Mesenchymal stem cells derived from human exfoliated deciduous teeth (SHEDs) induce immune modulatory profile in monocyte-derived dendritic cells.

<h4>Background</h4>Mesenchymal stem cells have prominent immune modulatory properties, which may have clinical applications; however their major source, bone marrow, is of limited availability. On the other hand, mesenchymal stem cells derived from human exfoliated deciduous teeth (SHEDs...

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Autores principales:	Fernando de Sá Silva, Rodrigo Nalio Ramos, Danilo Candido de Almeida, Enio Jose Bassi, Roberto Pereira Gonzales, Sueli Patricia Harumi Miyagi, Claudinéia Pereira Maranduba, Osvaldo Augusto Brazil Esteves Sant'Anna, Márcia Martins Marques, José Alexandre Marzagão Barbuto, Niels Olsen Saraiva Câmara, Carlos Magno da Costa Maranduba
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/ceb09f5d687241029f8c44df873a7489
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spelling	oai:doaj.org-article:ceb09f5d687241029f8c44df873a74892021-11-18T08:18:35ZMesenchymal stem cells derived from human exfoliated deciduous teeth (SHEDs) induce immune modulatory profile in monocyte-derived dendritic cells.1932-620310.1371/journal.pone.0098050https://doaj.org/article/ceb09f5d687241029f8c44df873a74892014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24846008/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>Mesenchymal stem cells have prominent immune modulatory properties, which may have clinical applications; however their major source, bone marrow, is of limited availability. On the other hand, mesenchymal stem cells derived from human exfoliated deciduous teeth (SHEDs) are readily accessible, but their immune regulatory properties have not been completely investigated. This study was designed, therefore, to evaluate the SHEDs influence on DCs differentiation, maturation, ability to activate T cells and to expand CD4(+)Foxp3(+) T cells.<h4>Methodology/principal findings</h4>The experiments were based in cellular co-culture during differentiation and maturation of monocyte derived-DCs (moDCs), with, or not, presence of SHEDs. After co-culture with SHEDs, (moDCs) presented lower expression of BDCA-1 and CD11c, in comparison to DC cultivated without SHEDs. CD40, CD80, CD83 and CD86 levels were also decreased in mature DCs (mDCs) after co-cultivation with SHEDs. To assess the ability of SHEDs-exposed moDCs to modulate T cell responses, the former were separated from SHEDs, and co-cultured with peripheral blood lymphocytes. After 5 days, the proliferation of CD4(+) and CD8(+) T cells was evaluated and found to be lower than that induced by moDCs cultivated without SHEDs. In addition, an increase in the proportion of CD4(+)Foxp3(+)IL-10(+) T cells was observed among cells stimulated by mature moDCs that were previously cultivated with SHEDs. Soluble factors released during co-cultures also showed a reduction in the pro-inflammatory cytokines (IL-2, TNF-α and IFN-γ), and an increase in the anti-inflammatory molecule IL-10.<h4>Conclusion/significance</h4>This study shows that SHEDs induce an immune regulatory phenotype in moDCs cells, evidenced by changes in maturation and differentiation rates, inhibition of lymphocyte stimulation and ability to expand CD4(+)Foxp3(+) T cells. Further characterization and validation of this phenomenon could support the use of SHEDs, directly or indirectly for immune modulation in the clinical practice.Fernando de Sá SilvaRodrigo Nalio RamosDanilo Candido de AlmeidaEnio Jose BassiRoberto Pereira GonzalesSueli Patricia Harumi MiyagiClaudinéia Pereira MarandubaOsvaldo Augusto Brazil Esteves Sant'AnnaMárcia Martins MarquesJosé Alexandre Marzagão BarbutoNiels Olsen Saraiva CâmaraCarlos Magno da Costa MarandubaPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 5, p e98050 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Fernando de Sá Silva Rodrigo Nalio Ramos Danilo Candido de Almeida Enio Jose Bassi Roberto Pereira Gonzales Sueli Patricia Harumi Miyagi Claudinéia Pereira Maranduba Osvaldo Augusto Brazil Esteves Sant'Anna Márcia Martins Marques José Alexandre Marzagão Barbuto Niels Olsen Saraiva Câmara Carlos Magno da Costa Maranduba Mesenchymal stem cells derived from human exfoliated deciduous teeth (SHEDs) induce immune modulatory profile in monocyte-derived dendritic cells.
