Integration of chronological omics data reveals mitochondrial regulatory mechanisms during the development of hepatocellular carcinoma.

Mitochondria participate in multiple functions in eukaryotic cells. Although disruption of mitochondrial function has been associated with energetic deregulation in cancer, the chronological changes in mitochondria during cancer development remain unclear. With the aim to assess the role of mitochon...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	J Noé García-Chávez, Verónica R Vásquez-Garzón, Mercedes G López, Saúl Villa-Treviño, Rafael Montiel
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/ceb83fbd4f8c4f0d8f06a95ed5b310ea
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:ceb83fbd4f8c4f0d8f06a95ed5b310ea
record_format	dspace
spelling	oai:doaj.org-article:ceb83fbd4f8c4f0d8f06a95ed5b310ea2021-12-02T20:18:16ZIntegration of chronological omics data reveals mitochondrial regulatory mechanisms during the development of hepatocellular carcinoma.1932-620310.1371/journal.pone.0256016https://doaj.org/article/ceb83fbd4f8c4f0d8f06a95ed5b310ea2021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0256016https://doaj.org/toc/1932-6203Mitochondria participate in multiple functions in eukaryotic cells. Although disruption of mitochondrial function has been associated with energetic deregulation in cancer, the chronological changes in mitochondria during cancer development remain unclear. With the aim to assess the role of mitochondria throughout cancer development, we analyzed samples chronologically obtained from induced hepatocellular carcinoma (HCC) in rats. In our analyses, we integrated mitochondrial proteomic data, mitochondrial metabolomic data and nuclear genome transcriptomic data. We used pathway over-representation and weighted gene co-expression network analysis (WGCNA) to integrate expression profiles of genes, miRNAs, proteins and metabolite levels throughout HCC development. Our results show that mitochondria are dynamic organelles presenting specific modifications in different stages of HCC development. We also found that mitochondrial proteomic profiles from tissues adjacent to nodules or tumor are determined more by the stage of HCC development than by tissue type, and we evaluated two models to predict HCC stage of the samples using proteomic profiles. Finally, we propose an omics integration pipeline to massively identify molecular features that could be further evaluated as key regulators, biomarkers or therapeutic targets. As an example, we show a group of miRNAs and transcription factors as candidates, responsible for mitochondrial metabolic modification in HCC.J Noé García-ChávezVerónica R Vásquez-GarzónMercedes G LópezSaúl Villa-TreviñoRafael MontielPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 8, p e0256016 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q J Noé García-Chávez Verónica R Vásquez-Garzón Mercedes G López Saúl Villa-Treviño Rafael Montiel Integration of chronological omics data reveals mitochondrial regulatory mechanisms during the development of hepatocellular carcinoma.
description	Mitochondria participate in multiple functions in eukaryotic cells. Although disruption of mitochondrial function has been associated with energetic deregulation in cancer, the chronological changes in mitochondria during cancer development remain unclear. With the aim to assess the role of mitochondria throughout cancer development, we analyzed samples chronologically obtained from induced hepatocellular carcinoma (HCC) in rats. In our analyses, we integrated mitochondrial proteomic data, mitochondrial metabolomic data and nuclear genome transcriptomic data. We used pathway over-representation and weighted gene co-expression network analysis (WGCNA) to integrate expression profiles of genes, miRNAs, proteins and metabolite levels throughout HCC development. Our results show that mitochondria are dynamic organelles presenting specific modifications in different stages of HCC development. We also found that mitochondrial proteomic profiles from tissues adjacent to nodules or tumor are determined more by the stage of HCC development than by tissue type, and we evaluated two models to predict HCC stage of the samples using proteomic profiles. Finally, we propose an omics integration pipeline to massively identify molecular features that could be further evaluated as key regulators, biomarkers or therapeutic targets. As an example, we show a group of miRNAs and transcription factors as candidates, responsible for mitochondrial metabolic modification in HCC.
format	article
author	J Noé García-Chávez Verónica R Vásquez-Garzón Mercedes G López Saúl Villa-Treviño Rafael Montiel
author_facet	J Noé García-Chávez Verónica R Vásquez-Garzón Mercedes G López Saúl Villa-Treviño Rafael Montiel
author_sort	J Noé García-Chávez
title	Integration of chronological omics data reveals mitochondrial regulatory mechanisms during the development of hepatocellular carcinoma.
title_short	Integration of chronological omics data reveals mitochondrial regulatory mechanisms during the development of hepatocellular carcinoma.
title_full	Integration of chronological omics data reveals mitochondrial regulatory mechanisms during the development of hepatocellular carcinoma.
title_fullStr	Integration of chronological omics data reveals mitochondrial regulatory mechanisms during the development of hepatocellular carcinoma.
title_full_unstemmed	Integration of chronological omics data reveals mitochondrial regulatory mechanisms during the development of hepatocellular carcinoma.
title_sort	integration of chronological omics data reveals mitochondrial regulatory mechanisms during the development of hepatocellular carcinoma.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/ceb83fbd4f8c4f0d8f06a95ed5b310ea
work_keys_str_mv	AT jnoegarciachavez integrationofchronologicalomicsdatarevealsmitochondrialregulatorymechanismsduringthedevelopmentofhepatocellularcarcinoma AT veronicarvasquezgarzon integrationofchronologicalomicsdatarevealsmitochondrialregulatorymechanismsduringthedevelopmentofhepatocellularcarcinoma AT mercedesglopez integrationofchronologicalomicsdatarevealsmitochondrialregulatorymechanismsduringthedevelopmentofhepatocellularcarcinoma AT saulvillatrevino integrationofchronologicalomicsdatarevealsmitochondrialregulatorymechanismsduringthedevelopmentofhepatocellularcarcinoma AT rafaelmontiel integrationofchronologicalomicsdatarevealsmitochondrialregulatorymechanismsduringthedevelopmentofhepatocellularcarcinoma
_version_	1718374313658155008

Integration of chronological omics data reveals mitochondrial regulatory mechanisms during the development of hepatocellular carcinoma.

Ejemplares similares