Exploiting sorghum genetic diversity for enhanced aluminum tolerance: Allele mining based on the Alt SB locus

Abstract Root damage due to aluminum (Al) toxicity restricts crop production on acidic soils, which are extensive in the tropics. The sorghum root Al-activated citrate transporter, SbMATE, underlies the Al tolerance locus, Alt SB , and increases grain yield under Al toxicity. Here, Alt SB loci assoc...

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Autores principales:	Barbara Hufnagel, Claudia T. Guimaraes, Eric J. Craft, Jon E. Shaff, Robert E. Schaffert, Leon V. Kochian, Jurandir V. Magalhaes
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/cf15140f4cef42f3b2dff8f39945f6a3
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spelling	oai:doaj.org-article:cf15140f4cef42f3b2dff8f39945f6a32021-12-02T15:08:38ZExploiting sorghum genetic diversity for enhanced aluminum tolerance: Allele mining based on the Alt SB locus10.1038/s41598-018-27817-z2045-2322https://doaj.org/article/cf15140f4cef42f3b2dff8f39945f6a32018-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-27817-zhttps://doaj.org/toc/2045-2322Abstract Root damage due to aluminum (Al) toxicity restricts crop production on acidic soils, which are extensive in the tropics. The sorghum root Al-activated citrate transporter, SbMATE, underlies the Al tolerance locus, Alt SB , and increases grain yield under Al toxicity. Here, Alt SB loci associated with Al tolerance were converted into Amplification Refractory Mutation System (ARMS) markers, which are cost effective and easy to use. A DNA pooling strategy allowed us to identify accessions harboring rare favorable Alt SB alleles in three germplasm sets while greatly reducing genotyping needs. Population structure analysis revealed that favorable Alt SB alleles are predominantly found in subpopulations enriched with guinea sorghums, supporting a possible Western African origin of Alt SB . The efficiency of allele mining in recovering Al tolerance accessions was the highest in the largest and highly diverse germplasm set, with a 10-fold reduction in the number of accessions that would need to be phenotyped in the absence of marker information. Finally, Al tolerant accessions were found to rely on SbMATE to exclude Al3+ from sensitive sites in the root apex. This study emphasizes gene-specific markers as important tools for efficiently mining useful rare alleles in diverse germplasm, bridging genetic resource conservation efforts and pre-breeding for Al tolerance.Barbara HufnagelClaudia T. GuimaraesEric J. CraftJon E. ShaffRobert E. SchaffertLeon V. KochianJurandir V. MagalhaesNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-13 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Barbara Hufnagel Claudia T. Guimaraes Eric J. Craft Jon E. Shaff Robert E. Schaffert Leon V. Kochian Jurandir V. Magalhaes Exploiting sorghum genetic diversity for enhanced aluminum tolerance: Allele mining based on the Alt SB locus
description	Abstract Root damage due to aluminum (Al) toxicity restricts crop production on acidic soils, which are extensive in the tropics. The sorghum root Al-activated citrate transporter, SbMATE, underlies the Al tolerance locus, Alt SB , and increases grain yield under Al toxicity. Here, Alt SB loci associated with Al tolerance were converted into Amplification Refractory Mutation System (ARMS) markers, which are cost effective and easy to use. A DNA pooling strategy allowed us to identify accessions harboring rare favorable Alt SB alleles in three germplasm sets while greatly reducing genotyping needs. Population structure analysis revealed that favorable Alt SB alleles are predominantly found in subpopulations enriched with guinea sorghums, supporting a possible Western African origin of Alt SB . The efficiency of allele mining in recovering Al tolerance accessions was the highest in the largest and highly diverse germplasm set, with a 10-fold reduction in the number of accessions that would need to be phenotyped in the absence of marker information. Finally, Al tolerant accessions were found to rely on SbMATE to exclude Al3+ from sensitive sites in the root apex. This study emphasizes gene-specific markers as important tools for efficiently mining useful rare alleles in diverse germplasm, bridging genetic resource conservation efforts and pre-breeding for Al tolerance.
format	article
author	Barbara Hufnagel Claudia T. Guimaraes Eric J. Craft Jon E. Shaff Robert E. Schaffert Leon V. Kochian Jurandir V. Magalhaes
author_facet	Barbara Hufnagel Claudia T. Guimaraes Eric J. Craft Jon E. Shaff Robert E. Schaffert Leon V. Kochian Jurandir V. Magalhaes
author_sort	Barbara Hufnagel
title	Exploiting sorghum genetic diversity for enhanced aluminum tolerance: Allele mining based on the Alt SB locus
title_short	Exploiting sorghum genetic diversity for enhanced aluminum tolerance: Allele mining based on the Alt SB locus
title_full	Exploiting sorghum genetic diversity for enhanced aluminum tolerance: Allele mining based on the Alt SB locus
title_fullStr	Exploiting sorghum genetic diversity for enhanced aluminum tolerance: Allele mining based on the Alt SB locus
title_full_unstemmed	Exploiting sorghum genetic diversity for enhanced aluminum tolerance: Allele mining based on the Alt SB locus
title_sort	exploiting sorghum genetic diversity for enhanced aluminum tolerance: allele mining based on the alt sb locus
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/cf15140f4cef42f3b2dff8f39945f6a3
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Exploiting sorghum genetic diversity for enhanced aluminum tolerance: Allele mining based on the Alt SB locus

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