Thidiazuron-enhanced biosynthesis and antimicrobial efficacy of silver nanoparticles via improving phytochemical reducing potential in callus culture of Linum usitatissimum L.

Sumaira Anjum, Bilal Haider AbbasiDepartment of Biotechnology, Quaid-i-Azam University, Islamabad, PakistanAbstract: Green synthesis of silver nanoparticles (AgNPs) by using plants is an emerging class of nanobiotechnology. It revolutionizes all domains of medical sciences by synthesizing chemical-f...

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Autores principales:	Anjum S, Abbasi BH
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2016
Materias:	Silver nanoparticles Linum usitatissimum L. In vitro cultures Thidiazuron Antimicrobial Multidrug resistant bacteria Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/cedd9c4befd74fe2b6afc273562beca4
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spelling	oai:doaj.org-article:cedd9c4befd74fe2b6afc273562beca42021-12-02T03:10:57ZThidiazuron-enhanced biosynthesis and antimicrobial efficacy of silver nanoparticles via improving phytochemical reducing potential in callus culture of Linum usitatissimum L.1178-2013https://doaj.org/article/cedd9c4befd74fe2b6afc273562beca42016-02-01T00:00:00Zhttps://www.dovepress.com/thidiazuron-enhanced-biosynthesis-and-antimicrobial-efficacy-of-silver-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Sumaira Anjum, Bilal Haider AbbasiDepartment of Biotechnology, Quaid-i-Azam University, Islamabad, PakistanAbstract: Green synthesis of silver nanoparticles (AgNPs) by using plants is an emerging class of nanobiotechnology. It revolutionizes all domains of medical sciences by synthesizing chemical-free AgNPs for various biomedical applications. In this report, AgNPs were successfully synthesized by using whole plant extract (WPE) and thidiazuron-induced callus extract (CE) of Linum usitatissimum. The phytochemical analysis revealed that the total phenolic and flavonoid contents were higher in CE than that in WPE. Ultraviolet-visible spectroscopy of synthesized AgNPs showed a characteristic surface plasmon band in the range of 410–426 nm. Bioreduction of CE-mediated AgNPs was completed in a shorter time than that of WPE-mediated AgNPs. Scanning electron microscopy showed that both types of synthesized AgNPs were spherical in shape, but CE-mediated AgNPs were smaller in size (19–24 nm) and more scattered in distribution than that of WPE-mediated AgNPs (49–54 nm). X-ray diffraction analysis confirmed crystalline nature (face-centered cubic) of both types of AgNPs. Fourier-transform infrared spectroscopy revealed that the polyphenols and flavonoids were mainly responsible for reduction and capping of synthesized AgNPs. Energy dispersive X-ray analysis further confirmed the successful synthesis of AgNPs. Moreover, the synthesized AgNPs were found to be stable over months with no change in the surface plasmon bands. More importantly, CE-mediated AgNPs displayed significantly higher bactericidal activity against multiple drug-resistant human pathogens than WPE-mediated AgNPs. The present work highlighted the potent role of thidiazuron in in vitro-derived cultures for enhanced biosynthesis of chemical-free AgNPs, which can be used as nanomedicines in many biomedical applications.Keywords: silver nanoparticles, Linum usitatissimum L., in vitro cultures, thidiazuron, antimicrobial, multidrug-resistant bacteriaAnjum SAbbasi BHDove Medical PressarticleSilver nanoparticlesLinum usitatissimum L.In vitro culturesThidiazuronAntimicrobialMultidrug resistant bacteriaMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2016, Iss Issue 1, Pp 715-728 (2016)
institution	DOAJ
collection	DOAJ
language	EN
topic	Silver nanoparticles Linum usitatissimum L. In vitro cultures Thidiazuron Antimicrobial Multidrug resistant bacteria Medicine (General) R5-920
spellingShingle	Silver nanoparticles Linum usitatissimum L. In vitro cultures Thidiazuron Antimicrobial Multidrug resistant bacteria Medicine (General) R5-920 Anjum S Abbasi BH Thidiazuron-enhanced biosynthesis and antimicrobial efficacy of silver nanoparticles via improving phytochemical reducing potential in callus culture of Linum usitatissimum L.
description	Sumaira Anjum, Bilal Haider AbbasiDepartment of Biotechnology, Quaid-i-Azam University, Islamabad, PakistanAbstract: Green synthesis of silver nanoparticles (AgNPs) by using plants is an emerging class of nanobiotechnology. It revolutionizes all domains of medical sciences by synthesizing chemical-free AgNPs for various biomedical applications. In this report, AgNPs were successfully synthesized by using whole plant extract (WPE) and thidiazuron-induced callus extract (CE) of Linum usitatissimum. The phytochemical analysis revealed that the total phenolic and flavonoid contents were higher in CE than that in WPE. Ultraviolet-visible spectroscopy of synthesized AgNPs showed a characteristic surface plasmon band in the range of 410–426 nm. Bioreduction of CE-mediated AgNPs was completed in a shorter time than that of WPE-mediated AgNPs. Scanning electron microscopy showed that both types of synthesized AgNPs were spherical in shape, but CE-mediated AgNPs were smaller in size (19–24 nm) and more scattered in distribution than that of WPE-mediated AgNPs (49–54 nm). X-ray diffraction analysis confirmed crystalline nature (face-centered cubic) of both types of AgNPs. Fourier-transform infrared spectroscopy revealed that the polyphenols and flavonoids were mainly responsible for reduction and capping of synthesized AgNPs. Energy dispersive X-ray analysis further confirmed the successful synthesis of AgNPs. Moreover, the synthesized AgNPs were found to be stable over months with no change in the surface plasmon bands. More importantly, CE-mediated AgNPs displayed significantly higher bactericidal activity against multiple drug-resistant human pathogens than WPE-mediated AgNPs. The present work highlighted the potent role of thidiazuron in in vitro-derived cultures for enhanced biosynthesis of chemical-free AgNPs, which can be used as nanomedicines in many biomedical applications.Keywords: silver nanoparticles, Linum usitatissimum L., in vitro cultures, thidiazuron, antimicrobial, multidrug-resistant bacteria
format	article
author	Anjum S Abbasi BH
author_facet	Anjum S Abbasi BH
author_sort	Anjum S
title	Thidiazuron-enhanced biosynthesis and antimicrobial efficacy of silver nanoparticles via improving phytochemical reducing potential in callus culture of Linum usitatissimum L.
title_short	Thidiazuron-enhanced biosynthesis and antimicrobial efficacy of silver nanoparticles via improving phytochemical reducing potential in callus culture of Linum usitatissimum L.
title_full	Thidiazuron-enhanced biosynthesis and antimicrobial efficacy of silver nanoparticles via improving phytochemical reducing potential in callus culture of Linum usitatissimum L.
title_fullStr	Thidiazuron-enhanced biosynthesis and antimicrobial efficacy of silver nanoparticles via improving phytochemical reducing potential in callus culture of Linum usitatissimum L.
title_full_unstemmed	Thidiazuron-enhanced biosynthesis and antimicrobial efficacy of silver nanoparticles via improving phytochemical reducing potential in callus culture of Linum usitatissimum L.
title_sort	thidiazuron-enhanced biosynthesis and antimicrobial efficacy of silver nanoparticles via improving phytochemical reducing potential in callus culture of linum usitatissimum l.
publisher	Dove Medical Press
publishDate	2016
url	https://doaj.org/article/cedd9c4befd74fe2b6afc273562beca4
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Thidiazuron-enhanced biosynthesis and antimicrobial efficacy of silver nanoparticles via improving phytochemical reducing potential in callus culture of Linum usitatissimum L.

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