Quorum Sensing Extracellular Death Peptides Enhance the Endoribonucleolytic Activities of <italic toggle="yes">Mycobacterium tuberculosis</italic> MazF Toxins

ABSTRACT mazEF is a toxin-antitoxin module located on chromosomes of most bacteria. MazF toxins are endoribonucleases antagonized by MazE antitoxins. Previously, we characterized several quorum sensing peptides called "extracellular death factors" (EDFs). When secreted from bacterial cultu...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Akanksha Nigam, Sathish Kumar, Hanna Engelberg-Kulka
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2018
Materias:	extracellular death peptides Mycobacterium tuberculosis quorum sensing Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/cf46194c78fe48788e488bdc82d029a9
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:cf46194c78fe48788e488bdc82d029a9
record_format	dspace
spelling	oai:doaj.org-article:cf46194c78fe48788e488bdc82d029a92021-11-15T16:00:26ZQuorum Sensing Extracellular Death Peptides Enhance the Endoribonucleolytic Activities of <italic toggle="yes">Mycobacterium tuberculosis</italic> MazF Toxins10.1128/mBio.00685-182150-7511https://doaj.org/article/cf46194c78fe48788e488bdc82d029a92018-07-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mBio.00685-18https://doaj.org/toc/2150-7511ABSTRACT mazEF is a toxin-antitoxin module located on chromosomes of most bacteria. MazF toxins are endoribonucleases antagonized by MazE antitoxins. Previously, we characterized several quorum sensing peptides called "extracellular death factors" (EDFs). When secreted from bacterial cultures, EDFs induce interspecies cell death. EDFs also enhance the endoribonucleolytic activity of Escherichia coli MazF. Mycobacterium tuberculosis carries several mazEF modules. Among them, the endoribonucleolytic activities of MazF proteins mt-1, mt-3, and mt-6 were identified. MazF-mt6 and MazF-mt-3 cleave M. tuberculosis rRNAs. Here we report the in vitro effects of EDFs on the endoribonucleolytic activities of M. tuberculosis MazFs. Escherichia coli EDF (EcEDF) and the three Pseudomonas aeruginosa EDFs (PaEDFs) individually enhance the endoribonucleolytic activities of MazF-mt6 and MazF-mt3 and overcome the inhibitory effect of MazE-mt3 or MazE-mt6 on the endoribonucleolytic activities of the respective toxins. We propose that these EDFs can serve as a basis for a novel class of antibiotics against M. tuberculosis. IMPORTANCE Mycobacterium tuberculosis is one of the leading causes of death from infectious disease. M. tuberculosis is highly drug resistant, and drug delivery to the infected site is very difficult. In previous studies, we showed that extracellular death factors (EDFs) can work as quorum sensing molecules which participate in interspecies bacterial cell death. In this study, we demonstrated the role of different EDFs in the endoribonucleolytic activities of M. tuberculosis MazFs. Escherichia coli EDF (EcEDF) and the three Pseudomonas aeruginosa EDFs (PaEDFs) individually enhance the endoribonucleolytic activities of MazF-mt6 and MazF-mt3. The current report provides a basis for the use of the EDF peptides EcEDF and PaEDF as novel antibiotics against M. tuberculosis.Akanksha NigamSathish KumarHanna Engelberg-KulkaAmerican Society for Microbiologyarticleextracellular death peptidesMycobacterium tuberculosisquorum sensingMicrobiologyQR1-502ENmBio, Vol 9, Iss 3 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	extracellular death peptides Mycobacterium tuberculosis quorum sensing Microbiology QR1-502
spellingShingle	extracellular death peptides Mycobacterium tuberculosis quorum sensing Microbiology QR1-502 Akanksha Nigam Sathish Kumar Hanna Engelberg-Kulka Quorum Sensing Extracellular Death Peptides Enhance the Endoribonucleolytic Activities of <italic toggle="yes">Mycobacterium tuberculosis</italic> MazF Toxins
