Selection of aptamers for mature white adipocytes by cell SELEX using flow cytometry.

<h4>Background</h4>Adipose tissue, mainly composed of adipocytes, plays an important role in metabolism by regulating energy homeostasis. Obesity is primarily caused by an abundance of adipose tissue. Therefore, specific targeting of adipose tissue is critical during the treatment of obe...

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Autores principales:	Eun Young Kim, Ji Won Kim, Won Kon Kim, Baek Soo Han, Sung Goo Park, Bong Hyun Chung, Sang Chul Lee, Kwang-Hee Bae
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/cf07434be5b242c89f28e50c5d0a5578
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spelling	oai:doaj.org-article:cf07434be5b242c89f28e50c5d0a55782021-11-18T08:18:39ZSelection of aptamers for mature white adipocytes by cell SELEX using flow cytometry.1932-620310.1371/journal.pone.0097747https://doaj.org/article/cf07434be5b242c89f28e50c5d0a55782014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24844710/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>Adipose tissue, mainly composed of adipocytes, plays an important role in metabolism by regulating energy homeostasis. Obesity is primarily caused by an abundance of adipose tissue. Therefore, specific targeting of adipose tissue is critical during the treatment of obesity, and plays a major role in overcoming it. However, the knowledge of cell-surface markers specific to adipocytes is limited.<h4>Methods and results</h4>We applied the CELL SELEX (Systematic Evolution of Ligands by EXponential enrichment) method using flow cytometry to isolate molecular probes for specific recognition of adipocytes. The aptamer library, a mixture of FITC-tagged single-stranded random DNAs, is used as a source for acquiring molecular probes. With the increasing number of selection cycles, there was a steady increase in the fluorescence intensity toward mature adipocytes. Through 12 rounds of SELEX, enriched aptamers showing specific recognition toward mature 3T3-L1 adipocyte cells were isolated. Among these, two aptamers (MA-33 and 91) were able to selectively bind to mature adipocytes with an equilibrium dissociation constant (Kd) in the nanomolar range. These aptamers did not bind to preadipocytes or other cell lines (such as HeLa, HEK-293, or C2C12 cells). Additionally, it was confirmed that MA-33 and 91 can distinguish between mature primary white and primary brown adipocytes.<h4>Conclusions</h4>These selected aptamers have the potential to be applied as markers for detecting mature white adipocytes and monitoring adipogenesis, and could emerge as an important tool in the treatment of obesity.Eun Young KimJi Won KimWon Kon KimBaek Soo HanSung Goo ParkBong Hyun ChungSang Chul LeeKwang-Hee BaePublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 5, p e97747 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Eun Young Kim Ji Won Kim Won Kon Kim Baek Soo Han Sung Goo Park Bong Hyun Chung Sang Chul Lee Kwang-Hee Bae Selection of aptamers for mature white adipocytes by cell SELEX using flow cytometry.
description	<h4>Background</h4>Adipose tissue, mainly composed of adipocytes, plays an important role in metabolism by regulating energy homeostasis. Obesity is primarily caused by an abundance of adipose tissue. Therefore, specific targeting of adipose tissue is critical during the treatment of obesity, and plays a major role in overcoming it. However, the knowledge of cell-surface markers specific to adipocytes is limited.<h4>Methods and results</h4>We applied the CELL SELEX (Systematic Evolution of Ligands by EXponential enrichment) method using flow cytometry to isolate molecular probes for specific recognition of adipocytes. The aptamer library, a mixture of FITC-tagged single-stranded random DNAs, is used as a source for acquiring molecular probes. With the increasing number of selection cycles, there was a steady increase in the fluorescence intensity toward mature adipocytes. Through 12 rounds of SELEX, enriched aptamers showing specific recognition toward mature 3T3-L1 adipocyte cells were isolated. Among these, two aptamers (MA-33 and 91) were able to selectively bind to mature adipocytes with an equilibrium dissociation constant (Kd) in the nanomolar range. These aptamers did not bind to preadipocytes or other cell lines (such as HeLa, HEK-293, or C2C12 cells). Additionally, it was confirmed that MA-33 and 91 can distinguish between mature primary white and primary brown adipocytes.<h4>Conclusions</h4>These selected aptamers have the potential to be applied as markers for detecting mature white adipocytes and monitoring adipogenesis, and could emerge as an important tool in the treatment of obesity.
format	article
author	Eun Young Kim Ji Won Kim Won Kon Kim Baek Soo Han Sung Goo Park Bong Hyun Chung Sang Chul Lee Kwang-Hee Bae
author_facet	Eun Young Kim Ji Won Kim Won Kon Kim Baek Soo Han Sung Goo Park Bong Hyun Chung Sang Chul Lee Kwang-Hee Bae
author_sort	Eun Young Kim
title	Selection of aptamers for mature white adipocytes by cell SELEX using flow cytometry.
title_short	Selection of aptamers for mature white adipocytes by cell SELEX using flow cytometry.
title_full	Selection of aptamers for mature white adipocytes by cell SELEX using flow cytometry.
title_fullStr	Selection of aptamers for mature white adipocytes by cell SELEX using flow cytometry.
title_full_unstemmed	Selection of aptamers for mature white adipocytes by cell SELEX using flow cytometry.
title_sort	selection of aptamers for mature white adipocytes by cell selex using flow cytometry.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/cf07434be5b242c89f28e50c5d0a5578
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Selection of aptamers for mature white adipocytes by cell SELEX using flow cytometry.

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