Biosensor based on tyrosinase immobilized on a single-walled carbon nanotube-modified glassy carbon electrode for detection of epinephrine

Irina Mirela Apetrei,1 Constantin Apetrei21Department of Pharmaceutical Sciences, Faculty of Medicine and Pharmacy, 2Department of Chemistry, Physics and Environment, Faculty of Sciences and Environment, Dunarea de Jos University of Galati, RomaniaAbstract: A biosensor comprising tyrosinase immobili...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Apetrei IM, Apetrei C
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2013
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/ce9f6b9ad6ee49209d1499c967cf1783
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:ce9f6b9ad6ee49209d1499c967cf1783
record_format	dspace
spelling	oai:doaj.org-article:ce9f6b9ad6ee49209d1499c967cf17832021-12-02T01:13:55ZBiosensor based on tyrosinase immobilized on a single-walled carbon nanotube-modified glassy carbon electrode for detection of epinephrine1176-91141178-2013https://doaj.org/article/ce9f6b9ad6ee49209d1499c967cf17832013-11-01T00:00:00Zhttp://www.dovepress.com/biosensor-based-on-tyrosinase-immobilized-on-a-single-walled-carbon-na-a14968https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Irina Mirela Apetrei,1 Constantin Apetrei21Department of Pharmaceutical Sciences, Faculty of Medicine and Pharmacy, 2Department of Chemistry, Physics and Environment, Faculty of Sciences and Environment, Dunarea de Jos University of Galati, RomaniaAbstract: A biosensor comprising tyrosinase immobilized on a single-walled carbon nanotube-modified glassy carbon electrode has been developed. The sensitive element, ie, tyrosinase, was immobilized using a drop-and-dry method followed by cross-linking. Tyrosinase maintained high bioactivity on this nanomaterial, catalyzing the oxidation of epinephrine to epinephrine-quinone, which was electrochemically reduced (-0.07 V versus Ag/AgCl) on the biosensor surface. Under optimum conditions, the biosensor showed a linear response in the range of 10–110 µM. The limit of detection was calculated to be 2.54 µM with a correlation coefficient of 0.977. The repeatability, expressed as the relative standard deviation for five consecutive determinations of 10-5 M epinephrine solution was 3.4%. A good correlation was obtained between results obtained by the biosensor and those obtained by ultraviolet spectrophotometric methods.Keywords: amperometry, single-walled carbon nanotubes, spectrophotometry, catecholamine, pharmaceutical formulaApetrei IMApetrei CDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2013, Iss Issue 1, Pp 4391-4398 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Apetrei IM Apetrei C Biosensor based on tyrosinase immobilized on a single-walled carbon nanotube-modified glassy carbon electrode for detection of epinephrine
description	Irina Mirela Apetrei,1 Constantin Apetrei21Department of Pharmaceutical Sciences, Faculty of Medicine and Pharmacy, 2Department of Chemistry, Physics and Environment, Faculty of Sciences and Environment, Dunarea de Jos University of Galati, RomaniaAbstract: A biosensor comprising tyrosinase immobilized on a single-walled carbon nanotube-modified glassy carbon electrode has been developed. The sensitive element, ie, tyrosinase, was immobilized using a drop-and-dry method followed by cross-linking. Tyrosinase maintained high bioactivity on this nanomaterial, catalyzing the oxidation of epinephrine to epinephrine-quinone, which was electrochemically reduced (-0.07 V versus Ag/AgCl) on the biosensor surface. Under optimum conditions, the biosensor showed a linear response in the range of 10–110 µM. The limit of detection was calculated to be 2.54 µM with a correlation coefficient of 0.977. The repeatability, expressed as the relative standard deviation for five consecutive determinations of 10-5 M epinephrine solution was 3.4%. A good correlation was obtained between results obtained by the biosensor and those obtained by ultraviolet spectrophotometric methods.Keywords: amperometry, single-walled carbon nanotubes, spectrophotometry, catecholamine, pharmaceutical formula
format	article
author	Apetrei IM Apetrei C
author_facet	Apetrei IM Apetrei C
author_sort	Apetrei IM
title	Biosensor based on tyrosinase immobilized on a single-walled carbon nanotube-modified glassy carbon electrode for detection of epinephrine
title_short	Biosensor based on tyrosinase immobilized on a single-walled carbon nanotube-modified glassy carbon electrode for detection of epinephrine
title_full	Biosensor based on tyrosinase immobilized on a single-walled carbon nanotube-modified glassy carbon electrode for detection of epinephrine
title_fullStr	Biosensor based on tyrosinase immobilized on a single-walled carbon nanotube-modified glassy carbon electrode for detection of epinephrine
title_full_unstemmed	Biosensor based on tyrosinase immobilized on a single-walled carbon nanotube-modified glassy carbon electrode for detection of epinephrine
title_sort	biosensor based on tyrosinase immobilized on a single-walled carbon nanotube-modified glassy carbon electrode for detection of epinephrine
publisher	Dove Medical Press
publishDate	2013
url	https://doaj.org/article/ce9f6b9ad6ee49209d1499c967cf1783
work_keys_str_mv	AT apetreiim biosensorbasedontyrosinaseimmobilizedonasinglewalledcarbonnanotubemodifiedglassycarbonelectrodefordetectionofepinephrine AT apetreic biosensorbasedontyrosinaseimmobilizedonasinglewalledcarbonnanotubemodifiedglassycarbonelectrodefordetectionofepinephrine
_version_	1718403225380454400

Biosensor based on tyrosinase immobilized on a single-walled carbon nanotube-modified glassy carbon electrode for detection of epinephrine

Ejemplares similares