Oral Toxicity of <i>Pseudomonas protegens</i> against Muscoid Flies

The bioinsecticidal action of <i>Pseudomonas protegens</i> has so far been reported against some target insects, and the mode of action remains unclear. In this study, the pathogenicity potential of a recently isolated strain of this bacterial species against fly larvae of medical and ve...

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Autores principales:	Luca Ruiu, Maria Elena Mura
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	entomopathogens microbial biological control insecticidal genes virulence factors pest management Medicine R
Acceso en línea:	https://doaj.org/article/cf16a8a6ae3349e289102679960ec547
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spelling	oai:doaj.org-article:cf16a8a6ae3349e289102679960ec5472021-11-25T19:08:42ZOral Toxicity of <i>Pseudomonas protegens</i> against Muscoid Flies10.3390/toxins131107722072-6651https://doaj.org/article/cf16a8a6ae3349e289102679960ec5472021-11-01T00:00:00Zhttps://www.mdpi.com/2072-6651/13/11/772https://doaj.org/toc/2072-6651The bioinsecticidal action of <i>Pseudomonas protegens</i> has so far been reported against some target insects, and the mode of action remains unclear. In this study, the pathogenicity potential of a recently isolated strain of this bacterial species against fly larvae of medical and veterinary interest was determined. Preliminary experiments were conducted to determine the biocidal action by ingestion against <i>Musca domestica</i> and <i>Lucilia caesar</i> larvae, which highlighted a concentration-dependent effect, with LC<sub>50</sub> values of 3.6 and 2.5 × 10<sup>8</sup> CFU/mL, respectively. Bacterial septicaemia was observed in the body of insects assuming bacterial cells by ingestion. Such rapid bacterial reproduction in the hemolymph supports a toxin-mediated mechanism of action involving the intestinal barrier overcoming. In order to gain more information on the interaction with the host, the relative time-course expression of selected <i>P. protegens</i> genes associated with virulence and pathogenicity, was determined by qPCR at the gut level during the first infection stage. Among target genes, <i>chitinase D</i> was the most expressed, followed by <i>pesticin</i> and the fluorescent insecticidal toxin <i>fitD</i>. According to our observations and to the diversity of metabolites <i>P. protegens</i> produces, the pathogenic interaction this bacterium can establish with different targets appears to be complex and multifactorial.Luca RuiuMaria Elena MuraMDPI AGarticleentomopathogensmicrobialbiological controlinsecticidal genesvirulence factorspest managementMedicineRENToxins, Vol 13, Iss 772, p 772 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	entomopathogens microbial biological control insecticidal genes virulence factors pest management Medicine R
spellingShingle	entomopathogens microbial biological control insecticidal genes virulence factors pest management Medicine R Luca Ruiu Maria Elena Mura Oral Toxicity of <i>Pseudomonas protegens</i> against Muscoid Flies
description	The bioinsecticidal action of <i>Pseudomonas protegens</i> has so far been reported against some target insects, and the mode of action remains unclear. In this study, the pathogenicity potential of a recently isolated strain of this bacterial species against fly larvae of medical and veterinary interest was determined. Preliminary experiments were conducted to determine the biocidal action by ingestion against <i>Musca domestica</i> and <i>Lucilia caesar</i> larvae, which highlighted a concentration-dependent effect, with LC<sub>50</sub> values of 3.6 and 2.5 × 10<sup>8</sup> CFU/mL, respectively. Bacterial septicaemia was observed in the body of insects assuming bacterial cells by ingestion. Such rapid bacterial reproduction in the hemolymph supports a toxin-mediated mechanism of action involving the intestinal barrier overcoming. In order to gain more information on the interaction with the host, the relative time-course expression of selected <i>P. protegens</i> genes associated with virulence and pathogenicity, was determined by qPCR at the gut level during the first infection stage. Among target genes, <i>chitinase D</i> was the most expressed, followed by <i>pesticin</i> and the fluorescent insecticidal toxin <i>fitD</i>. According to our observations and to the diversity of metabolites <i>P. protegens</i> produces, the pathogenic interaction this bacterium can establish with different targets appears to be complex and multifactorial.
format	article
author	Luca Ruiu Maria Elena Mura
author_facet	Luca Ruiu Maria Elena Mura
author_sort	Luca Ruiu
title	Oral Toxicity of <i>Pseudomonas protegens</i> against Muscoid Flies
title_short	Oral Toxicity of <i>Pseudomonas protegens</i> against Muscoid Flies
title_full	Oral Toxicity of <i>Pseudomonas protegens</i> against Muscoid Flies
title_fullStr	Oral Toxicity of <i>Pseudomonas protegens</i> against Muscoid Flies
title_full_unstemmed	Oral Toxicity of <i>Pseudomonas protegens</i> against Muscoid Flies
title_sort	oral toxicity of <i>pseudomonas protegens</i> against muscoid flies
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/cf16a8a6ae3349e289102679960ec547
work_keys_str_mv	AT lucaruiu oraltoxicityofipseudomonasprotegensiagainstmuscoidflies AT mariaelenamura oraltoxicityofipseudomonasprotegensiagainstmuscoidflies
_version_	1718410244726456320

Oral Toxicity of <i>Pseudomonas protegens</i> against Muscoid Flies

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