Oral Toxicity of <i>Pseudomonas protegens</i> against Muscoid Flies
The bioinsecticidal action of <i>Pseudomonas protegens</i> has so far been reported against some target insects, and the mode of action remains unclear. In this study, the pathogenicity potential of a recently isolated strain of this bacterial species against fly larvae of medical and ve...
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| Autores principales: | , |
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| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
MDPI AG
2021
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| Materias: | |
| Acceso en línea: | https://doaj.org/article/cf16a8a6ae3349e289102679960ec547 |
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| Sumario: | The bioinsecticidal action of <i>Pseudomonas protegens</i> has so far been reported against some target insects, and the mode of action remains unclear. In this study, the pathogenicity potential of a recently isolated strain of this bacterial species against fly larvae of medical and veterinary interest was determined. Preliminary experiments were conducted to determine the biocidal action by ingestion against <i>Musca domestica</i> and <i>Lucilia caesar</i> larvae, which highlighted a concentration-dependent effect, with LC<sub>50</sub> values of 3.6 and 2.5 × 10<sup>8</sup> CFU/mL, respectively. Bacterial septicaemia was observed in the body of insects assuming bacterial cells by ingestion. Such rapid bacterial reproduction in the hemolymph supports a toxin-mediated mechanism of action involving the intestinal barrier overcoming. In order to gain more information on the interaction with the host, the relative time-course expression of selected <i>P. protegens</i> genes associated with virulence and pathogenicity, was determined by qPCR at the gut level during the first infection stage. Among target genes, <i>chitinase D</i> was the most expressed, followed by <i>pesticin</i> and the fluorescent insecticidal toxin <i>fitD</i>. According to our observations and to the diversity of metabolites <i>P. protegens</i> produces, the pathogenic interaction this bacterium can establish with different targets appears to be complex and multifactorial. |
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