SERS Investigation of Cancer Cells Treated with PDT: Quantification of Cell Survival and Follow-up

Abstract In this study Surface Enhanced Raman Spectroscopy (SERS) data recorded from mouse mammary glands cancer cells (4T1 cell line) was used to assess information regarding differences between control, death and viable cells after Photodynamic Therapy (PDT) treatment. The treatment used nanoemuls...

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Autores principales:	A. B. Veloso, J. P. F. Longo, L. A. Muehlmann, B. F. Tollstadius, P. E. N. Souza, R. B. Azevedo, P. C. Morais, S. W. da Silva
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/ced9a39f6acf470f9bc33258ba08b02a
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spelling	oai:doaj.org-article:ced9a39f6acf470f9bc33258ba08b02a2021-12-02T16:08:19ZSERS Investigation of Cancer Cells Treated with PDT: Quantification of Cell Survival and Follow-up10.1038/s41598-017-07469-12045-2322https://doaj.org/article/ced9a39f6acf470f9bc33258ba08b02a2017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-07469-1https://doaj.org/toc/2045-2322Abstract In this study Surface Enhanced Raman Spectroscopy (SERS) data recorded from mouse mammary glands cancer cells (4T1 cell line) was used to assess information regarding differences between control, death and viable cells after Photodynamic Therapy (PDT) treatment. The treatment used nanoemulsions (NE/PS) loaded with different chloroaluminumphthalocyanine (ClAlP) photosensitizer (PS) contents (5 and 10 µmol × L−1) and illumination (660 nm wavelength) at 10 J × cm−2 (10 minutes). The SERS data revealed significant molecular alterations in proteins and lipids due to the PDT treatment. Principal Component Analysis (PCA) was applied to analyze the data recorded. Three-dimensional and well reproductive PCA scatter plots were obtained, revealing that two clusters of dead cells were well separated from one another and from control cluster. Overlap between two clusters of viable cells was observed, though well separated from control cluster. Moreover, the data analysis also pointed out necrosis as the main cell death mechanism induced by the PDT, in agreement with the literature. Finally, Raman modes peaking at 608 cm−1 (proteins) and 1231 cm−1 (lipids) can be selected for follow up of survival rate of neoplastic cells after PDT. We envisage that this finding is key to contribute to a quick development of quantitative infrared thermography imaging.A. B. VelosoJ. P. F. LongoL. A. MuehlmannB. F. TollstadiusP. E. N. SouzaR. B. AzevedoP. C. MoraisS. W. da SilvaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-12 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q A. B. Veloso J. P. F. Longo L. A. Muehlmann B. F. Tollstadius P. E. N. Souza R. B. Azevedo P. C. Morais S. W. da Silva SERS Investigation of Cancer Cells Treated with PDT: Quantification of Cell Survival and Follow-up
description	Abstract In this study Surface Enhanced Raman Spectroscopy (SERS) data recorded from mouse mammary glands cancer cells (4T1 cell line) was used to assess information regarding differences between control, death and viable cells after Photodynamic Therapy (PDT) treatment. The treatment used nanoemulsions (NE/PS) loaded with different chloroaluminumphthalocyanine (ClAlP) photosensitizer (PS) contents (5 and 10 µmol × L−1) and illumination (660 nm wavelength) at 10 J × cm−2 (10 minutes). The SERS data revealed significant molecular alterations in proteins and lipids due to the PDT treatment. Principal Component Analysis (PCA) was applied to analyze the data recorded. Three-dimensional and well reproductive PCA scatter plots were obtained, revealing that two clusters of dead cells were well separated from one another and from control cluster. Overlap between two clusters of viable cells was observed, though well separated from control cluster. Moreover, the data analysis also pointed out necrosis as the main cell death mechanism induced by the PDT, in agreement with the literature. Finally, Raman modes peaking at 608 cm−1 (proteins) and 1231 cm−1 (lipids) can be selected for follow up of survival rate of neoplastic cells after PDT. We envisage that this finding is key to contribute to a quick development of quantitative infrared thermography imaging.
format	article
author	A. B. Veloso J. P. F. Longo L. A. Muehlmann B. F. Tollstadius P. E. N. Souza R. B. Azevedo P. C. Morais S. W. da Silva
author_facet	A. B. Veloso J. P. F. Longo L. A. Muehlmann B. F. Tollstadius P. E. N. Souza R. B. Azevedo P. C. Morais S. W. da Silva
author_sort	A. B. Veloso
title	SERS Investigation of Cancer Cells Treated with PDT: Quantification of Cell Survival and Follow-up
title_short	SERS Investigation of Cancer Cells Treated with PDT: Quantification of Cell Survival and Follow-up
title_full	SERS Investigation of Cancer Cells Treated with PDT: Quantification of Cell Survival and Follow-up
title_fullStr	SERS Investigation of Cancer Cells Treated with PDT: Quantification of Cell Survival and Follow-up
title_full_unstemmed	SERS Investigation of Cancer Cells Treated with PDT: Quantification of Cell Survival and Follow-up
title_sort	sers investigation of cancer cells treated with pdt: quantification of cell survival and follow-up
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/ced9a39f6acf470f9bc33258ba08b02a
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SERS Investigation of Cancer Cells Treated with PDT: Quantification of Cell Survival and Follow-up

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