Quadriwave lateral shearing interferometric microscopy with wideband sensitivity enhancement for quantitative phase imaging in real time

Abstract Real-time quantitative phase imaging has tremendous potential in investigating live biological specimens in vitro. Here we report on a wideband sensitivity-enhanced interferometric microscopy for quantitative phase imaging in real time by employing two quadriwave lateral shearing interferom...

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Autores principales:	Tong Ling, Jiabin Jiang, Rui Zhang, Yongying Yang
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/cedbfa53cc6541408b68a95f97c20857
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spelling	oai:doaj.org-article:cedbfa53cc6541408b68a95f97c208572021-12-02T11:51:12ZQuadriwave lateral shearing interferometric microscopy with wideband sensitivity enhancement for quantitative phase imaging in real time10.1038/s41598-017-00053-72045-2322https://doaj.org/article/cedbfa53cc6541408b68a95f97c208572017-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-00053-7https://doaj.org/toc/2045-2322Abstract Real-time quantitative phase imaging has tremendous potential in investigating live biological specimens in vitro. Here we report on a wideband sensitivity-enhanced interferometric microscopy for quantitative phase imaging in real time by employing two quadriwave lateral shearing interferometers based on randomly encoded hybrid gratings with different lateral shears. Theoretical framework to analyze the measurement sensitivity is firstly proposed, from which the optimal lateral shear pair for sensitivity enhancement is also derived. To accelerate the phase retrieval algorithm for real-time visualization, we develop a fully vectorized path-independent differential leveling phase unwrapping algorithm ready for parallel computing, and the framerate for retrieving the phase from each pair of two 4 mega pixel interferograms is able to reach 47.85 frames per second. Experiment results demonstrate that the wideband sensitivity-enhanced interferometric microscopy is capable of eliminating all the periodical error caused by spectral leaking problem and reducing the temporal standard deviation to the half level compared with phase directly retrieved by the interferogram. Due to its high adaptability, the wideband sensitivity-enhanced interferometric microscopy is promising in retrofitting existing microscopes to quantitative phase microscopes with high measurement precision and real-time visualization.Tong LingJiabin JiangRui ZhangYongying YangNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-14 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Tong Ling Jiabin Jiang Rui Zhang Yongying Yang Quadriwave lateral shearing interferometric microscopy with wideband sensitivity enhancement for quantitative phase imaging in real time
description	Abstract Real-time quantitative phase imaging has tremendous potential in investigating live biological specimens in vitro. Here we report on a wideband sensitivity-enhanced interferometric microscopy for quantitative phase imaging in real time by employing two quadriwave lateral shearing interferometers based on randomly encoded hybrid gratings with different lateral shears. Theoretical framework to analyze the measurement sensitivity is firstly proposed, from which the optimal lateral shear pair for sensitivity enhancement is also derived. To accelerate the phase retrieval algorithm for real-time visualization, we develop a fully vectorized path-independent differential leveling phase unwrapping algorithm ready for parallel computing, and the framerate for retrieving the phase from each pair of two 4 mega pixel interferograms is able to reach 47.85 frames per second. Experiment results demonstrate that the wideband sensitivity-enhanced interferometric microscopy is capable of eliminating all the periodical error caused by spectral leaking problem and reducing the temporal standard deviation to the half level compared with phase directly retrieved by the interferogram. Due to its high adaptability, the wideband sensitivity-enhanced interferometric microscopy is promising in retrofitting existing microscopes to quantitative phase microscopes with high measurement precision and real-time visualization.
format	article
author	Tong Ling Jiabin Jiang Rui Zhang Yongying Yang
author_facet	Tong Ling Jiabin Jiang Rui Zhang Yongying Yang
author_sort	Tong Ling
title	Quadriwave lateral shearing interferometric microscopy with wideband sensitivity enhancement for quantitative phase imaging in real time
title_short	Quadriwave lateral shearing interferometric microscopy with wideband sensitivity enhancement for quantitative phase imaging in real time
title_full	Quadriwave lateral shearing interferometric microscopy with wideband sensitivity enhancement for quantitative phase imaging in real time
title_fullStr	Quadriwave lateral shearing interferometric microscopy with wideband sensitivity enhancement for quantitative phase imaging in real time
title_full_unstemmed	Quadriwave lateral shearing interferometric microscopy with wideband sensitivity enhancement for quantitative phase imaging in real time
title_sort	quadriwave lateral shearing interferometric microscopy with wideband sensitivity enhancement for quantitative phase imaging in real time
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/cedbfa53cc6541408b68a95f97c20857
work_keys_str_mv	AT tongling quadriwavelateralshearinginterferometricmicroscopywithwidebandsensitivityenhancementforquantitativephaseimaginginrealtime AT jiabinjiang quadriwavelateralshearinginterferometricmicroscopywithwidebandsensitivityenhancementforquantitativephaseimaginginrealtime AT ruizhang quadriwavelateralshearinginterferometricmicroscopywithwidebandsensitivityenhancementforquantitativephaseimaginginrealtime AT yongyingyang quadriwavelateralshearinginterferometricmicroscopywithwidebandsensitivityenhancementforquantitativephaseimaginginrealtime
_version_	1718395191682924544

Quadriwave lateral shearing interferometric microscopy with wideband sensitivity enhancement for quantitative phase imaging in real time

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