Urinary microRNAs as non-invasive biomarkers for toxic acute kidney injury in humans

Abstract MicroRNAs in biofluids are potential biomarkers for detecting kidney and other organ injuries. We profiled microRNAs in urine samples from patients with Russell’s viper envenoming or acute self-poisoning following paraquat, glyphosate, or oxalic acid [with and without acute kidney injury (A...

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Autores principales:	Fathima Shihana, Wilson K. M. Wong, Mugdha V. Joglekar, Fahim Mohamed, Indika B. Gawarammana, Geoffrey K. Isbister, Anandwardhan A. Hardikar, Devanshi Seth, Nicholas A. Buckley
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/ceee17a02abe49339b2390610baa4e8d
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spelling	oai:doaj.org-article:ceee17a02abe49339b2390610baa4e8d2021-12-02T17:39:31ZUrinary microRNAs as non-invasive biomarkers for toxic acute kidney injury in humans10.1038/s41598-021-87918-02045-2322https://doaj.org/article/ceee17a02abe49339b2390610baa4e8d2021-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-87918-0https://doaj.org/toc/2045-2322Abstract MicroRNAs in biofluids are potential biomarkers for detecting kidney and other organ injuries. We profiled microRNAs in urine samples from patients with Russell’s viper envenoming or acute self-poisoning following paraquat, glyphosate, or oxalic acid [with and without acute kidney injury (AKI)] and on healthy controls. Discovery analysis profiled for 754 microRNAs using TaqMan OpenArray qPCR with three patients per group (12 samples in each toxic agent). From these, 53 microRNAs were selected and validated in a larger cohort of patients (Russell’s viper envenoming = 53, paraquat = 51, glyphosate = 51, oxalic acid = 40) and 27 healthy controls. Urinary microRNAs had significantly higher expression in patients poisoned/envenomed by different nephrotoxic agents in both discovery and validation cohorts. Seven microRNAs discriminated severe AKI patients from no AKI for all four nephrotoxic agents. Four microRNAs (miR-30a-3p, miR-30a-5p, miR-92a, and miR-204) had > 17 fold change (p < 0.0001) and receiver operator characteristics area-under-curve (ROC-AUC) > 0.72. Pathway analysis of target mRNAs of these differentially expressed microRNAs showed association with the regulation of different nephrotoxic signaling pathways. In conclusion, human urinary microRNAs could identify toxic AKI early after acute injury. These urinary microRNAs have potential clinical application as early non-invasive diagnostic AKI biomarkers.Fathima ShihanaWilson K. M. WongMugdha V. JoglekarFahim MohamedIndika B. GawarammanaGeoffrey K. IsbisterAnandwardhan A. HardikarDevanshi SethNicholas A. BuckleyNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Fathima Shihana Wilson K. M. Wong Mugdha V. Joglekar Fahim Mohamed Indika B. Gawarammana Geoffrey K. Isbister Anandwardhan A. Hardikar Devanshi Seth Nicholas A. Buckley Urinary microRNAs as non-invasive biomarkers for toxic acute kidney injury in humans
description	Abstract MicroRNAs in biofluids are potential biomarkers for detecting kidney and other organ injuries. We profiled microRNAs in urine samples from patients with Russell’s viper envenoming or acute self-poisoning following paraquat, glyphosate, or oxalic acid [with and without acute kidney injury (AKI)] and on healthy controls. Discovery analysis profiled for 754 microRNAs using TaqMan OpenArray qPCR with three patients per group (12 samples in each toxic agent). From these, 53 microRNAs were selected and validated in a larger cohort of patients (Russell’s viper envenoming = 53, paraquat = 51, glyphosate = 51, oxalic acid = 40) and 27 healthy controls. Urinary microRNAs had significantly higher expression in patients poisoned/envenomed by different nephrotoxic agents in both discovery and validation cohorts. Seven microRNAs discriminated severe AKI patients from no AKI for all four nephrotoxic agents. Four microRNAs (miR-30a-3p, miR-30a-5p, miR-92a, and miR-204) had > 17 fold change (p < 0.0001) and receiver operator characteristics area-under-curve (ROC-AUC) > 0.72. Pathway analysis of target mRNAs of these differentially expressed microRNAs showed association with the regulation of different nephrotoxic signaling pathways. In conclusion, human urinary microRNAs could identify toxic AKI early after acute injury. These urinary microRNAs have potential clinical application as early non-invasive diagnostic AKI biomarkers.
format	article
author	Fathima Shihana Wilson K. M. Wong Mugdha V. Joglekar Fahim Mohamed Indika B. Gawarammana Geoffrey K. Isbister Anandwardhan A. Hardikar Devanshi Seth Nicholas A. Buckley
author_facet	Fathima Shihana Wilson K. M. Wong Mugdha V. Joglekar Fahim Mohamed Indika B. Gawarammana Geoffrey K. Isbister Anandwardhan A. Hardikar Devanshi Seth Nicholas A. Buckley
author_sort	Fathima Shihana
title	Urinary microRNAs as non-invasive biomarkers for toxic acute kidney injury in humans
title_short	Urinary microRNAs as non-invasive biomarkers for toxic acute kidney injury in humans
title_full	Urinary microRNAs as non-invasive biomarkers for toxic acute kidney injury in humans
title_fullStr	Urinary microRNAs as non-invasive biomarkers for toxic acute kidney injury in humans
title_full_unstemmed	Urinary microRNAs as non-invasive biomarkers for toxic acute kidney injury in humans
title_sort	urinary micrornas as non-invasive biomarkers for toxic acute kidney injury in humans
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/ceee17a02abe49339b2390610baa4e8d
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Urinary microRNAs as non-invasive biomarkers for toxic acute kidney injury in humans

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