Molecular detection of antibiotic resistance and virulence genes in staphylococcus species isolated from human and poultry
Staphylococcus species are the most precarious micro-organisms in poultry meat, because they cause multiple health problems, risks to consumers and the economy of the country. Since they act as antibiotic resistant bacteria that carry virulence genes and antibiotic resistance genes which can be t...
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| Autores principales: | , , |
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| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
South Valley University
2020
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| Materias: | |
| Acceso en línea: | https://doaj.org/article/ceb88b67cd18412181f049495a4f30ec |
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| Sumario: | Staphylococcus species are the most precarious micro-organisms in poultry meat, because they
cause multiple health problems, risks to consumers and the economy of the country. Since they
act as antibiotic resistant bacteria that carry virulence genes and antibiotic resistance genes which
can be transmitted from food producing animals as poultry to humans through various modes the
major one being food chain and cause health hazards for the consumers. Therefore, the current
study was aimed to investigate the incidence rate of Staphylococcus species isolated from broiler
chicken and hospitalized patient, detection the antimicrobial susceptibility of these isolates by
using Vitek2 system and confirmation the presence of genes encoding for pathogenicity and
antimicrobial resistance in detected isolates by PCR. To achieve these 200 samples were
collected from poultry farms and hospitalized patient (100 for each) in dissimilar districts in
Assiut province, to be subjected to bacteriological examination. The results showed that the
incidence of Staphylococcus species was 35% and 45% in poultry and human samples
respectively on mannitol agar. Vitek2 system divided poultry isolates to 11 isolates as coagulasepositive Staphylococci (CoPS) and 24 isolates as coagulase-negative staphylococci (CoNS).
Whereas 20 human isolates alienated as CoPS and 25 isolates assigned as CoNS. Antibiogram
cleared that 45.7% and 53.3% of Staphylococcus isolates from poultry and human were
identified as methicillin-resistant Staphylococci respectively, also Staphylococcal spp. cleared a
resistance to different types of antimicrobials such as penicillin, tetracycline clindamycin. Vitek2
system showed a significant ability to differentiate among Staphylococcus species and
distinguish its antimicrobial susceptibility which was complicated by conventional method. PCR
results showed that the Staphylococcal isolates of poultry and human were harboring genes
encoding for pathogenicity (coa, hld and pvl) and antimicrobial resistance (mecA, vanA, cfr and
BlaZ) while none of the isolates harbored sei and seh genes. So the obtained results emphasized
the importance of reducing the unwarranted use of antimicrobial agents and application of
sanitary hygienic measures in poultry production.
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