Linking splicing to Pol II transcription stabilizes pre-mRNAs and influences splicing patterns.

RNA processing is carried out in close proximity to the site of transcription, suggesting a regulatory link between transcription and pre-mRNA splicing. Using an in vitro transcription/splicing assay, we demonstrate that an association of RNA polymerase II (Pol II) transcription and pre-mRNA splicin...

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Autores principales:	Martin J Hicks, Chin-Rang Yang, Matthew V Kotlajich, Klemens J Hertel
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2006
Materias:	Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/cf3042a3e34a4732ba67adaac8d51c96
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spelling	oai:doaj.org-article:cf3042a3e34a4732ba67adaac8d51c962021-11-25T05:33:10ZLinking splicing to Pol II transcription stabilizes pre-mRNAs and influences splicing patterns.1544-91731545-788510.1371/journal.pbio.0040147https://doaj.org/article/cf3042a3e34a4732ba67adaac8d51c962006-06-01T00:00:00Zhttps://doi.org/10.1371/journal.pbio.0040147https://doaj.org/toc/1544-9173https://doaj.org/toc/1545-7885RNA processing is carried out in close proximity to the site of transcription, suggesting a regulatory link between transcription and pre-mRNA splicing. Using an in vitro transcription/splicing assay, we demonstrate that an association of RNA polymerase II (Pol II) transcription and pre-mRNA splicing is required for efficient gene expression. Pol II-synthesized RNAs containing functional splice sites are protected from nuclear degradation, presumably because the local concentration of the splicing machinery is sufficiently high to ensure its association over interactions with nucleases. Furthermore, the process of transcription influences alternative splicing of newly synthesized pre-mRNAs. Because other RNA polymerases do not provide similar protection from nucleases, and their RNA products display altered splicing patterns, the link between transcription and RNA processing is RNA Pol II-specific. We propose that the connection between transcription by Pol II and pre-mRNA splicing guarantees an extended half-life and proper processing of nascent pre-mRNAs.Martin J HicksChin-Rang YangMatthew V KotlajichKlemens J HertelPublic Library of Science (PLoS)articleBiology (General)QH301-705.5ENPLoS Biology, Vol 4, Iss 6, p e147 (2006)
institution	DOAJ
collection	DOAJ
language	EN
topic	Biology (General) QH301-705.5
spellingShingle	Biology (General) QH301-705.5 Martin J Hicks Chin-Rang Yang Matthew V Kotlajich Klemens J Hertel Linking splicing to Pol II transcription stabilizes pre-mRNAs and influences splicing patterns.
description	RNA processing is carried out in close proximity to the site of transcription, suggesting a regulatory link between transcription and pre-mRNA splicing. Using an in vitro transcription/splicing assay, we demonstrate that an association of RNA polymerase II (Pol II) transcription and pre-mRNA splicing is required for efficient gene expression. Pol II-synthesized RNAs containing functional splice sites are protected from nuclear degradation, presumably because the local concentration of the splicing machinery is sufficiently high to ensure its association over interactions with nucleases. Furthermore, the process of transcription influences alternative splicing of newly synthesized pre-mRNAs. Because other RNA polymerases do not provide similar protection from nucleases, and their RNA products display altered splicing patterns, the link between transcription and RNA processing is RNA Pol II-specific. We propose that the connection between transcription by Pol II and pre-mRNA splicing guarantees an extended half-life and proper processing of nascent pre-mRNAs.
format	article
author	Martin J Hicks Chin-Rang Yang Matthew V Kotlajich Klemens J Hertel
author_facet	Martin J Hicks Chin-Rang Yang Matthew V Kotlajich Klemens J Hertel
author_sort	Martin J Hicks
title	Linking splicing to Pol II transcription stabilizes pre-mRNAs and influences splicing patterns.
title_short	Linking splicing to Pol II transcription stabilizes pre-mRNAs and influences splicing patterns.
title_full	Linking splicing to Pol II transcription stabilizes pre-mRNAs and influences splicing patterns.
title_fullStr	Linking splicing to Pol II transcription stabilizes pre-mRNAs and influences splicing patterns.
title_full_unstemmed	Linking splicing to Pol II transcription stabilizes pre-mRNAs and influences splicing patterns.
title_sort	linking splicing to pol ii transcription stabilizes pre-mrnas and influences splicing patterns.
publisher	Public Library of Science (PLoS)
publishDate	2006
url	https://doaj.org/article/cf3042a3e34a4732ba67adaac8d51c96
work_keys_str_mv	AT martinjhicks linkingsplicingtopoliitranscriptionstabilizespremrnasandinfluencessplicingpatterns AT chinrangyang linkingsplicingtopoliitranscriptionstabilizespremrnasandinfluencessplicingpatterns AT matthewvkotlajich linkingsplicingtopoliitranscriptionstabilizespremrnasandinfluencessplicingpatterns AT klemensjhertel linkingsplicingtopoliitranscriptionstabilizespremrnasandinfluencessplicingpatterns
_version_	1718414666452959232

Linking splicing to Pol II transcription stabilizes pre-mRNAs and influences splicing patterns.

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