Platelet Mediated Inflammation in Coronary Artery Disease with Type 2 Diabetes Patients

Ebin Johny,1 Pathoori Bhaskar,1 Md Jahangir Alam,2 Indra Kuladhipati,3 Rupam Das,3 Ramu Adela1 1Department of Pharmacy Practice, National Institute of Pharmaceutical Education and Research (NIPER)-Guwahati, Guwahati, Assam, 781101, India; 2Department of Biotechnology, National Institute of Pharmaceu...

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Autores principales:	Johny E, Bhaskar P, Alam MJ, Kuladhipati I, Das R, Adela R
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2021
Materias:	platelet inflammation monocytes hyperglycemia type 2 diabetes coronary artery disease Pathology RB1-214 Therapeutics. Pharmacology RM1-950
Acceso en línea:	https://doaj.org/article/cf08f6f612b349b3a2d09deca46d25cd
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spelling	oai:doaj.org-article:cf08f6f612b349b3a2d09deca46d25cd2021-12-02T18:07:10ZPlatelet Mediated Inflammation in Coronary Artery Disease with Type 2 Diabetes Patients1178-7031https://doaj.org/article/cf08f6f612b349b3a2d09deca46d25cd2021-10-01T00:00:00Zhttps://www.dovepress.com/platelet-mediated-inflammation-in-coronary-artery-disease-with-type-2--peer-reviewed-fulltext-article-JIRhttps://doaj.org/toc/1178-7031Ebin Johny,1 Pathoori Bhaskar,1 Md Jahangir Alam,2 Indra Kuladhipati,3 Rupam Das,3 Ramu Adela1 1Department of Pharmacy Practice, National Institute of Pharmaceutical Education and Research (NIPER)-Guwahati, Guwahati, Assam, 781101, India; 2Department of Biotechnology, National Institute of Pharmaceutical Education and Research (NIPER)-Guwahati, Guwahati, Assam, 781101, India; 3Down Town Hospital, Guwahati, Assam, 781006, IndiaCorrespondence: Ramu AdelaDepartment of Pharmacy Practice, National Institute of Pharmaceutical Education and Research (NIPER)-Guwahati, Sila Katamur (Halugurisuk), Changsari, Kamrup, Guwahati, Assam, 781101, IndiaTel +91 8860280242Email ramuadela@gmail.comBackground: Type 2 diabetes mellitus (T2DM) is a well-established risk factor for the development of atherosclerotic coronary artery disease. Platelet hyperactivity and inflammation are associated with the development of coronary artery disease (CAD) in T2DM patients. We investigated the status of immune cells, platelet activation, and platelet-immune cell interactions in T2DM_CAD patients.Methodology: The study population consisted of four groups of subjects, healthy control (CT, n = 20), T2DM (n = 44), CAD (n = 20) and T2DM_CAD (n = 38). Platelet activation, immunome profiling and platelet-immune cell interactions were analysed by flow cytometry. The circulatory levels of inflammatory cytokines/chemokines were assessed using multiplex assay.Results: Increased platelet activation and increased platelet-immune cell aggregate formation were observed in T2DM and T2DM_CAD groups compared to the control and CAD groups (p < 0.05). Our immunome profile analysis revealed, altered monocyte subpopulations and dendritic cell populations in T2DM, CAD and T2DM_CAD groups compared to the control group (p < 0.05). Furthermore, significantly increased IL-1β, IL-2, IL-4, IL-6, IL-8, IL12p70, IL-13 IL-18, CCL2, and decreased CXCL1, CCL5 levels were observed in T2DM_CAD group compared to the control group. Our ex-vivo study increased platelet-monocyte aggregate formation was observed upon D-glucose exposure in a time and concentration dependent manner.Conclusion: Our data suggests that T2DM, CAD and T2DM_CAD are associated with altered immune cell populations. Furthermore, it has been confirmed that hyperglycemia induces platelet activation and forms platelet-immune cell aggregation which may lead to the release of inflammatory cytokines and chemokines and contribute to the complexity of CAD and type 2 diabetes.Keywords: platelet, inflammation, monocytes, hyperglycemia, type 2 diabetes, coronary artery diseaseJohny EBhaskar PAlam MJKuladhipati IDas RAdela RDove Medical Pressarticleplateletinflammationmonocyteshyperglycemiatype 2 diabetescoronary artery diseasePathologyRB1-214Therapeutics. PharmacologyRM1-950ENJournal of Inflammation Research, Vol Volume 14, Pp 5131-5147 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	platelet inflammation monocytes hyperglycemia type 2 diabetes coronary artery disease Pathology RB1-214 Therapeutics. Pharmacology RM1-950
