Integrated Transcriptomic and Proteomic Analysis in the Roadmap of the Xylem Development Stage in Populus tomentosa
Xylem development plays an important role in the wood formation of plants. In this study, we found that xylem development was a rapid thickening process characterized by initially rapid increases in the number of tracheary elements and fiber cells and the thickness of the secondary walls that later...
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Frontiers Media S.A.
2021
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oai:doaj.org-article:cf54e42e14d34b86915d8a946962b0692021-11-04T08:32:33ZIntegrated Transcriptomic and Proteomic Analysis in the Roadmap of the Xylem Development Stage in Populus tomentosa1664-462X10.3389/fpls.2021.724559https://doaj.org/article/cf54e42e14d34b86915d8a946962b0692021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fpls.2021.724559/fullhttps://doaj.org/toc/1664-462XXylem development plays an important role in the wood formation of plants. In this study, we found that xylem development was a rapid thickening process characterized by initially rapid increases in the number of tracheary elements and fiber cells and the thickness of the secondary walls that later plateaued. Transcriptome analysis showed that the xylan and lignin biosynthetic pathways, which are involved in the early rapid thickening of the xylem, were mainly upregulated in the second month. The expression of a total of 124 transcription factors (TFs), including 28 NAC TFs and 31 MYB TFs, peaked in 2- and 3-month-old plants compared with 1-month-old plants. Based on previous studies and the key cis-acting elements secondary wall NAC-binding elements, secondary wall MYB-responsive elements, W-box and TGTG[T/G/C], 10 TFs related to xylem development, 50 TFs with unknown function, 98 cell wall biosynthetic genes, and 47 programmed cell death (PCD) genes were used to construct a four-layer transcriptional regulatory network (TRN) with poplar NAC domain TFs to characterize the transcriptional regulation of cell wall biosynthesis and PCD in Populus tomentosa. The proteome revealed that post-transcriptional modification may be widely involved in lignification development. Overall, our results revealed that xylem development is a rapid thickening process in P. tomentosa, and expression patterns varied temporally from cell division to cell death.Chong ZhangChong ZhangJiaxue ZhangJiaxue ZhangYadi LiuYadi LiuXiatong LiuXiatong LiuXiaorui GuoXiaorui GuoHui LiHui LiDi LiuDi LiuHai LuHai LuFrontiers Media S.A.articlexylem developmenttranscriptomeproteometranscriptional regulationPopulus tomentosaPlant cultureSB1-1110ENFrontiers in Plant Science, Vol 12 (2021) |
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| topic |
xylem development transcriptome proteome transcriptional regulation Populus tomentosa Plant culture SB1-1110 |
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xylem development transcriptome proteome transcriptional regulation Populus tomentosa Plant culture SB1-1110 Chong Zhang Chong Zhang Jiaxue Zhang Jiaxue Zhang Yadi Liu Yadi Liu Xiatong Liu Xiatong Liu Xiaorui Guo Xiaorui Guo Hui Li Hui Li Di Liu Di Liu Hai Lu Hai Lu Integrated Transcriptomic and Proteomic Analysis in the Roadmap of the Xylem Development Stage in Populus tomentosa |
| description |
Xylem development plays an important role in the wood formation of plants. In this study, we found that xylem development was a rapid thickening process characterized by initially rapid increases in the number of tracheary elements and fiber cells and the thickness of the secondary walls that later plateaued. Transcriptome analysis showed that the xylan and lignin biosynthetic pathways, which are involved in the early rapid thickening of the xylem, were mainly upregulated in the second month. The expression of a total of 124 transcription factors (TFs), including 28 NAC TFs and 31 MYB TFs, peaked in 2- and 3-month-old plants compared with 1-month-old plants. Based on previous studies and the key cis-acting elements secondary wall NAC-binding elements, secondary wall MYB-responsive elements, W-box and TGTG[T/G/C], 10 TFs related to xylem development, 50 TFs with unknown function, 98 cell wall biosynthetic genes, and 47 programmed cell death (PCD) genes were used to construct a four-layer transcriptional regulatory network (TRN) with poplar NAC domain TFs to characterize the transcriptional regulation of cell wall biosynthesis and PCD in Populus tomentosa. The proteome revealed that post-transcriptional modification may be widely involved in lignification development. Overall, our results revealed that xylem development is a rapid thickening process in P. tomentosa, and expression patterns varied temporally from cell division to cell death. |
| format |
article |
| author |
Chong Zhang Chong Zhang Jiaxue Zhang Jiaxue Zhang Yadi Liu Yadi Liu Xiatong Liu Xiatong Liu Xiaorui Guo Xiaorui Guo Hui Li Hui Li Di Liu Di Liu Hai Lu Hai Lu |
| author_facet |
Chong Zhang Chong Zhang Jiaxue Zhang Jiaxue Zhang Yadi Liu Yadi Liu Xiatong Liu Xiatong Liu Xiaorui Guo Xiaorui Guo Hui Li Hui Li Di Liu Di Liu Hai Lu Hai Lu |
| author_sort |
Chong Zhang |
| title |
Integrated Transcriptomic and Proteomic Analysis in the Roadmap of the Xylem Development Stage in Populus tomentosa |
| title_short |
Integrated Transcriptomic and Proteomic Analysis in the Roadmap of the Xylem Development Stage in Populus tomentosa |
| title_full |
Integrated Transcriptomic and Proteomic Analysis in the Roadmap of the Xylem Development Stage in Populus tomentosa |
| title_fullStr |
Integrated Transcriptomic and Proteomic Analysis in the Roadmap of the Xylem Development Stage in Populus tomentosa |
| title_full_unstemmed |
Integrated Transcriptomic and Proteomic Analysis in the Roadmap of the Xylem Development Stage in Populus tomentosa |
| title_sort |
integrated transcriptomic and proteomic analysis in the roadmap of the xylem development stage in populus tomentosa |
| publisher |
Frontiers Media S.A. |
| publishDate |
2021 |
| url |
https://doaj.org/article/cf54e42e14d34b86915d8a946962b069 |
| work_keys_str_mv |
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