Consideration of Epstein-Barr Virus-Encoded Noncoding RNAs EBER1 and EBER2 as a Functional Backup of Viral Oncoprotein Latent Membrane Protein 1

ABSTRACT The Epstein-Barr virus (EBV)-encoded noncoding RNAs EBER1 and EBER2 are highly abundant through all four latency stages of EBV infection (III-II-I-0) and have been associated with an oncogenic phenotype when expressed in cell lines cultured in vitro. In vivo, EBV-infected B cells derived fr...

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Autores principales: Kristina M. Herbert, Genaro Pimienta
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Lenguaje:EN
Publicado: American Society for Microbiology 2016
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Acceso en línea:https://doaj.org/article/ced2e1d4670b4632b5df2403acefa80d
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spelling oai:doaj.org-article:ced2e1d4670b4632b5df2403acefa80d2021-11-15T15:49:40ZConsideration of Epstein-Barr Virus-Encoded Noncoding RNAs EBER1 and EBER2 as a Functional Backup of Viral Oncoprotein Latent Membrane Protein 110.1128/mBio.01926-152150-7511https://doaj.org/article/ced2e1d4670b4632b5df2403acefa80d2016-03-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mBio.01926-15https://doaj.org/toc/2150-7511ABSTRACT The Epstein-Barr virus (EBV)-encoded noncoding RNAs EBER1 and EBER2 are highly abundant through all four latency stages of EBV infection (III-II-I-0) and have been associated with an oncogenic phenotype when expressed in cell lines cultured in vitro. In vivo, EBV-infected B cells derived from freshly isolated lymphocytes show that EBER1/2 deletion does not impair viral latency. Based on published quantitative proteomics data from BJAB cells expressing EBER1 and EBER2, we propose that the EBERs, through their activation of AKT in a B-cell-specific manner, are a functionally redundant backup of latent membrane protein 1 (LMP1)—an essential oncoprotein in EBV-associated malignancies, with a main role in AKT activation. Our proposed model may explain the lack of effect on viral latency establishment in EBER-minus EBV infection.Kristina M. HerbertGenaro PimientaAmerican Society for MicrobiologyarticleMicrobiologyQR1-502ENmBio, Vol 7, Iss 1 (2016)
institution DOAJ
collection DOAJ
language EN
topic Microbiology
QR1-502
spellingShingle Microbiology
QR1-502
Kristina M. Herbert
Genaro Pimienta
Consideration of Epstein-Barr Virus-Encoded Noncoding RNAs EBER1 and EBER2 as a Functional Backup of Viral Oncoprotein Latent Membrane Protein 1
description ABSTRACT The Epstein-Barr virus (EBV)-encoded noncoding RNAs EBER1 and EBER2 are highly abundant through all four latency stages of EBV infection (III-II-I-0) and have been associated with an oncogenic phenotype when expressed in cell lines cultured in vitro. In vivo, EBV-infected B cells derived from freshly isolated lymphocytes show that EBER1/2 deletion does not impair viral latency. Based on published quantitative proteomics data from BJAB cells expressing EBER1 and EBER2, we propose that the EBERs, through their activation of AKT in a B-cell-specific manner, are a functionally redundant backup of latent membrane protein 1 (LMP1)—an essential oncoprotein in EBV-associated malignancies, with a main role in AKT activation. Our proposed model may explain the lack of effect on viral latency establishment in EBER-minus EBV infection.
format article
author Kristina M. Herbert
Genaro Pimienta
author_facet Kristina M. Herbert
Genaro Pimienta
author_sort Kristina M. Herbert
title Consideration of Epstein-Barr Virus-Encoded Noncoding RNAs EBER1 and EBER2 as a Functional Backup of Viral Oncoprotein Latent Membrane Protein 1
title_short Consideration of Epstein-Barr Virus-Encoded Noncoding RNAs EBER1 and EBER2 as a Functional Backup of Viral Oncoprotein Latent Membrane Protein 1
title_full Consideration of Epstein-Barr Virus-Encoded Noncoding RNAs EBER1 and EBER2 as a Functional Backup of Viral Oncoprotein Latent Membrane Protein 1
title_fullStr Consideration of Epstein-Barr Virus-Encoded Noncoding RNAs EBER1 and EBER2 as a Functional Backup of Viral Oncoprotein Latent Membrane Protein 1
title_full_unstemmed Consideration of Epstein-Barr Virus-Encoded Noncoding RNAs EBER1 and EBER2 as a Functional Backup of Viral Oncoprotein Latent Membrane Protein 1
title_sort consideration of epstein-barr virus-encoded noncoding rnas eber1 and eber2 as a functional backup of viral oncoprotein latent membrane protein 1
publisher American Society for Microbiology
publishDate 2016
url https://doaj.org/article/ced2e1d4670b4632b5df2403acefa80d
work_keys_str_mv AT kristinamherbert considerationofepsteinbarrvirusencodednoncodingrnaseber1andeber2asafunctionalbackupofviraloncoproteinlatentmembraneprotein1
AT genaropimienta considerationofepsteinbarrvirusencodednoncodingrnaseber1andeber2asafunctionalbackupofviraloncoproteinlatentmembraneprotein1
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