spellingShingle	platelet inflammation monocytes hyperglycemia type 2 diabetes coronary artery disease Pathology RB1-214 Therapeutics. Pharmacology RM1-950 Johny E Bhaskar P Alam MJ Kuladhipati I Das R Adela R Platelet Mediated Inflammation in Coronary Artery Disease with Type 2 Diabetes Patients
description	Ebin Johny,1 Pathoori Bhaskar,1 Md Jahangir Alam,2 Indra Kuladhipati,3 Rupam Das,3 Ramu Adela1 1Department of Pharmacy Practice, National Institute of Pharmaceutical Education and Research (NIPER)-Guwahati, Guwahati, Assam, 781101, India; 2Department of Biotechnology, National Institute of Pharmaceutical Education and Research (NIPER)-Guwahati, Guwahati, Assam, 781101, India; 3Down Town Hospital, Guwahati, Assam, 781006, IndiaCorrespondence: Ramu AdelaDepartment of Pharmacy Practice, National Institute of Pharmaceutical Education and Research (NIPER)-Guwahati, Sila Katamur (Halugurisuk), Changsari, Kamrup, Guwahati, Assam, 781101, IndiaTel +91 8860280242Email ramuadela@gmail.comBackground: Type 2 diabetes mellitus (T2DM) is a well-established risk factor for the development of atherosclerotic coronary artery disease. Platelet hyperactivity and inflammation are associated with the development of coronary artery disease (CAD) in T2DM patients. We investigated the status of immune cells, platelet activation, and platelet-immune cell interactions in T2DM_CAD patients.Methodology: The study population consisted of four groups of subjects, healthy control (CT, n = 20), T2DM (n = 44), CAD (n = 20) and T2DM_CAD (n = 38). Platelet activation, immunome profiling and platelet-immune cell interactions were analysed by flow cytometry. The circulatory levels of inflammatory cytokines/chemokines were assessed using multiplex assay.Results: Increased platelet activation and increased platelet-immune cell aggregate formation were observed in T2DM and T2DM_CAD groups compared to the control and CAD groups (p < 0.05). Our immunome profile analysis revealed, altered monocyte subpopulations and dendritic cell populations in T2DM, CAD and T2DM_CAD groups compared to the control group (p < 0.05). Furthermore, significantly increased IL-1β, IL-2, IL-4, IL-6, IL-8, IL12p70, IL-13 IL-18, CCL2, and decreased CXCL1, CCL5 levels were observed in T2DM_CAD group compared to the control group. Our ex-vivo study increased platelet-monocyte aggregate formation was observed upon D-glucose exposure in a time and concentration dependent manner.Conclusion: Our data suggests that T2DM, CAD and T2DM_CAD are associated with altered immune cell populations. Furthermore, it has been confirmed that hyperglycemia induces platelet activation and forms platelet-immune cell aggregation which may lead to the release of inflammatory cytokines and chemokines and contribute to the complexity of CAD and type 2 diabetes.Keywords: platelet, inflammation, monocytes, hyperglycemia, type 2 diabetes, coronary artery disease
format	article
author	Johny E Bhaskar P Alam MJ Kuladhipati I Das R Adela R
author_facet	Johny E Bhaskar P Alam MJ Kuladhipati I Das R Adela R
author_sort	Johny E
title	Platelet Mediated Inflammation in Coronary Artery Disease with Type 2 Diabetes Patients
title_short	Platelet Mediated Inflammation in Coronary Artery Disease with Type 2 Diabetes Patients
title_full	Platelet Mediated Inflammation in Coronary Artery Disease with Type 2 Diabetes Patients
title_fullStr	Platelet Mediated Inflammation in Coronary Artery Disease with Type 2 Diabetes Patients
title_full_unstemmed	Platelet Mediated Inflammation in Coronary Artery Disease with Type 2 Diabetes Patients
title_sort	platelet mediated inflammation in coronary artery disease with type 2 diabetes patients
publisher	Dove Medical Press
publishDate	2021
url	https://doaj.org/article/cf08f6f612b349b3a2d09deca46d25cd
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Platelet Mediated Inflammation in Coronary Artery Disease with Type 2 Diabetes Patients

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