Phenotypic variation and genetic alteration of Spathoglottis plicata resulted from in vitro cultured seed irradiated with X-Ray

Aloysius S, Purwantoro A, Dewi K, Semiarti E.. 2018. Phenotypic variation and genetic alteration of Spathoglottis plicata resulted from in vitro cultured seed irradiated with X-Ray. Biodiversitas 19: 1642-1648. A terrestrial orchid species among genus Spathoglottis as widely cultivated is S. plicata...

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Autores principales:	SUYITNO ALOYSIUS, AZIZ PURWANTORO, KUMALA DEWI, ENDANG SEMIARTI
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2018
Materias:	genetic phenotypic poh1 homologous gene x-ray Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/cf35140ccc1c4b54bc856cdd12a703c0
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spelling	oai:doaj.org-article:cf35140ccc1c4b54bc856cdd12a703c02021-11-16T14:04:05ZPhenotypic variation and genetic alteration of Spathoglottis plicata resulted from in vitro cultured seed irradiated with X-Ray1412-033X2085-472210.13057/biodiv/d190507https://doaj.org/article/cf35140ccc1c4b54bc856cdd12a703c02018-09-01T00:00:00Zhttps://smujo.id/biodiv/article/view/2923https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Aloysius S, Purwantoro A, Dewi K, Semiarti E.. 2018. Phenotypic variation and genetic alteration of Spathoglottis plicata resulted from in vitro cultured seed irradiated with X-Ray. Biodiversitas 19: 1642-1648. A terrestrial orchid species among genus Spathoglottis as widely cultivated is S. plicata. Variability development of the species through mutation induction has been carried out, but its morphological variations and genetic changes have not been investigated. The purpose of this study is to identify the phenotypic variation and genetic alteration of S. plicata resulted from in vitro cultured seed irradiated with X-ray. Radiation was given at the doses of 0; 6; 12; 18 and 24 rad. The samples were surviving plants resulted from irradiated seeds. Phenotypic variations observed were the number, length and width of the leaf, number of tiller, and flower characteristics. Genetic alteration was detected from DNA homologous POH1, a key gene determining of shoot morphogenesis. Results show that there are variations of leaf color, length and width of the leaf, and the number of the tiller. Plants start to flower at the age of 30 months. The plants flowering reach 64.7% (WT), 50.0% (6 rad), 33.3% (12 rad), 33.3% (18 rad), and 40% (24 rad). Flower color is ranged from white, white slightly purple, purplish white, light purple, reddish purple and purple, found both in mutants and wild-type groups. The alignment result of POH1 homologous DNA obtained from PCR cDNA shows the nucleotide differences at some points between mutants and wild-type that indicate the occurrence of DNA alteration. X-ray induces the changes of POH1 homologous DNA, but it has no obvious relationship to the flower variation.SUYITNO ALOYSIUSAZIZ PURWANTOROKUMALA DEWIENDANG SEMIARTIMBI & UNS Soloarticlegeneticphenotypicpoh1 homologous genex-rayBiology (General)QH301-705.5ENBiodiversitas, Vol 19, Iss 5, Pp 1642-1648 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	genetic phenotypic poh1 homologous gene x-ray Biology (General) QH301-705.5
spellingShingle	genetic phenotypic poh1 homologous gene x-ray Biology (General) QH301-705.5 SUYITNO ALOYSIUS AZIZ PURWANTORO KUMALA DEWI ENDANG SEMIARTI Phenotypic variation and genetic alteration of Spathoglottis plicata resulted from in vitro cultured seed irradiated with X-Ray
description	Aloysius S, Purwantoro A, Dewi K, Semiarti E.. 2018. Phenotypic variation and genetic alteration of Spathoglottis plicata resulted from in vitro cultured seed irradiated with X-Ray. Biodiversitas 19: 1642-1648. A terrestrial orchid species among genus Spathoglottis as widely cultivated is S. plicata. Variability development of the species through mutation induction has been carried out, but its morphological variations and genetic changes have not been investigated. The purpose of this study is to identify the phenotypic variation and genetic alteration of S. plicata resulted from in vitro cultured seed irradiated with X-ray. Radiation was given at the doses of 0; 6; 12; 18 and 24 rad. The samples were surviving plants resulted from irradiated seeds. Phenotypic variations observed were the number, length and width of the leaf, number of tiller, and flower characteristics. Genetic alteration was detected from DNA homologous POH1, a key gene determining of shoot morphogenesis. Results show that there are variations of leaf color, length and width of the leaf, and the number of the tiller. Plants start to flower at the age of 30 months. The plants flowering reach 64.7% (WT), 50.0% (6 rad), 33.3% (12 rad), 33.3% (18 rad), and 40% (24 rad). Flower color is ranged from white, white slightly purple, purplish white, light purple, reddish purple and purple, found both in mutants and wild-type groups. The alignment result of POH1 homologous DNA obtained from PCR cDNA shows the nucleotide differences at some points between mutants and wild-type that indicate the occurrence of DNA alteration. X-ray induces the changes of POH1 homologous DNA, but it has no obvious relationship to the flower variation.
format	article
author	SUYITNO ALOYSIUS AZIZ PURWANTORO KUMALA DEWI ENDANG SEMIARTI
author_facet	SUYITNO ALOYSIUS AZIZ PURWANTORO KUMALA DEWI ENDANG SEMIARTI
author_sort	SUYITNO ALOYSIUS
title	Phenotypic variation and genetic alteration of Spathoglottis plicata resulted from in vitro cultured seed irradiated with X-Ray
title_short	Phenotypic variation and genetic alteration of Spathoglottis plicata resulted from in vitro cultured seed irradiated with X-Ray
title_full	Phenotypic variation and genetic alteration of Spathoglottis plicata resulted from in vitro cultured seed irradiated with X-Ray
title_fullStr	Phenotypic variation and genetic alteration of Spathoglottis plicata resulted from in vitro cultured seed irradiated with X-Ray
title_full_unstemmed	Phenotypic variation and genetic alteration of Spathoglottis plicata resulted from in vitro cultured seed irradiated with X-Ray
title_sort	phenotypic variation and genetic alteration of spathoglottis plicata resulted from in vitro cultured seed irradiated with x-ray
publisher	MBI & UNS Solo
publishDate	2018
url	https://doaj.org/article/cf35140ccc1c4b54bc856cdd12a703c0
work_keys_str_mv	AT suyitnoaloysius phenotypicvariationandgeneticalterationofspathoglottisplicataresultedfrominvitroculturedseedirradiatedwithxray AT azizpurwantoro phenotypicvariationandgeneticalterationofspathoglottisplicataresultedfrominvitroculturedseedirradiatedwithxray AT kumaladewi phenotypicvariationandgeneticalterationofspathoglottisplicataresultedfrominvitroculturedseedirradiatedwithxray AT endangsemiarti phenotypicvariationandgeneticalterationofspathoglottisplicataresultedfrominvitroculturedseedirradiatedwithxray
_version_	1718426450534596608

Phenotypic variation and genetic alteration of Spathoglottis plicata resulted from in vitro cultured seed irradiated with X-Ray

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