COMPARATIVE STUDY OF “KLIM-TEST” MONOCLONAL ANTIBODIES FOR PHENOTYPING OF PERIPHERAL BLOOD CELLS WITH MONOCLONALS FROM FOREIGN PRODUCERS

In view of wide-spread usage of flow cytometry (FC) in various areas of modern medicine, Russian market is still dominated by foreign manufacturers of monoclonal antibodies (MAbs). This problem is accomplished by lack of information about Russian producers of MAbs. Objective: to compare a set of FC...

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Autores principales:	A. A. Popova, L. V. Serebrovskaya, L. A. Ivanova, O. N. Khokhlova, S. N. Novozhilov
Formato:	article
Lenguaje:	RU
Publicado:	SPb RAACI 2014
Materias:	monoclonal antibodies cd45 gating double-platform approach Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/cefe175f91ba45909a8c831b9ebda84e
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spelling	oai:doaj.org-article:cefe175f91ba45909a8c831b9ebda84e2021-11-18T08:03:38ZCOMPARATIVE STUDY OF “KLIM-TEST” MONOCLONAL ANTIBODIES FOR PHENOTYPING OF PERIPHERAL BLOOD CELLS WITH MONOCLONALS FROM FOREIGN PRODUCERS1563-06252313-741X10.15789/1563-0625-2013-2-177-184https://doaj.org/article/cefe175f91ba45909a8c831b9ebda84e2014-07-01T00:00:00Zhttps://www.mimmun.ru/mimmun/article/view/136https://doaj.org/toc/1563-0625https://doaj.org/toc/2313-741XIn view of wide-spread usage of flow cytometry (FC) in various areas of modern medicine, Russian market is still dominated by foreign manufacturers of monoclonal antibodies (MAbs). This problem is accomplished by lack of information about Russian producers of MAbs. Objective: to compare a set of FC reagents produced by a Russian manufacturer with MAbs for FC from different foreign manufacturers. Venous blood served as biomaterial for this study. The parameters under study were measured by FC techniques. Comparative immunophenotyping was performed with MAbs from Becton Dickinson (USA), Lab Constant (Russia), and Beckman Coulter (USA). Virtually complete absence of systematic differences and a relatively small spread of values was revealed by means of Bland–Altman analysis. The values of parameters, as measured with different MAbs, did not differ from one another by significance level of 0.01, according to Friedman criterion. The following evidence has been yielded in present study: (1) data obtained with “Klim-Test” reagents do not exhidit any systematic differences, as compared with Mabs from foreign manufacturers, and they show a relatively small scatter of results. “Klim-Test” reagents may be applied for Becton Dickinson and Beckman Coulter flow cytometers. (2) These reagents are equally effective at the levels of CD4-lymphocytes above 500 cells/mcL, and below these values. (3) Usage of “Klim-Test” reagents with a three-color label for CD45 cells (CD3-FITC/CD4-PE/CD45-PE-Sy5) corresponds to the standards for CD4-lymphocyte count in HIV-infected patients, thus allowing it to recommend this reagent for HIV-infection monitoring.A. A. PopovaL. V. SerebrovskayaL. A. IvanovaO. N. KhokhlovaS. N. NovozhilovSPb RAACIarticlemonoclonal antibodiescd45 gatingdouble-platform approachImmunologic diseases. AllergyRC581-607RUMedicinskaâ Immunologiâ, Vol 15, Iss 2, Pp 177-184 (2014)
institution	DOAJ
collection	DOAJ
language	RU
topic	monoclonal antibodies cd45 gating double-platform approach Immunologic diseases. Allergy RC581-607
spellingShingle	monoclonal antibodies cd45 gating double-platform approach Immunologic diseases. Allergy RC581-607 A. A. Popova L. V. Serebrovskaya L. A. Ivanova O. N. Khokhlova S. N. Novozhilov COMPARATIVE STUDY OF “KLIM-TEST” MONOCLONAL ANTIBODIES FOR PHENOTYPING OF PERIPHERAL BLOOD CELLS WITH MONOCLONALS FROM FOREIGN PRODUCERS
description	In view of wide-spread usage of flow cytometry (FC) in various areas of modern medicine, Russian market is still dominated by foreign manufacturers of monoclonal antibodies (MAbs). This problem is accomplished by lack of information about Russian producers of MAbs. Objective: to compare a set of FC reagents produced by a Russian manufacturer with MAbs for FC from different foreign manufacturers. Venous blood served as biomaterial for this study. The parameters under study were measured by FC techniques. Comparative immunophenotyping was performed with MAbs from Becton Dickinson (USA), Lab Constant (Russia), and Beckman Coulter (USA). Virtually complete absence of systematic differences and a relatively small spread of values was revealed by means of Bland–Altman analysis. The values of parameters, as measured with different MAbs, did not differ from one another by significance level of 0.01, according to Friedman criterion. The following evidence has been yielded in present study: (1) data obtained with “Klim-Test” reagents do not exhidit any systematic differences, as compared with Mabs from foreign manufacturers, and they show a relatively small scatter of results. “Klim-Test” reagents may be applied for Becton Dickinson and Beckman Coulter flow cytometers. (2) These reagents are equally effective at the levels of CD4-lymphocytes above 500 cells/mcL, and below these values. (3) Usage of “Klim-Test” reagents with a three-color label for CD45 cells (CD3-FITC/CD4-PE/CD45-PE-Sy5) corresponds to the standards for CD4-lymphocyte count in HIV-infected patients, thus allowing it to recommend this reagent for HIV-infection monitoring.
format	article
author	A. A. Popova L. V. Serebrovskaya L. A. Ivanova O. N. Khokhlova S. N. Novozhilov
author_facet	A. A. Popova L. V. Serebrovskaya L. A. Ivanova O. N. Khokhlova S. N. Novozhilov
author_sort	A. A. Popova
title	COMPARATIVE STUDY OF “KLIM-TEST” MONOCLONAL ANTIBODIES FOR PHENOTYPING OF PERIPHERAL BLOOD CELLS WITH MONOCLONALS FROM FOREIGN PRODUCERS
title_short	COMPARATIVE STUDY OF “KLIM-TEST” MONOCLONAL ANTIBODIES FOR PHENOTYPING OF PERIPHERAL BLOOD CELLS WITH MONOCLONALS FROM FOREIGN PRODUCERS
title_full	COMPARATIVE STUDY OF “KLIM-TEST” MONOCLONAL ANTIBODIES FOR PHENOTYPING OF PERIPHERAL BLOOD CELLS WITH MONOCLONALS FROM FOREIGN PRODUCERS
title_fullStr	COMPARATIVE STUDY OF “KLIM-TEST” MONOCLONAL ANTIBODIES FOR PHENOTYPING OF PERIPHERAL BLOOD CELLS WITH MONOCLONALS FROM FOREIGN PRODUCERS
title_full_unstemmed	COMPARATIVE STUDY OF “KLIM-TEST” MONOCLONAL ANTIBODIES FOR PHENOTYPING OF PERIPHERAL BLOOD CELLS WITH MONOCLONALS FROM FOREIGN PRODUCERS
title_sort	comparative study of “klim-test” monoclonal antibodies for phenotyping of peripheral blood cells with monoclonals from foreign producers
publisher	SPb RAACI
publishDate	2014
url	https://doaj.org/article/cefe175f91ba45909a8c831b9ebda84e
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COMPARATIVE STUDY OF “KLIM-TEST” MONOCLONAL ANTIBODIES FOR PHENOTYPING OF PERIPHERAL BLOOD CELLS WITH MONOCLONALS FROM FOREIGN PRODUCERS

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