Gene expression profile of glioblastoma peritumoral tissue: an ex vivo study.

The gene expression pattern of glioblastoma (GBM) is well documented but the expression profile of brain adjacent to tumor is not yet analysed. This may help to understand the oncogenic pathway of GBM development. We have established the genome-wide expression profiles of samples isolated from GBM t...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Annunziato Mangiola, Nathalie Saulnier, Pasquale De Bonis, Daniela Orteschi, Gigliola Sica, Gina Lama, Benedetta Ludovica Pettorini, Giovanni Sabatino, Marcella Zollino, Libero Lauriola, Anna Colabianchi, Gabriella Proietti, Gyula Kovacs, Giulio Maira, Carmelo Anile
Formato: article
Lenguaje:EN
Publicado: Public Library of Science (PLoS) 2013
Materias:
R
Q
Acceso en línea:https://doaj.org/article/cf557b1b5e8545b1b99f96a7eec52794
Etiquetas: Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
id oai:doaj.org-article:cf557b1b5e8545b1b99f96a7eec52794
record_format dspace
spelling oai:doaj.org-article:cf557b1b5e8545b1b99f96a7eec527942021-11-18T07:54:58ZGene expression profile of glioblastoma peritumoral tissue: an ex vivo study.1932-620310.1371/journal.pone.0057145https://doaj.org/article/cf557b1b5e8545b1b99f96a7eec527942013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23472076/?tool=EBIhttps://doaj.org/toc/1932-6203The gene expression pattern of glioblastoma (GBM) is well documented but the expression profile of brain adjacent to tumor is not yet analysed. This may help to understand the oncogenic pathway of GBM development. We have established the genome-wide expression profiles of samples isolated from GBM tumor mass, white matter adjacent to tumor (apparently free of tumor cells), and white matter controls by using the Affymetrix HG-U133 arrays. Array-CGH (aCGH) was also performed to detect genomic alterations. Among genes dysregulated in peritumoral white matter, 15 were over-expressed, while 42 were down-regulated when compared to white matter controls. A similar expression profile was detected in GBM cells. Growth, proliferation and cell motility/adhesion-associated genes were up-regulated while genes involved in neurogenesis were down-regulated. Furthermore, several tumor suppressor genes along with the KLRC1 (a member of natural killer receptor) were also down-regulated in the peritumoral brain tissue. Several mosaic genomic lesions were detected by aCGH, mostly in tumor samples and several GBM-associated mosaic genomic lesions were also present in the peritumoral brain tissue, with a similar mosaicism pattern. Our data could be explained by a dilution of genes expressed from tumor cells infiltrating the peritumour tissue. Alternatively, these findings could be substained by a relevant amount of "apparently normal" cells presenting a gene profile compatible with a precancerous state or even "quiescent" cancer cells. Otherwise, the recurrent tumor may arise from both infiltrating tumor cells and from an interaction and recruitment of apparently normal cells in the peritumor tissue by infiltrating tumor cells.Annunziato MangiolaNathalie SaulnierPasquale De BonisDaniela OrteschiGigliola SicaGina LamaBenedetta Ludovica PettoriniGiovanni SabatinoMarcella ZollinoLibero LauriolaAnna ColabianchiGabriella ProiettiGyula KovacsGiulio MairaCarmelo AnilePublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 3, p e57145 (2013)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Annunziato Mangiola
Nathalie Saulnier
Pasquale De Bonis
Daniela Orteschi
Gigliola Sica
Gina Lama
Benedetta Ludovica Pettorini
Giovanni Sabatino
Marcella Zollino
Libero Lauriola
Anna Colabianchi
Gabriella Proietti
Gyula Kovacs
Giulio Maira
Carmelo Anile
Gene expression profile of glioblastoma peritumoral tissue: an ex vivo study.
