Formulation of a reactive oxygen producing calcium sulphate cement as an anti-bacterial hard tissue scaffold

Abstract Prophylactic antibiotic bone cements are extensively used in orthopaedics. However, the development of antimicrobial resistance to antibiotics, demonstrates a need to find alternative treatments. Herein, an antimicrobial honey (SurgihoneyRO-SHRO) has been successfully incorporated into a ca...

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Autores principales:	Thomas J. Hall, Erik A. B. Hughes, Hamzah Sajjad, Sarah A. Kuehne, Melissa M. Grant, Liam M. Grover, Sophie C. Cox
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/cf54e03d6bbc49839fb7c589e48071f7
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spelling	oai:doaj.org-article:cf54e03d6bbc49839fb7c589e48071f72021-12-02T11:35:58ZFormulation of a reactive oxygen producing calcium sulphate cement as an anti-bacterial hard tissue scaffold10.1038/s41598-021-84060-92045-2322https://doaj.org/article/cf54e03d6bbc49839fb7c589e48071f72021-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-84060-9https://doaj.org/toc/2045-2322Abstract Prophylactic antibiotic bone cements are extensively used in orthopaedics. However, the development of antimicrobial resistance to antibiotics, demonstrates a need to find alternative treatments. Herein, an antimicrobial honey (SurgihoneyRO-SHRO) has been successfully incorporated into a calcium sulphate (CS) based cement to produce a hard tissue scaffold with the ability to inhibit bacterial growth. Antimicrobial properties elicited from SHRO are predominantly owed to the water-initiated production of reactive oxygen species (ROS). As an alternative to initially loading CS cement with SHRO, in order to prevent premature activation, SHRO was added into the already developing cement matrix, locking available water into the CS crystal structure before SHRO addition. Promisingly, this methodology produced > 2.5 times (715.0 ± 147.3 μM/mL/g) more ROS over 24 h and exhibited a compressive strength (32.2 ± 5.8 MPa) comparable to trabecular bone after 3 weeks of immersion. In-vitro the SHRO loaded CS scaffolds were shown to inhibit growth of clinically relevant organisms, Staphylococcus aureus and Pseudomonas aeruginosa, with comparable potency to equivalent doses of gentamicin. Encouragingly, formulations did not inhibit wound healing or induce an inflammatory response from osteoblasts. Overall this study highlights the prophylactic potential of CS-SHRO cements as an alternative to traditional antibiotics.Thomas J. HallErik A. B. HughesHamzah SajjadSarah A. KuehneMelissa M. GrantLiam M. GroverSophie C. CoxNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Thomas J. Hall Erik A. B. Hughes Hamzah Sajjad Sarah A. Kuehne Melissa M. Grant Liam M. Grover Sophie C. Cox Formulation of a reactive oxygen producing calcium sulphate cement as an anti-bacterial hard tissue scaffold
description	Abstract Prophylactic antibiotic bone cements are extensively used in orthopaedics. However, the development of antimicrobial resistance to antibiotics, demonstrates a need to find alternative treatments. Herein, an antimicrobial honey (SurgihoneyRO-SHRO) has been successfully incorporated into a calcium sulphate (CS) based cement to produce a hard tissue scaffold with the ability to inhibit bacterial growth. Antimicrobial properties elicited from SHRO are predominantly owed to the water-initiated production of reactive oxygen species (ROS). As an alternative to initially loading CS cement with SHRO, in order to prevent premature activation, SHRO was added into the already developing cement matrix, locking available water into the CS crystal structure before SHRO addition. Promisingly, this methodology produced > 2.5 times (715.0 ± 147.3 μM/mL/g) more ROS over 24 h and exhibited a compressive strength (32.2 ± 5.8 MPa) comparable to trabecular bone after 3 weeks of immersion. In-vitro the SHRO loaded CS scaffolds were shown to inhibit growth of clinically relevant organisms, Staphylococcus aureus and Pseudomonas aeruginosa, with comparable potency to equivalent doses of gentamicin. Encouragingly, formulations did not inhibit wound healing or induce an inflammatory response from osteoblasts. Overall this study highlights the prophylactic potential of CS-SHRO cements as an alternative to traditional antibiotics.
format	article
author	Thomas J. Hall Erik A. B. Hughes Hamzah Sajjad Sarah A. Kuehne Melissa M. Grant Liam M. Grover Sophie C. Cox
author_facet	Thomas J. Hall Erik A. B. Hughes Hamzah Sajjad Sarah A. Kuehne Melissa M. Grant Liam M. Grover Sophie C. Cox
author_sort	Thomas J. Hall
title	Formulation of a reactive oxygen producing calcium sulphate cement as an anti-bacterial hard tissue scaffold
title_short	Formulation of a reactive oxygen producing calcium sulphate cement as an anti-bacterial hard tissue scaffold
title_full	Formulation of a reactive oxygen producing calcium sulphate cement as an anti-bacterial hard tissue scaffold
title_fullStr	Formulation of a reactive oxygen producing calcium sulphate cement as an anti-bacterial hard tissue scaffold
title_full_unstemmed	Formulation of a reactive oxygen producing calcium sulphate cement as an anti-bacterial hard tissue scaffold
title_sort	formulation of a reactive oxygen producing calcium sulphate cement as an anti-bacterial hard tissue scaffold
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/cf54e03d6bbc49839fb7c589e48071f7
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Formulation of a reactive oxygen producing calcium sulphate cement as an anti-bacterial hard tissue scaffold

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