopy of intestinal mucosa reveals the mechanical properties of mucus blankets
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/ce97437174cd41519b344ea528126bcf
work_keys_str_mv	AT javiersotres exvivoforcespectroscopyofintestinalmucosarevealsthemechanicalpropertiesofmucusblankets AT skaidrejankovskaja exvivoforcespectroscopyofintestinalmucosarevealsthemechanicalpropertiesofmucusblankets AT kristinwannerberger exvivoforcespectroscopyofintestinalmucosarevealsthemechanicalpropertiesofmucusblankets AT thomasarnebrant exvivoforcespectroscopyofintestinalmucosarevealsthemechanicalpropertiesofmucusblankets
_version_	1718388480411697152

Ex-Vivo Force Spectroscopy of Intestinal Mucosa Reveals the Mechanical Properties of Mucus Blankets

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