In Vivo Healthy Benefits of Galacto-Oligosaccharides from <i>Lupinus albus</i> (LA-GOS) in Butyrate Production through Intestinal Microbiota

Animal digestive systems host microorganism ecosystems, including integrated bacteria, viruses, fungi, and others, that produce a variety of compounds from different substrates with healthy properties. Among these substrates, α-galacto-oligosaccharides (GOS) are considered prebiotics that promote th...

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Autores principales:	Lucila A. Godínez-Méndez, Carmen M. Gurrola-Díaz, José Sergio Zepeda-Nuño, Natali Vega-Magaña, Rocio Ivette Lopez-Roa, Liliana Íñiguez-Gutiérrez, Pedro M. García-López, Mary Fafutis-Morris, Vidal Delgado-Rizo
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	<i>Lupinus albus</i> short chain fatty acids ulcerative colitis intestinal microbiota dysbiosis goblet cells Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/cef07996c1fb4737b68e2320ee4f9222
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spelling	oai:doaj.org-article:cef07996c1fb4737b68e2320ee4f92222021-11-25T16:53:27ZIn Vivo Healthy Benefits of Galacto-Oligosaccharides from <i>Lupinus albus</i> (LA-GOS) in Butyrate Production through Intestinal Microbiota10.3390/biom111116582218-273Xhttps://doaj.org/article/cef07996c1fb4737b68e2320ee4f92222021-11-01T00:00:00Zhttps://www.mdpi.com/2218-273X/11/11/1658https://doaj.org/toc/2218-273XAnimal digestive systems host microorganism ecosystems, including integrated bacteria, viruses, fungi, and others, that produce a variety of compounds from different substrates with healthy properties. Among these substrates, α-galacto-oligosaccharides (GOS) are considered prebiotics that promote the grow of gut microbiota with a metabolic output of Short Chain Fatty Acids (SCFAs). In this regard, we evaluated <i>Lupinus albus</i> GOS (LA-GOS) as a natural prebiotic using different animal models. Therefore, the aim of this work was to evaluate the effect of LA-GOS on the gut microbiota, SCFA production, and intestinal health in healthy and induced dysbiosis conditions (an ulcerative colitis (UC) model). Twenty C57BL/6 mice were randomly allocated in four groups (<i>n</i> = 5/group): untreated and treated non-induced animals, and two groups induced with 2% dextran sulfate sodium to UC with and without LA-GOS administration (2.5 g/kg bw). We found that the UC treated group showed a higher goblet cell number, lower disease activity index, and reduced histopathological damage in comparison to the UC untreated group. In addition, the abundance of positive bacteria to butyryl-CoA transferase in gut microbiota was significantly increased by LA-GOS treatment, in healthy conditions. We measured the SCFA production with significant differences in the butyrate concentration between treated and untreated healthy groups. Finally, the pH level in cecum feces was reduced after LA-GOS treatment. Overall, we point out the in vivo health benefits of LA-GOS administration on the preservation of the intestinal ecosystem and the promotion of SCFA production.Lucila A. Godínez-MéndezCarmen M. Gurrola-DíazJosé Sergio Zepeda-NuñoNatali Vega-MagañaRocio Ivette Lopez-RoaLiliana Íñiguez-GutiérrezPedro M. García-LópezMary Fafutis-MorrisVidal Delgado-RizoMDPI AGarticle<i>Lupinus albus</i>short chain fatty acidsulcerative colitisintestinal microbiotadysbiosisgoblet cellsMicrobiologyQR1-502ENBiomolecules, Vol 11, Iss 1658, p 1658 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	<i>Lupinus albus</i> short chain fatty acids ulcerative colitis intestinal microbiota dysbiosis goblet cells Microbiology QR1-502
