A Biomimetic Heparinized Composite Silk-Based Vascular Scaffold with sustained Antithrombogenicity

Abstract Autologous grafts, as the gold standard for vascular bypass procedures, associated with several problems that limit their usability, so tissue engineered vessels have been the subject of an increasing number of works. Nevertheless, gathering all of the desired characteristics of vascular sc...

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Autores principales:	Masoud Zamani, Mona Khafaji, Mohammad Naji, Manouchehr Vossoughi, Iran Alemzadeh, Nooshin Haghighipour
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/ced2e15bb1a6466d99c60c1888ad6821
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spelling	oai:doaj.org-article:ced2e15bb1a6466d99c60c1888ad68212021-12-02T16:06:04ZA Biomimetic Heparinized Composite Silk-Based Vascular Scaffold with sustained Antithrombogenicity10.1038/s41598-017-04510-12045-2322https://doaj.org/article/ced2e15bb1a6466d99c60c1888ad68212017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-04510-1https://doaj.org/toc/2045-2322Abstract Autologous grafts, as the gold standard for vascular bypass procedures, associated with several problems that limit their usability, so tissue engineered vessels have been the subject of an increasing number of works. Nevertheless, gathering all of the desired characteristics of vascular scaffolds in the same construct has been a big challenge for scientists. Herein, a composite silk-based vascular scaffold (CSVS) was proposed to consider all the mechanical, structural and biological requirements of a small-diameter vascular scaffold. The scaffold’s lumen composed of braided silk fiber-reinforced silk fibroin (SF) sponge covalently heparinized (H-CSVS) using Hydroxy-Iron Complexes (HICs) as linkers. The highly porous SF external layer with pores above 60 μm was obtained by lyophilization. Silk fibers were fully embedded in scaffold’s wall with no delamination. The H-CSVS exhibited much higher burst pressure and suture retention strength than native vessels while comparable elastic modulus and compliance. H-CSVSs presented milder hemolysis in vitro and significant calcification resistance in subcutaneous implantation compared to non-heparinized ones. The in vitro antithrombogenic activity was sustained for over 12 weeks. The cytocompatibility was approved using endothelial cells (ECs) and vascular smooth muscle cells (SMCs) in vitro. Therefore, H-CSVS demonstrates a promising candidate for engineering of small-diameter vessels.Masoud ZamaniMona KhafajiMohammad NajiManouchehr VossoughiIran AlemzadehNooshin HaghighipourNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-14 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Masoud Zamani Mona Khafaji Mohammad Naji Manouchehr Vossoughi Iran Alemzadeh Nooshin Haghighipour A Biomimetic Heparinized Composite Silk-Based Vascular Scaffold with sustained Antithrombogenicity
description	Abstract Autologous grafts, as the gold standard for vascular bypass procedures, associated with several problems that limit their usability, so tissue engineered vessels have been the subject of an increasing number of works. Nevertheless, gathering all of the desired characteristics of vascular scaffolds in the same construct has been a big challenge for scientists. Herein, a composite silk-based vascular scaffold (CSVS) was proposed to consider all the mechanical, structural and biological requirements of a small-diameter vascular scaffold. The scaffold’s lumen composed of braided silk fiber-reinforced silk fibroin (SF) sponge covalently heparinized (H-CSVS) using Hydroxy-Iron Complexes (HICs) as linkers. The highly porous SF external layer with pores above 60 μm was obtained by lyophilization. Silk fibers were fully embedded in scaffold’s wall with no delamination. The H-CSVS exhibited much higher burst pressure and suture retention strength than native vessels while comparable elastic modulus and compliance. H-CSVSs presented milder hemolysis in vitro and significant calcification resistance in subcutaneous implantation compared to non-heparinized ones. The in vitro antithrombogenic activity was sustained for over 12 weeks. The cytocompatibility was approved using endothelial cells (ECs) and vascular smooth muscle cells (SMCs) in vitro. Therefore, H-CSVS demonstrates a promising candidate for engineering of small-diameter vessels.
format	article
author	Masoud Zamani Mona Khafaji Mohammad Naji Manouchehr Vossoughi Iran Alemzadeh Nooshin Haghighipour
author_facet	Masoud Zamani Mona Khafaji Mohammad Naji Manouchehr Vossoughi Iran Alemzadeh Nooshin Haghighipour
author_sort	Masoud Zamani
title	A Biomimetic Heparinized Composite Silk-Based Vascular Scaffold with sustained Antithrombogenicity
title_short	A Biomimetic Heparinized Composite Silk-Based Vascular Scaffold with sustained Antithrombogenicity
title_full	A Biomimetic Heparinized Composite Silk-Based Vascular Scaffold with sustained Antithrombogenicity
title_fullStr	A Biomimetic Heparinized Composite Silk-Based Vascular Scaffold with sustained Antithrombogenicity
title_full_unstemmed	A Biomimetic Heparinized Composite Silk-Based Vascular Scaffold with sustained Antithrombogenicity
title_sort	biomimetic heparinized composite silk-based vascular scaffold with sustained antithrombogenicity
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/ced2e15bb1a6466d99c60c1888ad6821
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A Biomimetic Heparinized Composite Silk-Based Vascular Scaffold with sustained Antithrombogenicity

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