Cytoprotective Peptides from Blue Mussel Protein Hydrolysates: Identification and Mechanism Investigation in Human Umbilical Vein Endothelial Cells Injury

Cardiovascular disease represents a leading cause of mortality and is often characterized by the emergence of endothelial dysfunction (ED), a physiologic condition that takes place in the early progress of atherosclerosis. In this study, two cytoprotective peptides derived from blue mussel chymotryp...

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Autores principales:	Indyaswan Tegar Suryaningtyas, Chang-Bum Ahn, Jae-Young Je
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	bioactive peptide cytoprotective oxidative stress endothelial dysfunction blue mussel Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/cf1175adf48c43a1b887c972c28b50bc
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spelling	oai:doaj.org-article:cf1175adf48c43a1b887c972c28b50bc2021-11-25T18:12:47ZCytoprotective Peptides from Blue Mussel Protein Hydrolysates: Identification and Mechanism Investigation in Human Umbilical Vein Endothelial Cells Injury10.3390/md191106091660-3397https://doaj.org/article/cf1175adf48c43a1b887c972c28b50bc2021-10-01T00:00:00Zhttps://www.mdpi.com/1660-3397/19/11/609https://doaj.org/toc/1660-3397Cardiovascular disease represents a leading cause of mortality and is often characterized by the emergence of endothelial dysfunction (ED), a physiologic condition that takes place in the early progress of atherosclerosis. In this study, two cytoprotective peptides derived from blue mussel chymotrypsin hydrolysates with the sequence of EPTF and FTVN were purified and identified. Molecular mechanisms underlying the cytoprotective effects against oxidative stress which lead to human umbilical vein endothelial cells (HUVEC) injury were investigated. The results showed that pretreatment of EPTF, FTVN and their combination (1:1) in 0.1 mg/mL significantly reduced HUVEC death due to H<sub>2</sub>O<sub>2</sub> exposure. The cytoprotective mechanism of these peptides involves an improvement in the cellular antioxidant defense system, as indicated by the suppression of the intracellular ROS generation through upregulation of the cytoprotective enzyme heme oxygenase-1. In addition, H<sub>2</sub>O<sub>2</sub> exposure triggers HUVEC damage through the apoptosis process, as evidenced by increased cytochrome C release, Bax protein expression, and the elevated amount of activated caspase-3, however in HUVEC pretreated with peptides and their combination, the presence of those apoptotic stimuli was significantly decreased. Each peptide showed similar cytoprotective effect but no synergistic effect. Taken together, these peptides may be especially important in protecting against oxidative stress-mediated ED.Indyaswan Tegar SuryaningtyasChang-Bum AhnJae-Young JeMDPI AGarticlebioactive peptidecytoprotectiveoxidative stressendothelial dysfunctionblue musselBiology (General)QH301-705.5ENMarine Drugs, Vol 19, Iss 609, p 609 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	bioactive peptide cytoprotective oxidative stress endothelial dysfunction blue mussel Biology (General) QH301-705.5
spellingShingle	bioactive peptide cytoprotective oxidative stress endothelial dysfunction blue mussel Biology (General) QH301-705.5 Indyaswan Tegar Suryaningtyas Chang-Bum Ahn Jae-Young Je Cytoprotective Peptides from Blue Mussel Protein Hydrolysates: Identification and Mechanism Investigation in Human Umbilical Vein Endothelial Cells Injury
description	Cardiovascular disease represents a leading cause of mortality and is often characterized by the emergence of endothelial dysfunction (ED), a physiologic condition that takes place in the early progress of atherosclerosis. In this study, two cytoprotective peptides derived from blue mussel chymotrypsin hydrolysates with the sequence of EPTF and FTVN were purified and identified. Molecular mechanisms underlying the cytoprotective effects against oxidative stress which lead to human umbilical vein endothelial cells (HUVEC) injury were investigated. The results showed that pretreatment of EPTF, FTVN and their combination (1:1) in 0.1 mg/mL significantly reduced HUVEC death due to H<sub>2</sub>O<sub>2</sub> exposure. The cytoprotective mechanism of these peptides involves an improvement in the cellular antioxidant defense system, as indicated by the suppression of the intracellular ROS generation through upregulation of the cytoprotective enzyme heme oxygenase-1. In addition, H<sub>2</sub>O<sub>2</sub> exposure triggers HUVEC damage through the apoptosis process, as evidenced by increased cytochrome C release, Bax protein expression, and the elevated amount of activated caspase-3, however in HUVEC pretreated with peptides and their combination, the presence of those apoptotic stimuli was significantly decreased. Each peptide showed similar cytoprotective effect but no synergistic effect. Taken together, these peptides may be especially important in protecting against oxidative stress-mediated ED.
format	article
author	Indyaswan Tegar Suryaningtyas Chang-Bum Ahn Jae-Young Je
author_facet	Indyaswan Tegar Suryaningtyas Chang-Bum Ahn Jae-Young Je
author_sort	Indyaswan Tegar Suryaningtyas
title	Cytoprotective Peptides from Blue Mussel Protein Hydrolysates: Identification and Mechanism Investigation in Human Umbilical Vein Endothelial Cells Injury
title_short	Cytoprotective Peptides from Blue Mussel Protein Hydrolysates: Identification and Mechanism Investigation in Human Umbilical Vein Endothelial Cells Injury
title_full	Cytoprotective Peptides from Blue Mussel Protein Hydrolysates: Identification and Mechanism Investigation in Human Umbilical Vein Endothelial Cells Injury
title_fullStr	Cytoprotective Peptides from Blue Mussel Protein Hydrolysates: Identification and Mechanism Investigation in Human Umbilical Vein Endothelial Cells Injury
title_full_unstemmed	Cytoprotective Peptides from Blue Mussel Protein Hydrolysates: Identification and Mechanism Investigation in Human Umbilical Vein Endothelial Cells Injury
title_sort	cytoprotective peptides from blue mussel protein hydrolysates: identification and mechanism investigation in human umbilical vein endothelial cells injury
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/cf1175adf48c43a1b887c972c28b50bc
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Cytoprotective Peptides from Blue Mussel Protein Hydrolysates: Identification and Mechanism Investigation in Human Umbilical Vein Endothelial Cells Injury

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