description	ABSTRACT mazEF is a toxin-antitoxin module located on chromosomes of most bacteria. MazF toxins are endoribonucleases antagonized by MazE antitoxins. Previously, we characterized several quorum sensing peptides called "extracellular death factors" (EDFs). When secreted from bacterial cultures, EDFs induce interspecies cell death. EDFs also enhance the endoribonucleolytic activity of Escherichia coli MazF. Mycobacterium tuberculosis carries several mazEF modules. Among them, the endoribonucleolytic activities of MazF proteins mt-1, mt-3, and mt-6 were identified. MazF-mt6 and MazF-mt-3 cleave M. tuberculosis rRNAs. Here we report the in vitro effects of EDFs on the endoribonucleolytic activities of M. tuberculosis MazFs. Escherichia coli EDF (EcEDF) and the three Pseudomonas aeruginosa EDFs (PaEDFs) individually enhance the endoribonucleolytic activities of MazF-mt6 and MazF-mt3 and overcome the inhibitory effect of MazE-mt3 or MazE-mt6 on the endoribonucleolytic activities of the respective toxins. We propose that these EDFs can serve as a basis for a novel class of antibiotics against M. tuberculosis. IMPORTANCE Mycobacterium tuberculosis is one of the leading causes of death from infectious disease. M. tuberculosis is highly drug resistant, and drug delivery to the infected site is very difficult. In previous studies, we showed that extracellular death factors (EDFs) can work as quorum sensing molecules which participate in interspecies bacterial cell death. In this study, we demonstrated the role of different EDFs in the endoribonucleolytic activities of M. tuberculosis MazFs. Escherichia coli EDF (EcEDF) and the three Pseudomonas aeruginosa EDFs (PaEDFs) individually enhance the endoribonucleolytic activities of MazF-mt6 and MazF-mt3. The current report provides a basis for the use of the EDF peptides EcEDF and PaEDF as novel antibiotics against M. tuberculosis.
format	article
author	Akanksha Nigam Sathish Kumar Hanna Engelberg-Kulka
author_facet	Akanksha Nigam Sathish Kumar Hanna Engelberg-Kulka
author_sort	Akanksha Nigam
title	Quorum Sensing Extracellular Death Peptides Enhance the Endoribonucleolytic Activities of <italic toggle="yes">Mycobacterium tuberculosis</italic> MazF Toxins
title_short	Quorum Sensing Extracellular Death Peptides Enhance the Endoribonucleolytic Activities of <italic toggle="yes">Mycobacterium tuberculosis</italic> MazF Toxins
title_full	Quorum Sensing Extracellular Death Peptides Enhance the Endoribonucleolytic Activities of <italic toggle="yes">Mycobacterium tuberculosis</italic> MazF Toxins
title_fullStr	Quorum Sensing Extracellular Death Peptides Enhance the Endoribonucleolytic Activities of <italic toggle="yes">Mycobacterium tuberculosis</italic> MazF Toxins
title_full_unstemmed	Quorum Sensing Extracellular Death Peptides Enhance the Endoribonucleolytic Activities of <italic toggle="yes">Mycobacterium tuberculosis</italic> MazF Toxins
title_sort	quorum sensing extracellular death peptides enhance the endoribonucleolytic activities of <italic toggle="yes">mycobacterium tuberculosis</italic> mazf toxins
publisher	American Society for Microbiology
publishDate	2018
url	https://doaj.org/article/cf46194c78fe48788e488bdc82d029a9
work_keys_str_mv	AT akankshanigam quorumsensingextracellulardeathpeptidesenhancetheendoribonucleolyticactivitiesofitalictoggleyesmycobacteriumtuberculosisitalicmazftoxins AT sathishkumar quorumsensingextracellulardeathpeptidesenhancetheendoribonucleolyticactivitiesofitalictoggleyesmycobacteriumtuberculosisitalicmazftoxins AT hannaengelbergkulka quorumsensingextracellulardeathpeptidesenhancetheendoribonucleolyticactivitiesofitalictoggleyesmycobacteriumtuberculosisitalicmazftoxins
_version_	1718426997089107968

Quorum Sensing Extracellular Death Peptides Enhance the Endoribonucleolytic Activities of <italic toggle="yes">Mycobacterium tuberculosis</italic> MazF Toxins

Ejemplares similares