Regulation of Osteoclast Differentiation by Myosin X

Abstract Osteoclasts begin as mononuclear cells that fuse to form multinuclear cells able to resorb bone. The mechanisms that regulate all the steps of osteoclast differentiation are not entirely known. MYO10, an unconventional myosin, has previously been shown in mature osteoclasts to play a role i...

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Autores principales:	Amy Tasca, Kristina Astleford, Ari Lederman, Eric D. Jensen, Beth S. Lee, Rajaram Gopalakrishnan, Kim C. Mansky
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/cec4fadd19f14b0895c4665d05c0fb52
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spelling	oai:doaj.org-article:cec4fadd19f14b0895c4665d05c0fb522021-12-02T12:31:54ZRegulation of Osteoclast Differentiation by Myosin X10.1038/s41598-017-07855-92045-2322https://doaj.org/article/cec4fadd19f14b0895c4665d05c0fb522017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-07855-9https://doaj.org/toc/2045-2322Abstract Osteoclasts begin as mononuclear cells that fuse to form multinuclear cells able to resorb bone. The mechanisms that regulate all the steps of osteoclast differentiation are not entirely known. MYO10, an unconventional myosin, has previously been shown in mature osteoclasts to play a role in attachment and podosome positioning. We determined that MYO10 is also expressed early during osteoclast differentiation. Loss of MYO10 expression in osteoclast precursors inhibits the ability of mononuclear osteoclasts to fuse into multinuclear osteoclasts. Expression of Nfatc1, Dc-stamp, Ctsk, and β 3 integrin is reduced in the osteoclasts with reduced MYO10 expression. A slight reduction in the osteoclasts ability to migrate, as well as a reduction in SMAD 1/5/8 phosphorylation are also noted with reduced MYO10 expression. Interestingly we also detected a change in the ability of the osteoclast precursors to form tunneling nanotubes (TNTs), which suggests that MYO10 may regulate the presence of TNTs through its interaction with the cytoskeletal proteins.Amy TascaKristina AstlefordAri LedermanEric D. JensenBeth S. LeeRajaram GopalakrishnanKim C. ManskyNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-10 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Amy Tasca Kristina Astleford Ari Lederman Eric D. Jensen Beth S. Lee Rajaram Gopalakrishnan Kim C. Mansky Regulation of Osteoclast Differentiation by Myosin X
description	Abstract Osteoclasts begin as mononuclear cells that fuse to form multinuclear cells able to resorb bone. The mechanisms that regulate all the steps of osteoclast differentiation are not entirely known. MYO10, an unconventional myosin, has previously been shown in mature osteoclasts to play a role in attachment and podosome positioning. We determined that MYO10 is also expressed early during osteoclast differentiation. Loss of MYO10 expression in osteoclast precursors inhibits the ability of mononuclear osteoclasts to fuse into multinuclear osteoclasts. Expression of Nfatc1, Dc-stamp, Ctsk, and β 3 integrin is reduced in the osteoclasts with reduced MYO10 expression. A slight reduction in the osteoclasts ability to migrate, as well as a reduction in SMAD 1/5/8 phosphorylation are also noted with reduced MYO10 expression. Interestingly we also detected a change in the ability of the osteoclast precursors to form tunneling nanotubes (TNTs), which suggests that MYO10 may regulate the presence of TNTs through its interaction with the cytoskeletal proteins.
format	article
author	Amy Tasca Kristina Astleford Ari Lederman Eric D. Jensen Beth S. Lee Rajaram Gopalakrishnan Kim C. Mansky
author_facet	Amy Tasca Kristina Astleford Ari Lederman Eric D. Jensen Beth S. Lee Rajaram Gopalakrishnan Kim C. Mansky
author_sort	Amy Tasca
title	Regulation of Osteoclast Differentiation by Myosin X
title_short	Regulation of Osteoclast Differentiation by Myosin X
title_full	Regulation of Osteoclast Differentiation by Myosin X
title_fullStr	Regulation of Osteoclast Differentiation by Myosin X
title_full_unstemmed	Regulation of Osteoclast Differentiation by Myosin X
title_sort	regulation of osteoclast differentiation by myosin x
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/cec4fadd19f14b0895c4665d05c0fb52
work_keys_str_mv	AT amytasca regulationofosteoclastdifferentiationbymyosinx AT kristinaastleford regulationofosteoclastdifferentiationbymyosinx AT arilederman regulationofosteoclastdifferentiationbymyosinx AT ericdjensen regulationofosteoclastdifferentiationbymyosinx AT bethslee regulationofosteoclastdifferentiationbymyosinx AT rajaramgopalakrishnan regulationofosteoclastdifferentiationbymyosinx AT kimcmansky regulationofosteoclastdifferentiationbymyosinx
_version_	1718394244302897152

Regulation of Osteoclast Differentiation by Myosin X

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