Characterization of P. vivax blood stage transcriptomes from field isolates reveals similarities among infections and complex gene isoforms

Abstract Our understanding of the structure and regulation of Plasmodium vivax genes is limited by our inability to grow the parasites in long-term in vitro cultures. Most P. vivax studies must therefore rely on patient samples, which typically display a low proportion of parasites and asynchronous...

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Autores principales:	Adam Kim, Jean Popovici, Amélie Vantaux, Reingsey Samreth, Sophalai Bin, Saorin Kim, Camille Roesch, Li Liang, Huw Davies, Philip Felgner, Sócrates Herrera, Myriam Arévalo-Herrera, Didier Ménard, David Serre
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/cec4e837a2a7481f9b1cc28fd298bd6f
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spelling	oai:doaj.org-article:cec4e837a2a7481f9b1cc28fd298bd6f2021-12-02T15:05:12ZCharacterization of P. vivax blood stage transcriptomes from field isolates reveals similarities among infections and complex gene isoforms10.1038/s41598-017-07275-92045-2322https://doaj.org/article/cec4e837a2a7481f9b1cc28fd298bd6f2017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-07275-9https://doaj.org/toc/2045-2322Abstract Our understanding of the structure and regulation of Plasmodium vivax genes is limited by our inability to grow the parasites in long-term in vitro cultures. Most P. vivax studies must therefore rely on patient samples, which typically display a low proportion of parasites and asynchronous parasites. Here, we present stranded RNA-seq data generated directly from a small volume of blood from three Cambodian vivax malaria patients collected before treatment. Our analyses show surprising similarities of the parasite gene expression patterns across infections, despite extensive variations in parasite stage proportion. These similarities contrast with the unique gene expression patterns observed in sporozoites isolated from salivary glands of infected Colombian mosquitoes. Our analyses also indicate that more than 10% of P. vivax genes encode multiple, often undescribed, protein-coding sequences, potentially increasing the diversity of proteins synthesized by blood stage parasites. These data also greatly improve the annotations of P. vivax gene untranslated regions, providing an important resource for future studies of specific genes.Adam KimJean PopoviciAmélie VantauxReingsey SamrethSophalai BinSaorin KimCamille RoeschLi LiangHuw DaviesPhilip FelgnerSócrates HerreraMyriam Arévalo-HerreraDidier MénardDavid SerreNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-12 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Adam Kim Jean Popovici Amélie Vantaux Reingsey Samreth Sophalai Bin Saorin Kim Camille Roesch Li Liang Huw Davies Philip Felgner Sócrates Herrera Myriam Arévalo-Herrera Didier Ménard David Serre Characterization of P. vivax blood stage transcriptomes from field isolates reveals similarities among infections and complex gene isoforms
description	Abstract Our understanding of the structure and regulation of Plasmodium vivax genes is limited by our inability to grow the parasites in long-term in vitro cultures. Most P. vivax studies must therefore rely on patient samples, which typically display a low proportion of parasites and asynchronous parasites. Here, we present stranded RNA-seq data generated directly from a small volume of blood from three Cambodian vivax malaria patients collected before treatment. Our analyses show surprising similarities of the parasite gene expression patterns across infections, despite extensive variations in parasite stage proportion. These similarities contrast with the unique gene expression patterns observed in sporozoites isolated from salivary glands of infected Colombian mosquitoes. Our analyses also indicate that more than 10% of P. vivax genes encode multiple, often undescribed, protein-coding sequences, potentially increasing the diversity of proteins synthesized by blood stage parasites. These data also greatly improve the annotations of P. vivax gene untranslated regions, providing an important resource for future studies of specific genes.
format	article
author	Adam Kim Jean Popovici Amélie Vantaux Reingsey Samreth Sophalai Bin Saorin Kim Camille Roesch Li Liang Huw Davies Philip Felgner Sócrates Herrera Myriam Arévalo-Herrera Didier Ménard David Serre
author_facet	Adam Kim Jean Popovici Amélie Vantaux Reingsey Samreth Sophalai Bin Saorin Kim Camille Roesch Li Liang Huw Davies Philip Felgner Sócrates Herrera Myriam Arévalo-Herrera Didier Ménard David Serre
author_sort	Adam Kim
title	Characterization of P. vivax blood stage transcriptomes from field isolates reveals similarities among infections and complex gene isoforms
title_short	Characterization of P. vivax blood stage transcriptomes from field isolates reveals similarities among infections and complex gene isoforms
title_full	Characterization of P. vivax blood stage transcriptomes from field isolates reveals similarities among infections and complex gene isoforms
title_fullStr	Characterization of P. vivax blood stage transcriptomes from field isolates reveals similarities among infections and complex gene isoforms
title_full_unstemmed	Characterization of P. vivax blood stage transcriptomes from field isolates reveals similarities among infections and complex gene isoforms
title_sort	characterization of p. vivax blood stage transcriptomes from field isolates reveals similarities among infections and complex gene isoforms
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/cec4e837a2a7481f9b1cc28fd298bd6f
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Characterization of P. vivax blood stage transcriptomes from field isolates reveals similarities among infections and complex gene isoforms

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