spellingShingle	<i>Lupinus albus</i> short chain fatty acids ulcerative colitis intestinal microbiota dysbiosis goblet cells Microbiology QR1-502 Lucila A. Godínez-Méndez Carmen M. Gurrola-Díaz José Sergio Zepeda-Nuño Natali Vega-Magaña Rocio Ivette Lopez-Roa Liliana Íñiguez-Gutiérrez Pedro M. García-López Mary Fafutis-Morris Vidal Delgado-Rizo In Vivo Healthy Benefits of Galacto-Oligosaccharides from <i>Lupinus albus</i> (LA-GOS) in Butyrate Production through Intestinal Microbiota
description	Animal digestive systems host microorganism ecosystems, including integrated bacteria, viruses, fungi, and others, that produce a variety of compounds from different substrates with healthy properties. Among these substrates, α-galacto-oligosaccharides (GOS) are considered prebiotics that promote the grow of gut microbiota with a metabolic output of Short Chain Fatty Acids (SCFAs). In this regard, we evaluated <i>Lupinus albus</i> GOS (LA-GOS) as a natural prebiotic using different animal models. Therefore, the aim of this work was to evaluate the effect of LA-GOS on the gut microbiota, SCFA production, and intestinal health in healthy and induced dysbiosis conditions (an ulcerative colitis (UC) model). Twenty C57BL/6 mice were randomly allocated in four groups (<i>n</i> = 5/group): untreated and treated non-induced animals, and two groups induced with 2% dextran sulfate sodium to UC with and without LA-GOS administration (2.5 g/kg bw). We found that the UC treated group showed a higher goblet cell number, lower disease activity index, and reduced histopathological damage in comparison to the UC untreated group. In addition, the abundance of positive bacteria to butyryl-CoA transferase in gut microbiota was significantly increased by LA-GOS treatment, in healthy conditions. We measured the SCFA production with significant differences in the butyrate concentration between treated and untreated healthy groups. Finally, the pH level in cecum feces was reduced after LA-GOS treatment. Overall, we point out the in vivo health benefits of LA-GOS administration on the preservation of the intestinal ecosystem and the promotion of SCFA production.
format	article
author	Lucila A. Godínez-Méndez Carmen M. Gurrola-Díaz José Sergio Zepeda-Nuño Natali Vega-Magaña Rocio Ivette Lopez-Roa Liliana Íñiguez-Gutiérrez Pedro M. García-López Mary Fafutis-Morris Vidal Delgado-Rizo
author_facet	Lucila A. Godínez-Méndez Carmen M. Gurrola-Díaz José Sergio Zepeda-Nuño Natali Vega-Magaña Rocio Ivette Lopez-Roa Liliana Íñiguez-Gutiérrez Pedro M. García-López Mary Fafutis-Morris Vidal Delgado-Rizo
author_sort	Lucila A. Godínez-Méndez
title	In Vivo Healthy Benefits of Galacto-Oligosaccharides from <i>Lupinus albus</i> (LA-GOS) in Butyrate Production through Intestinal Microbiota
title_short	In Vivo Healthy Benefits of Galacto-Oligosaccharides from <i>Lupinus albus</i> (LA-GOS) in Butyrate Production through Intestinal Microbiota
title_full	In Vivo Healthy Benefits of Galacto-Oligosaccharides from <i>Lupinus albus</i> (LA-GOS) in Butyrate Production through Intestinal Microbiota
title_fullStr	In Vivo Healthy Benefits of Galacto-Oligosaccharides from <i>Lupinus albus</i> (LA-GOS) in Butyrate Production through Intestinal Microbiota
title_full_unstemmed	In Vivo Healthy Benefits of Galacto-Oligosaccharides from <i>Lupinus albus</i> (LA-GOS) in Butyrate Production through Intestinal Microbiota
title_sort	in vivo healthy benefits of galacto-oligosaccharides from <i>lupinus albus</i> (la-gos) in butyrate production through intestinal microbiota
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/cef07996c1fb4737b68e2320ee4f9222
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In Vivo Healthy Benefits of Galacto-Oligosaccharides from <i>Lupinus albus</i> (LA-GOS) in Butyrate Production through Intestinal Microbiota

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