description	<h4>Background</h4>Mesenchymal stem cells have prominent immune modulatory properties, which may have clinical applications; however their major source, bone marrow, is of limited availability. On the other hand, mesenchymal stem cells derived from human exfoliated deciduous teeth (SHEDs) are readily accessible, but their immune regulatory properties have not been completely investigated. This study was designed, therefore, to evaluate the SHEDs influence on DCs differentiation, maturation, ability to activate T cells and to expand CD4(+)Foxp3(+) T cells.<h4>Methodology/principal findings</h4>The experiments were based in cellular co-culture during differentiation and maturation of monocyte derived-DCs (moDCs), with, or not, presence of SHEDs. After co-culture with SHEDs, (moDCs) presented lower expression of BDCA-1 and CD11c, in comparison to DC cultivated without SHEDs. CD40, CD80, CD83 and CD86 levels were also decreased in mature DCs (mDCs) after co-cultivation with SHEDs. To assess the ability of SHEDs-exposed moDCs to modulate T cell responses, the former were separated from SHEDs, and co-cultured with peripheral blood lymphocytes. After 5 days, the proliferation of CD4(+) and CD8(+) T cells was evaluated and found to be lower than that induced by moDCs cultivated without SHEDs. In addition, an increase in the proportion of CD4(+)Foxp3(+)IL-10(+) T cells was observed among cells stimulated by mature moDCs that were previously cultivated with SHEDs. Soluble factors released during co-cultures also showed a reduction in the pro-inflammatory cytokines (IL-2, TNF-α and IFN-γ), and an increase in the anti-inflammatory molecule IL-10.<h4>Conclusion/significance</h4>This study shows that SHEDs induce an immune regulatory phenotype in moDCs cells, evidenced by changes in maturation and differentiation rates, inhibition of lymphocyte stimulation and ability to expand CD4(+)Foxp3(+) T cells. Further characterization and validation of this phenomenon could support the use of SHEDs, directly or indirectly for immune modulation in the clinical practice.
format	article
author	Fernando de Sá Silva Rodrigo Nalio Ramos Danilo Candido de Almeida Enio Jose Bassi Roberto Pereira Gonzales Sueli Patricia Harumi Miyagi Claudinéia Pereira Maranduba Osvaldo Augusto Brazil Esteves Sant'Anna Márcia Martins Marques José Alexandre Marzagão Barbuto Niels Olsen Saraiva Câmara Carlos Magno da Costa Maranduba
author_facet	Fernando de Sá Silva Rodrigo Nalio Ramos Danilo Candido de Almeida Enio Jose Bassi Roberto Pereira Gonzales Sueli Patricia Harumi Miyagi Claudinéia Pereira Maranduba Osvaldo Augusto Brazil Esteves Sant'Anna Márcia Martins Marques José Alexandre Marzagão Barbuto Niels Olsen Saraiva Câmara Carlos Magno da Costa Maranduba
author_sort	Fernando de Sá Silva
title	Mesenchymal stem cells derived from human exfoliated deciduous teeth (SHEDs) induce immune modulatory profile in monocyte-derived dendritic cells.
title_short	Mesenchymal stem cells derived from human exfoliated deciduous teeth (SHEDs) induce immune modulatory profile in monocyte-derived dendritic cells.
title_full	Mesenchymal stem cells derived from human exfoliated deciduous teeth (SHEDs) induce immune modulatory profile in monocyte-derived dendritic cells.
title_fullStr	Mesenchymal stem cells derived from human exfoliated deciduous teeth (SHEDs) induce immune modulatory profile in monocyte-derived dendritic cells.
title_full_unstemmed	Mesenchymal stem cells derived from human exfoliated deciduous teeth (SHEDs) induce immune modulatory profile in monocyte-derived dendritic cells.
title_sort	mesenchymal stem cells derived from human exfoliated deciduous teeth (sheds) induce immune modulatory profile in monocyte-derived dendritic cells.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/ceb09f5d687241029f8c44df873a7489
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Mesenchymal stem cells derived from human exfoliated deciduous teeth (SHEDs) induce immune modulatory profile in monocyte-derived dendritic cells.

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