Chronic exposure of humans to high level natural background radiation leads to robust expression of protective stress response proteins

Abstract Understanding exposures to low doses of ionizing radiation are relevant since most environmental, diagnostic radiology and occupational exposures lie in this region. However, the molecular mechanisms that drive cellular responses at these doses, and the subsequent health outcomes, remain un...

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Autores principales:	S. Nishad, Pankaj Kumar Chauhan, R. Sowdhamini, Anu Ghosh
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/ceb949cc959840abb644561b8993022e
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spelling	oai:doaj.org-article:ceb949cc959840abb644561b8993022e2021-12-02T11:50:40ZChronic exposure of humans to high level natural background radiation leads to robust expression of protective stress response proteins10.1038/s41598-020-80405-y2045-2322https://doaj.org/article/ceb949cc959840abb644561b8993022e2021-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-80405-yhttps://doaj.org/toc/2045-2322Abstract Understanding exposures to low doses of ionizing radiation are relevant since most environmental, diagnostic radiology and occupational exposures lie in this region. However, the molecular mechanisms that drive cellular responses at these doses, and the subsequent health outcomes, remain unclear. A local monazite-rich high level natural radiation area (HLNRA) in the state of Kerala on the south-west coast of Indian subcontinent show radiation doses extending from ≤ 1 to ≥ 45 mGy/y and thus, serve as a model resource to understand low dose mechanisms directly on healthy humans. We performed quantitative discovery proteomics based on multiplexed isobaric tags (iTRAQ) coupled with LC–MS/MS on human peripheral blood mononuclear cells from HLNRA individuals. Several proteins involved in diverse biological processes such as DNA repair, RNA processing, chromatin modifications and cytoskeletal organization showed distinct expression in HLNRA individuals, suggestive of both recovery and adaptation to low dose radiation. In protein–protein interaction (PPI) networks, YWHAZ (14-3-3ζ) emerged as the top-most hub protein that may direct phosphorylation driven pro-survival cellular processes against radiation stress. PPI networks also identified an integral role for the cytoskeletal protein ACTB, signaling protein PRKACA; and the molecular chaperone HSPA8. The data will allow better integration of radiation biology and epidemiology for risk assessment [Data are available via ProteomeXchange with identifier PXD022380].S. NishadPankaj Kumar ChauhanR. SowdhaminiAnu GhoshNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-14 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q S. Nishad Pankaj Kumar Chauhan R. Sowdhamini Anu Ghosh Chronic exposure of humans to high level natural background radiation leads to robust expression of protective stress response proteins
description	Abstract Understanding exposures to low doses of ionizing radiation are relevant since most environmental, diagnostic radiology and occupational exposures lie in this region. However, the molecular mechanisms that drive cellular responses at these doses, and the subsequent health outcomes, remain unclear. A local monazite-rich high level natural radiation area (HLNRA) in the state of Kerala on the south-west coast of Indian subcontinent show radiation doses extending from ≤ 1 to ≥ 45 mGy/y and thus, serve as a model resource to understand low dose mechanisms directly on healthy humans. We performed quantitative discovery proteomics based on multiplexed isobaric tags (iTRAQ) coupled with LC–MS/MS on human peripheral blood mononuclear cells from HLNRA individuals. Several proteins involved in diverse biological processes such as DNA repair, RNA processing, chromatin modifications and cytoskeletal organization showed distinct expression in HLNRA individuals, suggestive of both recovery and adaptation to low dose radiation. In protein–protein interaction (PPI) networks, YWHAZ (14-3-3ζ) emerged as the top-most hub protein that may direct phosphorylation driven pro-survival cellular processes against radiation stress. PPI networks also identified an integral role for the cytoskeletal protein ACTB, signaling protein PRKACA; and the molecular chaperone HSPA8. The data will allow better integration of radiation biology and epidemiology for risk assessment [Data are available via ProteomeXchange with identifier PXD022380].
format	article
author	S. Nishad Pankaj Kumar Chauhan R. Sowdhamini Anu Ghosh
author_facet	S. Nishad Pankaj Kumar Chauhan R. Sowdhamini Anu Ghosh
author_sort	S. Nishad
title	Chronic exposure of humans to high level natural background radiation leads to robust expression of protective stress response proteins
title_short	Chronic exposure of humans to high level natural background radiation leads to robust expression of protective stress response proteins
title_full	Chronic exposure of humans to high level natural background radiation leads to robust expression of protective stress response proteins
title_fullStr	Chronic exposure of humans to high level natural background radiation leads to robust expression of protective stress response proteins
title_full_unstemmed	Chronic exposure of humans to high level natural background radiation leads to robust expression of protective stress response proteins
title_sort	chronic exposure of humans to high level natural background radiation leads to robust expression of protective stress response proteins
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/ceb949cc959840abb644561b8993022e
work_keys_str_mv	AT snishad chronicexposureofhumanstohighlevelnaturalbackgroundradiationleadstorobustexpressionofprotectivestressresponseproteins AT pankajkumarchauhan chronicexposureofhumanstohighlevelnaturalbackgroundradiationleadstorobustexpressionofprotectivestressresponseproteins AT rsowdhamini chronicexposureofhumanstohighlevelnaturalbackgroundradiationleadstorobustexpressionofprotectivestressresponseproteins AT anughosh chronicexposureofhumanstohighlevelnaturalbackgroundradiationleadstorobustexpressionofprotectivestressresponseproteins
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Chronic exposure of humans to high level natural background radiation leads to robust expression of protective stress response proteins

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