Reciprocation of micro-objects by contraction and extension of Vorticella convallaria using polylysine as adhesive material

Integration of bioactuators in engineered microstructures is expected to be beneficial to further miniaturize and functionalize microelectromechanical systems. However, it is difficult to achieve reciprocation of micro-objects with common biological motors, although reciprocating movement is an impo...

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Autores principales:	Moeto NAGAI, Hiroshi ASAI, Hiroyuki FUJITA
Formato:	article
Lenguaje:	EN
Publicado:	The Japan Society of Mechanical Engineers 2014
Materias:	biohybrid system reciprocating motion bioactuator vorticella micromechanical system Mechanical engineering and machinery TJ1-1570
Acceso en línea:	https://doaj.org/article/cee3b18672ac4317bf657126f9f53844
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spelling	oai:doaj.org-article:cee3b18672ac4317bf657126f9f538442021-11-26T06:09:52ZReciprocation of micro-objects by contraction and extension of Vorticella convallaria using polylysine as adhesive material2187-974510.1299/mej.2014mn0038https://doaj.org/article/cee3b18672ac4317bf657126f9f538442014-08-01T00:00:00Zhttps://www.jstage.jst.go.jp/article/mej/1/4/1_2014mn0038/_pdf/-char/enhttps://doaj.org/toc/2187-9745Integration of bioactuators in engineered microstructures is expected to be beneficial to further miniaturize and functionalize microelectromechanical systems. However, it is difficult to achieve reciprocation of micro-objects with common biological motors, although reciprocating movement is an important mechanism in constructing micromechanical systems. The ciliate protozoan Vorticella convallaria possesses a contractile filamentous stalk approximately 100 μm long, of which the contraction-extension cycle has the potential of being used as a linear reciprocating machine. In this study, we used polylysine (PLL) to attach micro-objects to Vorticella convallaria with the purpose of reciprocating the objects by contracting and extending the Vorticella cells. Two types of micro-objects, namely, polystyrene microspheres and glass grits, were coated with positively charged PLL and attached by electrostatic interaction to negatively charged V. convallaria. We characterized the adhesive performance and analyzed the movement of the objects by optical microscope observation. Microspheres of diameter 21 μm were moved back and forth by the contraction and extension of V. convallaria. Comparison of the adhesiveness of PLL-coated and COOH-terminated spheres confirmed the effectiveness of our attachment and actuation method. The grits were actuated in various ways depending on their size, the point of attachment to V. convallaria, and the number of cells. The typical motions were linear and rotational and were propelled by a few cells. V. convallaria cells displaced thin glass grits of thickness up to tens of micrometers. The adhesive force and the drag forces were estimated hydrodynamically. The implemented reciprocating motion can be applied to biohybrid microfluidic systems.Moeto NAGAIHiroshi ASAIHiroyuki FUJITAThe Japan Society of Mechanical Engineersarticlebiohybrid systemreciprocating motionbioactuatorvorticellamicromechanical systemMechanical engineering and machineryTJ1-1570ENMechanical Engineering Journal, Vol 1, Iss 4, Pp MN0038-MN0038 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	biohybrid system reciprocating motion bioactuator vorticella micromechanical system Mechanical engineering and machinery TJ1-1570
spellingShingle	biohybrid system reciprocating motion bioactuator vorticella micromechanical system Mechanical engineering and machinery TJ1-1570 Moeto NAGAI Hiroshi ASAI Hiroyuki FUJITA Reciprocation of micro-objects by contraction and extension of Vorticella convallaria using polylysine as adhesive material
description	Integration of bioactuators in engineered microstructures is expected to be beneficial to further miniaturize and functionalize microelectromechanical systems. However, it is difficult to achieve reciprocation of micro-objects with common biological motors, although reciprocating movement is an important mechanism in constructing micromechanical systems. The ciliate protozoan Vorticella convallaria possesses a contractile filamentous stalk approximately 100 μm long, of which the contraction-extension cycle has the potential of being used as a linear reciprocating machine. In this study, we used polylysine (PLL) to attach micro-objects to Vorticella convallaria with the purpose of reciprocating the objects by contracting and extending the Vorticella cells. Two types of micro-objects, namely, polystyrene microspheres and glass grits, were coated with positively charged PLL and attached by electrostatic interaction to negatively charged V. convallaria. We characterized the adhesive performance and analyzed the movement of the objects by optical microscope observation. Microspheres of diameter 21 μm were moved back and forth by the contraction and extension of V. convallaria. Comparison of the adhesiveness of PLL-coated and COOH-terminated spheres confirmed the effectiveness of our attachment and actuation method. The grits were actuated in various ways depending on their size, the point of attachment to V. convallaria, and the number of cells. The typical motions were linear and rotational and were propelled by a few cells. V. convallaria cells displaced thin glass grits of thickness up to tens of micrometers. The adhesive force and the drag forces were estimated hydrodynamically. The implemented reciprocating motion can be applied to biohybrid microfluidic systems.
format	article
author	Moeto NAGAI Hiroshi ASAI Hiroyuki FUJITA
author_facet	Moeto NAGAI Hiroshi ASAI Hiroyuki FUJITA
author_sort	Moeto NAGAI
title	Reciprocation of micro-objects by contraction and extension of Vorticella convallaria using polylysine as adhesive material
title_short	Reciprocation of micro-objects by contraction and extension of Vorticella convallaria using polylysine as adhesive material
title_full	Reciprocation of micro-objects by contraction and extension of Vorticella convallaria using polylysine as adhesive material
title_fullStr	Reciprocation of micro-objects by contraction and extension of Vorticella convallaria using polylysine as adhesive material
title_full_unstemmed	Reciprocation of micro-objects by contraction and extension of Vorticella convallaria using polylysine as adhesive material
title_sort	reciprocation of micro-objects by contraction and extension of vorticella convallaria using polylysine as adhesive material
publisher	The Japan Society of Mechanical Engineers
publishDate	2014
url	https://doaj.org/article/cee3b18672ac4317bf657126f9f53844
work_keys_str_mv	AT moetonagai reciprocationofmicroobjectsbycontractionandextensionofvorticellaconvallariausingpolylysineasadhesivematerial AT hiroshiasai reciprocationofmicroobjectsbycontractionandextensionofvorticellaconvallariausingpolylysineasadhesivematerial AT hiroyukifujita reciprocationofmicroobjectsbycontractionandextensionofvorticellaconvallariausingpolylysineasadhesivematerial
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Reciprocation of micro-objects by contraction and extension of Vorticella convallaria using polylysine as adhesive material

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