description The gene expression pattern of glioblastoma (GBM) is well documented but the expression profile of brain adjacent to tumor is not yet analysed. This may help to understand the oncogenic pathway of GBM development. We have established the genome-wide expression profiles of samples isolated from GBM tumor mass, white matter adjacent to tumor (apparently free of tumor cells), and white matter controls by using the Affymetrix HG-U133 arrays. Array-CGH (aCGH) was also performed to detect genomic alterations. Among genes dysregulated in peritumoral white matter, 15 were over-expressed, while 42 were down-regulated when compared to white matter controls. A similar expression profile was detected in GBM cells. Growth, proliferation and cell motility/adhesion-associated genes were up-regulated while genes involved in neurogenesis were down-regulated. Furthermore, several tumor suppressor genes along with the KLRC1 (a member of natural killer receptor) were also down-regulated in the peritumoral brain tissue. Several mosaic genomic lesions were detected by aCGH, mostly in tumor samples and several GBM-associated mosaic genomic lesions were also present in the peritumoral brain tissue, with a similar mosaicism pattern. Our data could be explained by a dilution of genes expressed from tumor cells infiltrating the peritumour tissue. Alternatively, these findings could be substained by a relevant amount of "apparently normal" cells presenting a gene profile compatible with a precancerous state or even "quiescent" cancer cells. Otherwise, the recurrent tumor may arise from both infiltrating tumor cells and from an interaction and recruitment of apparently normal cells in the peritumor tissue by infiltrating tumor cells.
format article
author Annunziato Mangiola
Nathalie Saulnier
Pasquale De Bonis
Daniela Orteschi
Gigliola Sica
Gina Lama
Benedetta Ludovica Pettorini
Giovanni Sabatino
Marcella Zollino
Libero Lauriola
Anna Colabianchi
Gabriella Proietti
Gyula Kovacs
Giulio Maira
Carmelo Anile
author_facet Annunziato Mangiola
Nathalie Saulnier
Pasquale De Bonis
Daniela Orteschi
Gigliola Sica
Gina Lama
Benedetta Ludovica Pettorini
Giovanni Sabatino
Marcella Zollino
Libero Lauriola
Anna Colabianchi
Gabriella Proietti
Gyula Kovacs
Giulio Maira
Carmelo Anile
author_sort Annunziato Mangiola
title Gene expression profile of glioblastoma peritumoral tissue: an ex vivo study.
title_short Gene expression profile of glioblastoma peritumoral tissue: an ex vivo study.
title_full Gene expression profile of glioblastoma peritumoral tissue: an ex vivo study.
title_fullStr Gene expression profile of glioblastoma peritumoral tissue: an ex vivo study.
title_full_unstemmed Gene expression profile of glioblastoma peritumoral tissue: an ex vivo study.
title_sort gene expression profile of glioblastoma peritumoral tissue: an ex vivo study.
publisher Public Library of Science (PLoS)
publishDate 2013
url https://doaj.org/article/cf557b1b5e8545b1b99f96a7eec52794
work_keys_str_mv AT annunziatomangiola geneexpressionprofileofglioblastomaperitumoraltissueanexvivostudy
AT nathaliesaulnier geneexpressionprofileofglioblastomaperitumoraltissueanexvivostudy
AT pasqualedebonis geneexpressionprofileofglioblastomaperitumoraltissueanexvivostudy
AT danielaorteschi geneexpressionprofileofglioblastomaperitumoraltissueanexvivostudy
AT gigliolasica geneexpressionprofileofglioblastomaperitumoraltissueanexvivostudy
AT ginalama geneexpressionprofileofglioblastomaperitumoraltissueanexvivostudy
AT benedettaludovicapettorini geneexpressionprofileofglioblastomaperitumoraltissueanexvivostudy
AT giovannisabatino geneexpressionprofileofglioblastomaperitumoraltissueanexvivostudy
AT marcellazollino geneexpressionprofileofglioblastomaperitumoraltissueanexvivostudy
AT liberolauriola geneexpressionprofileofglioblastomaperitumoraltissueanexvivostudy
AT annacolabianchi geneexpressionprofileofglioblastomaperitumoraltissueanexvivostudy
AT gabriellaproietti geneexpressionprofileofglioblastomaperitumoraltissueanexvivostudy
AT gyulakovacs geneexpressionprofileofglioblastomaperitumoraltissueanexvivostudy
AT giuliomaira geneexpressionprofileofglioblastomaperitumoraltissueanexvivostudy
AT carmeloanile geneexpressionprofileofglioblastomaperitumoraltissueanexvivostudy
_version_ 1718422796560760832