Transcriptome analysis of "Candidatus Liberibacter solanacearum" in its psyllid vector, Bactericera cockerelli.

"Candidatus Liberibacter solanacearum" (Lso) is an emergent pathogen of carrots in Europe and solanaceous plants in North and Central America and New Zealand. This bacterium is closely related to other pathogenic Candidatus Liberibacter spp., all vectored by psyllids. In order to understan...

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Autores principales:	Freddy Ibanez, Julien Levy, Cecilia Tamborindeguy
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/cec5175d29a24e6b93f4d2240e5a4dde
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spelling	oai:doaj.org-article:cec5175d29a24e6b93f4d2240e5a4dde2021-11-25T06:09:41ZTranscriptome analysis of "Candidatus Liberibacter solanacearum" in its psyllid vector, Bactericera cockerelli.1932-620310.1371/journal.pone.0100955https://doaj.org/article/cec5175d29a24e6b93f4d2240e5a4dde2014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24992557/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203"Candidatus Liberibacter solanacearum" (Lso) is an emergent pathogen of carrots in Europe and solanaceous plants in North and Central America and New Zealand. This bacterium is closely related to other pathogenic Candidatus Liberibacter spp., all vectored by psyllids. In order to understand the molecular interaction of this pathogen and its psyllid vector, Bactericera cockerelli, Illumina sequencing of psyllid harboring Lso was performed to determine if this approach could be used to assess the bacterial transcriptome in this association. Prior to sequencing, psyllid RNA was purified and insect and bacterial rRNA were removed. Mapping of reads to Lso genome revealed that over 92% of the bacterial genes were expressed in the vector, and that the COG categories Translation and Post-translational modification, protein turnover, chaperone functions were the most expressed functional categories. Expression levels of selected Lso genes were confirmed by RT-qPCR. The transcriptomic analysis also helped correct Lso genome annotation by identifying the expression of genes that were not predicted in the genome sequencing effort.Freddy IbanezJulien LevyCecilia TamborindeguyPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 7, p e100955 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Freddy Ibanez Julien Levy Cecilia Tamborindeguy Transcriptome analysis of "Candidatus Liberibacter solanacearum" in its psyllid vector, Bactericera cockerelli.
description	"Candidatus Liberibacter solanacearum" (Lso) is an emergent pathogen of carrots in Europe and solanaceous plants in North and Central America and New Zealand. This bacterium is closely related to other pathogenic Candidatus Liberibacter spp., all vectored by psyllids. In order to understand the molecular interaction of this pathogen and its psyllid vector, Bactericera cockerelli, Illumina sequencing of psyllid harboring Lso was performed to determine if this approach could be used to assess the bacterial transcriptome in this association. Prior to sequencing, psyllid RNA was purified and insect and bacterial rRNA were removed. Mapping of reads to Lso genome revealed that over 92% of the bacterial genes were expressed in the vector, and that the COG categories Translation and Post-translational modification, protein turnover, chaperone functions were the most expressed functional categories. Expression levels of selected Lso genes were confirmed by RT-qPCR. The transcriptomic analysis also helped correct Lso genome annotation by identifying the expression of genes that were not predicted in the genome sequencing effort.
format	article
author	Freddy Ibanez Julien Levy Cecilia Tamborindeguy
author_facet	Freddy Ibanez Julien Levy Cecilia Tamborindeguy
author_sort	Freddy Ibanez
title	Transcriptome analysis of "Candidatus Liberibacter solanacearum" in its psyllid vector, Bactericera cockerelli.
title_short	Transcriptome analysis of "Candidatus Liberibacter solanacearum" in its psyllid vector, Bactericera cockerelli.
title_full	Transcriptome analysis of "Candidatus Liberibacter solanacearum" in its psyllid vector, Bactericera cockerelli.
title_fullStr	Transcriptome analysis of "Candidatus Liberibacter solanacearum" in its psyllid vector, Bactericera cockerelli.
title_full_unstemmed	Transcriptome analysis of "Candidatus Liberibacter solanacearum" in its psyllid vector, Bactericera cockerelli.
title_sort	transcriptome analysis of "candidatus liberibacter solanacearum" in its psyllid vector, bactericera cockerelli.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/cec5175d29a24e6b93f4d2240e5a4dde
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Transcriptome analysis of "Candidatus Liberibacter solanacearum" in its psyllid vector, Bactericera cockerelli.

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