Simple low dose radiography allows precise lung volume assessment in mice

Abstract X-ray based lung function (XLF) as a planar method uses dramatically less X-ray dose than computed tomography (CT) but so far lacked the ability to relate its parameters to pulmonary air volume. The purpose of this study was to calibrate the functional constituents of XLF that are biomedica...

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Autores principales: Amara Khan, Andrea Markus, Thomas Rittmann, Jonas Albers, Frauke Alves, Swen Hülsmann, Christian Dullin
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Lenguaje:EN
Publicado: Nature Portfolio 2021
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Acceso en línea:https://doaj.org/article/ceea1a46388e43879f7803acbb7f1a29
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spelling oai:doaj.org-article:ceea1a46388e43879f7803acbb7f1a292021-12-02T14:04:39ZSimple low dose radiography allows precise lung volume assessment in mice10.1038/s41598-021-83319-52045-2322https://doaj.org/article/ceea1a46388e43879f7803acbb7f1a292021-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-83319-5https://doaj.org/toc/2045-2322Abstract X-ray based lung function (XLF) as a planar method uses dramatically less X-ray dose than computed tomography (CT) but so far lacked the ability to relate its parameters to pulmonary air volume. The purpose of this study was to calibrate the functional constituents of XLF that are biomedically decipherable and directly comparable to that of micro-CT and whole-body plethysmography (WBP). Here, we developed a unique set-up for simultaneous assessment of lung function and volume using XLF, micro-CT and WBP on healthy mice. Our results reveal a strong correlation of lung volumes obtained from radiographic XLF and micro-CT and demonstrate that XLF is superior to WBP in sensitivity and precision to assess lung volumes. Importantly, XLF measurement uses only a fraction of the radiation dose and acquisition time required for CT. Therefore, the redefined XLF approach is a promising tool for preclinical longitudinal studies with a substantial potential of clinical translation.Amara KhanAndrea MarkusThomas RittmannJonas AlbersFrauke AlvesSwen HülsmannChristian DullinNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Amara Khan
Andrea Markus
Thomas Rittmann
Jonas Albers
Frauke Alves
Swen Hülsmann
Christian Dullin
Simple low dose radiography allows precise lung volume assessment in mice
description Abstract X-ray based lung function (XLF) as a planar method uses dramatically less X-ray dose than computed tomography (CT) but so far lacked the ability to relate its parameters to pulmonary air volume. The purpose of this study was to calibrate the functional constituents of XLF that are biomedically decipherable and directly comparable to that of micro-CT and whole-body plethysmography (WBP). Here, we developed a unique set-up for simultaneous assessment of lung function and volume using XLF, micro-CT and WBP on healthy mice. Our results reveal a strong correlation of lung volumes obtained from radiographic XLF and micro-CT and demonstrate that XLF is superior to WBP in sensitivity and precision to assess lung volumes. Importantly, XLF measurement uses only a fraction of the radiation dose and acquisition time required for CT. Therefore, the redefined XLF approach is a promising tool for preclinical longitudinal studies with a substantial potential of clinical translation.
format article
author Amara Khan
Andrea Markus
Thomas Rittmann
Jonas Albers
Frauke Alves
Swen Hülsmann
Christian Dullin
author_facet Amara Khan
Andrea Markus
Thomas Rittmann
Jonas Albers
Frauke Alves
Swen Hülsmann
Christian Dullin
author_sort Amara Khan
title Simple low dose radiography allows precise lung volume assessment in mice
title_short Simple low dose radiography allows precise lung volume assessment in mice
title_full Simple low dose radiography allows precise lung volume assessment in mice
title_fullStr Simple low dose radiography allows precise lung volume assessment in mice
title_full_unstemmed Simple low dose radiography allows precise lung volume assessment in mice
title_sort simple low dose radiography allows precise lung volume assessment in mice
publisher Nature Portfolio
publishDate 2021
url https://doaj.org/article/ceea1a46388e43879f7803acbb7f1a29
work_keys_str_mv AT amarakhan simplelowdoseradiographyallowspreciselungvolumeassessmentinmice
AT andreamarkus simplelowdoseradiographyallowspreciselungvolumeassessmentinmice
AT thomasrittmann simplelowdoseradiographyallowspreciselungvolumeassessmentinmice
AT jonasalbers simplelowdoseradiographyallowspreciselungvolumeassessmentinmice
AT fraukealves simplelowdoseradiographyallowspreciselungvolumeassessmentinmice
AT swenhulsmann simplelowdoseradiographyallowspreciselungvolumeassessmentinmice
AT christiandullin simplelowdoseradiographyallowspreciselungvolumeassessmentinmice
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