Simple low dose radiography allows precise lung volume assessment in mice

Abstract X-ray based lung function (XLF) as a planar method uses dramatically less X-ray dose than computed tomography (CT) but so far lacked the ability to relate its parameters to pulmonary air volume. The purpose of this study was to calibrate the functional constituents of XLF that are biomedica...

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Auteurs principaux:	Amara Khan, Andrea Markus, Thomas Rittmann, Jonas Albers, Frauke Alves, Swen Hülsmann, Christian Dullin
Format:	article
Langue:	EN
Publié:	Nature Portfolio 2021
Sujets:	Medicine R Science Q
Accès en ligne:	https://doaj.org/article/ceea1a46388e43879f7803acbb7f1a29
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spelling	oai:doaj.org-article:ceea1a46388e43879f7803acbb7f1a292021-12-02T14:04:39ZSimple low dose radiography allows precise lung volume assessment in mice10.1038/s41598-021-83319-52045-2322https://doaj.org/article/ceea1a46388e43879f7803acbb7f1a292021-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-83319-5https://doaj.org/toc/2045-2322Abstract X-ray based lung function (XLF) as a planar method uses dramatically less X-ray dose than computed tomography (CT) but so far lacked the ability to relate its parameters to pulmonary air volume. The purpose of this study was to calibrate the functional constituents of XLF that are biomedically decipherable and directly comparable to that of micro-CT and whole-body plethysmography (WBP). Here, we developed a unique set-up for simultaneous assessment of lung function and volume using XLF, micro-CT and WBP on healthy mice. Our results reveal a strong correlation of lung volumes obtained from radiographic XLF and micro-CT and demonstrate that XLF is superior to WBP in sensitivity and precision to assess lung volumes. Importantly, XLF measurement uses only a fraction of the radiation dose and acquisition time required for CT. Therefore, the redefined XLF approach is a promising tool for preclinical longitudinal studies with a substantial potential of clinical translation.Amara KhanAndrea MarkusThomas RittmannJonas AlbersFrauke AlvesSwen HülsmannChristian DullinNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Amara Khan Andrea Markus Thomas Rittmann Jonas Albers Frauke Alves Swen Hülsmann Christian Dullin Simple low dose radiography allows precise lung volume assessment in mice
description	Abstract X-ray based lung function (XLF) as a planar method uses dramatically less X-ray dose than computed tomography (CT) but so far lacked the ability to relate its parameters to pulmonary air volume. The purpose of this study was to calibrate the functional constituents of XLF that are biomedically decipherable and directly comparable to that of micro-CT and whole-body plethysmography (WBP). Here, we developed a unique set-up for simultaneous assessment of lung function and volume using XLF, micro-CT and WBP on healthy mice. Our results reveal a strong correlation of lung volumes obtained from radiographic XLF and micro-CT and demonstrate that XLF is superior to WBP in sensitivity and precision to assess lung volumes. Importantly, XLF measurement uses only a fraction of the radiation dose and acquisition time required for CT. Therefore, the redefined XLF approach is a promising tool for preclinical longitudinal studies with a substantial potential of clinical translation.
format	article
author	Amara Khan Andrea Markus Thomas Rittmann Jonas Albers Frauke Alves Swen Hülsmann Christian Dullin
author_facet	Amara Khan Andrea Markus Thomas Rittmann Jonas Albers Frauke Alves Swen Hülsmann Christian Dullin
author_sort	Amara Khan
title	Simple low dose radiography allows precise lung volume assessment in mice
title_short	Simple low dose radiography allows precise lung volume assessment in mice
title_full	Simple low dose radiography allows precise lung volume assessment in mice
title_fullStr	Simple low dose radiography allows precise lung volume assessment in mice
title_full_unstemmed	Simple low dose radiography allows precise lung volume assessment in mice
title_sort	simple low dose radiography allows precise lung volume assessment in mice
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/ceea1a46388e43879f7803acbb7f1a29
work_keys_str_mv	AT amarakhan simplelowdoseradiographyallowspreciselungvolumeassessmentinmice AT andreamarkus simplelowdoseradiographyallowspreciselungvolumeassessmentinmice AT thomasrittmann simplelowdoseradiographyallowspreciselungvolumeassessmentinmice AT jonasalbers simplelowdoseradiographyallowspreciselungvolumeassessmentinmice AT fraukealves simplelowdoseradiographyallowspreciselungvolumeassessmentinmice AT swenhulsmann simplelowdoseradiographyallowspreciselungvolumeassessmentinmice AT christiandullin simplelowdoseradiographyallowspreciselungvolumeassessmentinmice
_version_	1718392050742722560

Simple low dose radiography allows precise lung volume assessment in mice

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