Ultramarine, a chromoprotein acceptor for Förster resonance energy transfer.

We have engineered a monomeric blue non-fluorescent chromoprotein called Ultramarine (fluorescence quantum yield, 0.001; ε(585 nm), 64,000 M(-1) x cm(-1)) for use as a Förster resonance energy transfer acceptor for a number of different donor fluorescent proteins. We show its use for monitoring acti...

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Autores principales:	Anne Pettikiriarachchi, Lan Gong, Matthew A Perugini, Rodney J Devenish, Mark Prescott
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/cf35399bb2114e3eb6ab6d430e2b6776
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spelling	oai:doaj.org-article:cf35399bb2114e3eb6ab6d430e2b67762021-11-18T07:12:19ZUltramarine, a chromoprotein acceptor for Förster resonance energy transfer.1932-620310.1371/journal.pone.0041028https://doaj.org/article/cf35399bb2114e3eb6ab6d430e2b67762012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22815901/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203We have engineered a monomeric blue non-fluorescent chromoprotein called Ultramarine (fluorescence quantum yield, 0.001; ε(585 nm), 64,000 M(-1) x cm(-1)) for use as a Förster resonance energy transfer acceptor for a number of different donor fluorescent proteins. We show its use for monitoring activation of caspase 3 in live cells using fluorescence lifetime imaging. Ultramarine has the potential to increase the number of cellular parameters that can be imaged simultaneously.Anne PettikiriarachchiLan GongMatthew A PeruginiRodney J DevenishMark PrescottPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 7, p e41028 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Anne Pettikiriarachchi Lan Gong Matthew A Perugini Rodney J Devenish Mark Prescott Ultramarine, a chromoprotein acceptor for Förster resonance energy transfer.
description	We have engineered a monomeric blue non-fluorescent chromoprotein called Ultramarine (fluorescence quantum yield, 0.001; ε(585 nm), 64,000 M(-1) x cm(-1)) for use as a Förster resonance energy transfer acceptor for a number of different donor fluorescent proteins. We show its use for monitoring activation of caspase 3 in live cells using fluorescence lifetime imaging. Ultramarine has the potential to increase the number of cellular parameters that can be imaged simultaneously.
format	article
author	Anne Pettikiriarachchi Lan Gong Matthew A Perugini Rodney J Devenish Mark Prescott
author_facet	Anne Pettikiriarachchi Lan Gong Matthew A Perugini Rodney J Devenish Mark Prescott
author_sort	Anne Pettikiriarachchi
title	Ultramarine, a chromoprotein acceptor for Förster resonance energy transfer.
title_short	Ultramarine, a chromoprotein acceptor for Förster resonance energy transfer.
title_full	Ultramarine, a chromoprotein acceptor for Förster resonance energy transfer.
title_fullStr	Ultramarine, a chromoprotein acceptor for Förster resonance energy transfer.
title_full_unstemmed	Ultramarine, a chromoprotein acceptor for Förster resonance energy transfer.
title_sort	ultramarine, a chromoprotein acceptor for förster resonance energy transfer.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/cf35399bb2114e3eb6ab6d430e2b6776
work_keys_str_mv	AT annepettikiriarachchi ultramarineachromoproteinacceptorforforsterresonanceenergytransfer AT langong ultramarineachromoproteinacceptorforforsterresonanceenergytransfer AT matthewaperugini ultramarineachromoproteinacceptorforforsterresonanceenergytransfer AT rodneyjdevenish ultramarineachromoproteinacceptorforforsterresonanceenergytransfer AT markprescott ultramarineachromoproteinacceptorforforsterresonanceenergytransfer
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Ultramarine, a chromoprotein acceptor for Förster resonance energy transfer.

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